Abstract
Purpose
The purpose of this study was to identify age-related oocyte or embryo markers suitable for non-invasive analysis, as women over 38 years of age experience diminished pregnancy and ovulation rates.
Methods
We used real-time quantitative PCR to examine the gene expression profiles in cumulus cells acquired from older and younger age groups. We selected 11 genes involved in three functions that directly affect cellular aging: cell cycle control, apoptosis, and metabolism.
Results
CKB and PRDX2 were up-regulated in women older than 38 years, and the expression of these genes in cumulus cells was associated with embryo quality. In good-quality embryos, CKB expression was higher in the cumulus cells acquired from both older and younger age groups than in poor-quality embryos.
Conclusions
These potential relationships among cumulus cell gene expression, oocyte quality, and age may expand our understanding of oogenesis and embryo development. CKB and PRDX2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
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Acknowledgements
We thank Chung-I Chen, Chiu-Ping Chen, Hsiu-Hui Chen, and Ming-Chou Hung for their assistance in laboratory techniques. This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B -039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.
Financial support
This study was supported by a research grant from National Science Council, Taiwan (NSC 93-2314-B-039-017 and NSC 97-2314-B-040-018) to Maw-Sheng Lee.
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Capsule
In cumulus cell gene expression, Creatin kinase B and peroxiredoxin 2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
Where the work was done: Division of Infertility Clinic, Lee Women’s Hospital, Taichung, Taiwan.
Capsule In cumulus cell gene expression, Creatin kinase B and peroxiredoxin 2 may serve as biomarkers or therapeutic targets for the developmental potential of oocytes.
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Lee, MS., Liu, CH., Lee, TH. et al. Association of creatin kinase B and peroxiredoxin 2 expression with age and embryo quality in cumulus cells. J Assist Reprod Genet 27, 629–639 (2010). https://doi.org/10.1007/s10815-010-9459-7
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DOI: https://doi.org/10.1007/s10815-010-9459-7