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Male factor infertility impacts the rate of mosaic blastocysts in cycles of preimplantation genetic testing for aneuploidy

  • Assisted Reproduction Technologies
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Abstract

Purpose

In this study, we tested the hypothesis that, in PGT-A cycles, decreased semen quality is associated with increased rates of mosaic blastocysts.

Methods

In a retrospective analysis, three hundred and forty PGT-A cycles are divided into study groups according to semen quality. Cycles were initially divided into two groups, discerning couples with absence of male factor of infertility (non-male factor: NMF; N = 146 cycles) from couples with a male factor of infertility (MF; N = 173 cycles). Couples with severe male factor (SMF) infertility (n = 22) were assessed separately. Embryos were cultured to the blastocyst stage and chromosomally assessed by array comparative genomic hybridization (aCGH). The study did not involve specific interventions.

Results

The reproductive outcome of MF and NMF groups did not indicate statistically significant differences. However, while no differences were found between MF and NMF groups in terms of euploid or aneuploid blastocysts rates, a significantly higher rate of mosaic blastocysts was observed in the MF group (3.6% vs. 0.5%, respectively; P = 0.03). A similar pattern of results was observed in the SMF group when compared with those of the other PGT-A cycles taken together (no SMF). In particular, a significantly higher rate of mosaic blastocysts was observed in the SMF group (7.7% and 1.8%, respectively; P = 0.008).

Conclusions

The study outcome strongly suggests that compromised semen quality is associated with increased rates of mosaic blastocysts analysed in PGT-A cycles. Sperm assessment appears therefore as an important factor in the determination of embryo development and for a more precise prognostic assessment of PGT-A cases.

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Authors and Affiliations

  1. 9.Baby - Family and Fertility Center, Via Dante 15, 40125, Bologna, Italy

    Nicoletta Tarozzi, Marco Nadalini, Cristina Lagalla, Giovanni Coticchio, Carlotta Zacà & Andrea Borini

Authors
  1. Nicoletta Tarozzi
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  2. Marco Nadalini
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  3. Cristina Lagalla
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  4. Giovanni Coticchio
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  5. Carlotta Zacà
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  6. Andrea Borini
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Correspondence to Giovanni Coticchio.

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Tarozzi, N., Nadalini, M., Lagalla, C. et al. Male factor infertility impacts the rate of mosaic blastocysts in cycles of preimplantation genetic testing for aneuploidy. J Assist Reprod Genet 36, 2047–2055 (2019). https://doi.org/10.1007/s10815-019-01584-w

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