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Effects of air drying and freezing and long-term storage on phytochemical composition of brown seaweeds

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Abstract

The effect of air drying and freezing at − 25 °C on the total phenolic content, fucoidan, alginate, and amino acids of brown algae Fucus vesiculosus, Fucus distichus, and Ascophyllum nodosum was studied over 365 days of storage every 90 days. The levels of total phenols and polysaccharides in frozen algae samples at 365 days of storage were 75–95% of fresh algae and were higher than in air-dried samples by more than 5–15%. The greatest differences between the fresh and dried samples were in the total phenolic contents. A strong correlation was found between the content of total phenolics and polysaccharides and the water content in frozen samples of F. vesiculosus and A. nodosum. The influence of air drying and freezing processing and storage on the total amino acid content is discussed using F. vesiculosus as an example. The total amino acid content in the air-dried samples increased during storage, but on the 365th day, 99% of the initial content in fresh algae remained. In contrast, the content in the frozen samples had decreased to 82% after the same storage duration. The ratio of the sums of the essential to the total amino acids during storage in the dry and frozen samples increased compared with that in fresh algae from 0.43 to 0.51 after 365 days of storage. Air drying and freezing are suitable procedures in terms of the levels of total phenols, polysaccharides, and amino acids, allowing a shelf life of at least 365 days.

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References

  • Abdollahia M, Axelssona J, Carlssona N-G, Nylundb GM, Albersc E, Undelanda I (2019) Effect of stabilization method and freeze/thaw-aided precipitation on structural and functional properties of proteins recovered from brown seaweed (Saccharina latissima). Food Hydrocoll 96:140–150

    Google Scholar 

  • Aberdein C (1968) The harvest of seaweed. Fish News Int 7:24–28

    Google Scholar 

  • Ar Gall E, Lelchat F, Hupel M, Jégou C, Stiger-Pouvreau V (2015) Extraction and purification of phlorotannins. In: Stengel DB, Connan S (eds) Natural products from marine algae. Springer, New York, pp 131–143

    Google Scholar 

  • Amorim AM, Nardelli AE, Chow F (2020) Effects of drying processes on antioxidant properties and chemical constituents of four tropical macroalgae suitable as functional bioproducts. Journal of Applied Phycology 32(2):1495–1509. https://doi.org/10.1007/s10811-020-02059-7

  • Arslan D, Ozcan MM (2008) Evaluation of drying methods with respect to drying kinetics, mineral content and color characteristics of rosemary leaves. Energy Convers Manag 49:1258–1264

    CAS  Google Scholar 

  • Badmus UO, Taggart MA, Boyd KG (2019) The effect of different drying methods on certain nutritionally important chemical constituents in edible brown seaweeds. J Appl Phycol 31:3883–3897

    CAS  Google Scholar 

  • Bonat Celli G, Ghanem A, Su-Ling BM (2015) Influence of freezing process and frozen storage on the quality of fruits and fruit products. Food Rev Int 32:280–304

    Google Scholar 

  • Buschmann AH, Camus C, Infante J, Neori A, Israel Á, Hernández-González M, Pereda SV, Gomez-Pinchetti JL, Golberg A, Tadmor-Shalev N (2017) Seaweed production: overview of the global state of exploitation, farming and emerging research activity. Eur J Phycol 52:391–406

    Google Scholar 

  • Cardozo KHM, Guaratini T, Barros MP, Falcão VR, Tonon AP, Lopes NP, Campos S, Torres MA, Souza AO, Colepicolo P, Pinto E (2007) Metabolites from algae with economical impact. Comp Biochem Physiol C 146:60–78

    Google Scholar 

  • Carrillo S, Castro MI, Perez-gil F, Rosales E, Manzano RE (1992) The seaweed (Sargassum sinicola Setchel & Gardner) as an alternative for animal feeding. Agric Sci 26:177–181

    Google Scholar 

  • Catarino MD, Silva AMS, Cardoso SM (2018) Phytochemical constituents and biological activities of Fucus spp. Review Mar Drugs 16:249

    Google Scholar 

  • Chang CH, Lin HY, Chang CY, Liu YC (2006) Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. Food Eng 77:478–485

    CAS  Google Scholar 

  • Chaovanalikit A, Wrolstad RE (2004) Anthocyanin and polyphenolic composition of fresh and processed cherries. J Food Sci 69:FCT73–FCT83

    CAS  Google Scholar 

  • Choi J-S, Lee B-B, An S, Sohn JH, Cho KK, ChoiI S (2012) Simple freezing and thawing protocol for long-term storage of harvested fresh Undaria pinnatifida. Fisheries Sci 78:1117–1123

    CAS  Google Scholar 

  • Cornish ML, Garbary DJ (2010) Antioxidants from macroalgae: potential applications in human health and nutrition. Algae 25:155–171

    CAS  Google Scholar 

  • Cox S, Gupta S, Abu-Ghannam N (2012) Effect of different rehydration temperatures on the moisture, content of phenolic compounds, antioxidant capacity and textural properties of edible Irish brown seaweed. LWT-Food Sci Technol 47:300–307

    CAS  Google Scholar 

  • Cronin G, Lindquist N, Hay ME, Fenical W (1995) Effects of storage and extraction procedures on yields of lipophilic metabolites from the brown seaweeds Dictyota ciliolata and D. menstrualis. Mar Ecol Prog Ser 119:265–273

    Google Scholar 

  • Dalmau ME, Bornhorst GM, Eim V, Rossello C, Simal S (2017) Effects of freezing, freeze drying and convective drying on in vitro gastric digestion of apples. Food Chem 215:7–16

    CAS  PubMed  Google Scholar 

  • Dawczynski C, Schubert R, Jahreis G (2007) Amino acids, fatty acids, and dietary fiber in edible seaweed product. Food Chem 103:891–899

    CAS  Google Scholar 

  • De San M (2012) The Farming of Seaweeds. Report SF/2012/28. Indian Ocean Commission/EU SmartFish Programme. Retrieved from http://commissionoceanindien.org/fileadmin/projets/smartfish/Rapport/SF28.pdf 

  • Dermesonlouoglou E, Giannakourou M, Taoukis P (2007) Kinetic modelling of the quality degradation of frozen watermelon tissue: effect of the osmotic dehydration as a pre-treatment. Int J Food Sci Technol 42:790–798

    CAS  Google Scholar 

  • Desrosier NW (2012) Fundamentals of food freezing. Springer, Berlin

    Google Scholar 

  • Duran-Frontera E (2017) Development of a process approach for retaining seaweed sugar kelp (Saccharina latissima) nutrients. Honors Thesis, University of Maine

  • FAO (1976) Production, trade and utilization of seaweeds and seaweed products. FAO Fisheries Technical Paper No. 159; Food and Agriculture Organization of the United Nations: Rome

  • FAO (2018) The state of world fisheries and aquaculture 2018 - meeting the sustainable development goals. Rome

  • Galasso C, Corinaldesi C, Sansone C (2017) Carotenoids from marine organisms: biological functions and industrial applications. Antioxidants 6:33

    Google Scholar 

  • Gao G, Clare AS, Rose C, Caldwell GS (2017) Non-cryogenic preservation of thalli, germlings, and gametes of the green seaweed Ulva rigida. Aquaculture 473:246–250

    Google Scholar 

  • Gupta S, Cox S, Abu-Ghannam N (2011) Effect of different rehydration temperatures on the moisture, content of phenolic compounds, antioxidant capacity and textural properties of edible Irish brown seaweed. LWT-Food Sci Technol 44:1266–1272

    CAS  Google Scholar 

  • Hamdy AEA, Dawes CJ (1988) Proximate constituents and lipid chemistry in two species of Sargassum from the west coast of Florida. Botanica marina, 31:79–82

  • Jeremiah (1996) Freezing effects on food quality. CRC Press, Boca Raton

    Google Scholar 

  • Jordan P, Vilter H (1991) Extraction of proteins from material rich in anionic mucilages: partition and fractionation of vanadate-dependent bromoperoxidases from the brown algae Laminaria digitata and L. saccharina in aqueous polymer two-phase systems. Biochim Biophys Acta 1073:98–106

    CAS  PubMed  Google Scholar 

  • Kadam SU, Álvarez C, Tiwari BK, O’Donnell CP (2015) Chapter 4 - processing of seaweeds. In: Tiwari BK, Troy DJ (eds) seaweed sustainability. Food and non-food applications. Elsevier, London, pp 61–78

    Google Scholar 

  • Kamiloglu S (2020) Industrial freezing effects on the content and bioaccessibility of spinach (Spinacia oleracea L.) polyphenols. J Sci Food Agric 100:4190–4198

    CAS  PubMed  Google Scholar 

  • Kirke DA, Smyth TJ, Rai DK, Kenny O, Stengel DB (2017) The chemical and antioxidant stability of isolated low molecular weight phlorotannins. Food Chem 221:1104–1112

    CAS  PubMed  Google Scholar 

  • Koivikko R, Loponen J, Honkanen T, Jormalainen V (2005) Contents of soluble, cell-wall-bound and exuded phlorotannins in the brown alga Fucus vesiculosus, with implications on their ecological functions. J Chem Ecol 31:195–212

    CAS  PubMed  Google Scholar 

  • Kopjar M, Tiban NN, Pilizota V, Babic J (2009) Stability of anthocyanins, phenols and free radical scavenging activity through sugar addition during frozen storage of blackberries. J Food Process Preserv 33:1–11

    Google Scholar 

  • Kurihara K (2009) Glutamate: from discovery as a food flavor to role as a basic taste (umami). Am J Clin Nutr 90:719–722

    Google Scholar 

  • Lawrenz E, Fedewa EJ, Richardson TI (2011) Extraction protocols for the quantification of phycobilins in aqueous phytoplankton extracts. J Appl Phycol 23:865–871

    Google Scholar 

  • Le Lann K, Jégou C, Stiger-Pouvreau V (2008) Effect of different conditioning treatments on total phenolic content and antioxidant activities in two Sargassacean species: comparison of the frondose Sargassum muticum (Yendo) Fensholt and the cylindrical Bifurcaria bifurcata R. Ross. Phycol Res 56:238–245

    Google Scholar 

  • Lefranc F, Koutsaviti A, Ioannou E, Kornienko A, Roussis V, Kiss R, Newman D (2019) Algae metabolites: from in vitro growth inhibitory effects to promising anticancer activity. Nat Prod Rep 36:810–841

    CAS  PubMed  Google Scholar 

  • Li BB, Smith B, Hossain MM (2006) Extraction of phenolics from citrus peels: I. solvent extraction method. Sep Purif Technol 48:182–188

    CAS  Google Scholar 

  • Ling ALM, Yasir S, Matanjun P, Bakar MFA (2015) Effect of different drying techniques on the phytochemical content and antioxidant activity of Kappaphycus alvarezii. Journal of Applied Phycology 27(4):1717–1723

  • López-Pérez O, del Olmo A, Picon A, Nuñez M (2020) Volatile compounds and odour characteristics during long-term storage of kombu seaweed (Laminaria ochroleuca) preserved by high pressure processing, freezing and salting. LWT 118:108710

    Google Scholar 

  • Mabeau S, Cavaloc E, Fleurence J, Lahaye M (1992) New seaweed based ingredients for the food industry. International Food Ingredients 3:38–45

  • MacArtain P, Gill CIR, Brooks M, Campbell R, Rowland IR (2007) Nutritional value of edible seaweeds. Nutr Rev 65:535–543

    Google Scholar 

  • Milinovic J, Campos B, Mata P (2020) Umami free amino acids in edible green, red, and brown seaweeds from the Portuguese seashore. J Appl Phycol. https://doi.org/10.1007/s10811-020-02169-2

  • Moen E, Larsen B, Ostgaard K, Jensen A (1999) Alginate stability during high salt preservation of Ascophyllum nodosum. J Appl Phycol 11:21–25

    CAS  Google Scholar 

  • Mouritsen OG, Dawczynski C, Duelund L, Jahreis G, Vetter W, Schröder M (2013) On the human consumption of the red seaweed dulse (Palmaria palmata (L.) Weber & Mohr). J Appl Phycol 25:1777–1791

    CAS  Google Scholar 

  • Mouritsen OG, Duelund L, Petersen MA, Hartmann AL, Frøst MB (2019) Umami taste, free amino acid composition, and volatile compounds of brown seaweeds. J Appl Phycol 31:1213–1232

    CAS  Google Scholar 

  • Munda IM (1977) Differences in amino acid composition of estuarine and marine fucoids. Aquat Bot 3:273–280

    CAS  Google Scholar 

  • Ninomiya K (2015) Science of umami taste: adaptation to gastronomic culture. Flavour 4:13

    Google Scholar 

  • Obluchinskaya E (2020) Effect of different post-harvest treatments on free amino acid content in Fucus vesiculosus. KnE Life Sciences 5:386–395

    Google Scholar 

  • Olmo A, Picon A, Nuñez M (2019) High pressure processing for the extension of Laminaria ochroleuca (kombu) shelf-life: a comparative study with seaweed salting and freezing. Innov Food Sci Emerg Technol 52:420–428

    Google Scholar 

  • Pangestuti R, Kim S-K (2011) Biological activities and health benefit effects of natural pigments derived from marine algae. J Funct Foods 3:255–266

    CAS  Google Scholar 

  • Pereira L (2011) A review of the nutrient composition of selected edible seaweeds. In: Pomin VH (ed) Seaweed: ecology, nutrient composition and medicinal uses. Nova Science Publishers, NY, pp 15–50

    Google Scholar 

  • Pereira L (2016) Edible seaweeds of the world. Taylor & Francis, London

    Google Scholar 

  • Perry JJ, Brodt A, Skonberg DI (2019) Influence of dry salting on quality attributes of farmed kelp (Alaria esculenta) during long term refrigerated storage. LWT Food Sci Technol 114:108362

    CAS  Google Scholar 

  • Pomin VH (2012) Seaweed. Ecology, nutrient composition and medicinal uses. Nova science, NY

    Google Scholar 

  • Poojary MM, Orlien V, Olsen K (2019) Conventional and enzyme-assisted green extraction of umami free amino acids from Nordic seaweeds. J Appl Phycol 31:3925–3939

    Google Scholar 

  • Rickman JC, Barrett DM, Bruhn CM (2007) Nutritional comparison of fresh, frozen and canned fruits and vegetables. Part 1. Vitamins C and B and phenolic compounds. J Sci Food Agric 87:930–944

    CAS  Google Scholar 

  • Rodrigues MS (2015) Caracterização da Composição nutricional da macroalga Fucus vesiculosus e aalteração dos compostos bioativosnos diferentes métodos de secagem. Masters Thesis. Instituto Politecnico Bragança

  • Rodríguez-Jasso RM, Mussatto SI, Pastrana L, Aguilar CN, Teixeira JA (2013) Extraction of sulfated polysaccharides by autohydrolysis of brown seaweed Fucus vesiculosus. J Appl Phycol 25:31–39

    Google Scholar 

  • Romero JB, Villanueva RD, Nemesio M, Montaño E (2008) Stability of agar in the seaweed Gracilaria eucheumatoides (Gracilariales, Rhodophyta) during postharvest storage. Bioresour Technol 99:8151–8155

    CAS  PubMed  Google Scholar 

  • Sánchez-Machado DI, López-Cervantes J, López-Hernández J, Paseiro-Losada P (2004) Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chem 85:439–444

    Google Scholar 

  • Stévant P, Rebours C, Chapman A (2017) Seaweed aquaculture in Norway: recent industrial developments and future perspectives. Aquacult Int 25:1373–1390

    Google Scholar 

  • Stévant P, Indergard E, Olafsclottir A, Marfaing H, Larssen WE, Fleurence J, Roleda MY, Rustad T, Slizyte R, Nordtvedt TS (2018) Effects of drying on the nutrient content and physico-chemical and sensory characteristics of the edible kelp Saccharina latissima. J Appl Phycol 30:2587–2599

    Google Scholar 

  • Suzuki T, Ohsugi Y, Yoshie Y, Shirai T, Hirano T (1996) Dietary fiber content, water holding capacity and binding capacity of seaweeds. Fish Sci 62:454–461

    CAS  Google Scholar 

  • Tabarsa M, Rezaei M, Ramezanpour Z, Waaland JR, RezaRabiei R (2012) Fatty acids, amino acids, mineral contents, and proximate composition of some brown seaweeds. J Phycol 48:285–292

    CAS  PubMed  Google Scholar 

  • Taylor R, Fletcher RL (1998) Cryopreservation of eukaryotic algae – a review of methodologies. J Appl Phycol 10:481–501

    Google Scholar 

  • Usov AI, Smirnova GP, Klochkova NG (2001) Polysaccharides of algae, 55.1. Polysaccharide composition of several brown algae from Kamchatka. Russ J Bioorg Chem 27:395–399

    CAS  Google Scholar 

  • Visch W, Rad-Menendez C, Nylund GM, Pavia H, Ryan MJ, Day J (2019) Underpinning the development of seaweed biotechnology: cryopreservation of brown algae (Saccharina latissima) gametophytes. Biopreserv Biobank 17:1–9

    Google Scholar 

  • Wells ML, Potin P, Craigie JS (2017) Algae as nutritional and functional food sources: revisiting our understanding. J Appl Phycol 29:949–982

    CAS  PubMed  Google Scholar 

  • Wong K, Cheung PC (2001) Influence of drying treatment on three Sargassum species. J Appl Phycol 13:43–50

    CAS  Google Scholar 

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Acknowledgments

This study was conducted within the framework of the Government Assignment, project no. 0228-2018-0005, and State Registry no. 01201453843 to the Murmansk Marine Biological Institute Russian Academy of Sciences, for the topic Scientific Basics of Innovative Technologies of BAS from Algae of the Barents Sea (basic funding from the Federal Agency for Scientific Organizations).

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  1. Department of Zoobentos, Research Group of Biochemistry and Technology of Seaweeds, Murmansk Marine Biological Institute Russian Academy of Sciences, Vladimirskaya St. 17, Murmansk, 183010, Russia

    Ekaterina Obluchinskaya & Anna Daurtseva

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  1. Ekaterina Obluchinskaya
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Correspondence to Ekaterina Obluchinskaya.

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Obluchinskaya, E., Daurtseva, A. Effects of air drying and freezing and long-term storage on phytochemical composition of brown seaweeds. J Appl Phycol 32, 4235–4249 (2020). https://doi.org/10.1007/s10811-020-02225-x

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