Skip to main content

Advertisement

SpringerLink
Log in

Thiol/disulfide homeostasis in patients with ocular-active and ocular-inactive Behçet disease

  • Original Paper
  • Published:
International Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To evaluate thiol/disulfide homeostasis in ocular-active (OA) and ocular-inactive (OI) Behçet disease (BD) patients and compare the data with healthy subjects.

Methods

Twenty OABD patients, 20 OIBD patients and 20 healthy control subjects were included into the study. The BD ocular attack score 24 (BOS24) scoring system was used to assess the activity of disease in ocular BD patients. Systemic activity was also evaluated using BD current activity form (BDCAF). The native thiol (NT), total thiol (TT) and disulfide levels and NT/TT, disulfide/NT and disulfide/TT ratios were measured via using an innovative and automated method.

Results

BOS24 and BDCAF scores were 13.25 ± 2.32 and 4.18 ± 2.06 in OABD patients and 0.31 ± 0.47 and 2.14 ± 1.98 in OIBD patients, respectively. The NT, TT levels and NT/TT ratio were significantly reduced; in contrast, the disulfide levels, disulfide/NT and disulfide/TT ratios were significantly increased in OABD and OIBD patients compared to the healthy control subjects (p < 0.05). Moreover, while the levels of NT and TT were significantly reduced, the disulfide levels as well as disulfide/NT and disulfide/TT ratios were significantly elevated between OABD and OIBD patients (p < 0.05). However, the ratio of NT/TT did not significantly differ between OABD and OIBD patients (p = 0.449). The multiple regression model including BOS24 and BDCAF score statistically significantly predicted NT level, TT level and disulfide level (p < 0.001 for all).

Conclusion

Thiol oxidation in BD patients resulted in a change of the thiol/disulfide balance. Therefore, thiol/disulfide homeostasis in BD patients can be used an innovative oxidative stress marker.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. Mendoza-Pinto C, Garcia-Carrasco M, Jimenez-Hernandez M et al (2010) Etiopathogenesis of Behçet’s disease. Autoimmun Rev 9:241–245. https://doi.org/10.1016/j.autrev.2009.10.005

    Article  CAS  PubMed  Google Scholar 

  2. Hirohata S, Kikuchi H (2003) Behçet’s disease. Arthritis Res Ther 5:139–146. https://doi.org/10.1186/ar757

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Sepici Dinçel A, Özkan Y, Yardım-Akaydın S et al (2006) The association between total antioxidant status and oxidative stress in Behçet’s disease. Rheumatol Int 26:1005–1009. https://doi.org/10.1007/s00296-006-0126-3

    Article  CAS  PubMed  Google Scholar 

  4. Harzallah O, Kerkeni A, Baati T et al (2008) Oxidative stress: correlation with Behçet’s disease duration, activity and severity. Eur J Int Med 19:541–547. https://doi.org/10.1016/j.ejim.2008.08.001

    Article  CAS  Google Scholar 

  5. Onur E, Kabaroglu C, Inanir I et al (2011) Oxidative stress impairs endothelial nitric oxide levels in Behçet’s disease. Cutan Ocul Toxicol 30:217–220. https://doi.org/10.3109/15569527.2011.554480

    Article  CAS  PubMed  Google Scholar 

  6. Cejkova J, Stipek S, Crkovska J et al (2004) UV rays, the pro-oxidant/anti-oxidant imbalance in the cornea and oxidative eye damage. Physiol Res 53:1–10

    CAS  PubMed  Google Scholar 

  7. Biswas S, Chida AS, Rahman I (2006) Redox modifications of protein-thiols: emerging roles in cell signaling. Biochem Pharmacol 71:551–564. https://doi.org/10.1016/j.bcp.10.044

    Article  CAS  PubMed  Google Scholar 

  8. Turell L, Radi R, Alvarez B (2013) The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med 65:244–253. https://doi.org/10.1016/j.freeradbiomed.2013.05.050

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Gulpamuk B, Tekin K, Sonmez K et al (2017) The significance of thiol/disulfide homeostasis and ischemia-modified albumin levels to assess the oxidative stress in patients with different stages of diabetes mellitus. Scand J Clin Lab Invest 78:136–142. https://doi.org/10.1080/00365513.2017.1422540

    Article  CAS  Google Scholar 

  10. Go YM, Jones DP (2011) Cysteine/cystine redox signaling in cardiovascular disease. Free Radic Biol Med 50:495–509. https://doi.org/10.1016/j.freeradbiomed.2010.11.029

    Article  CAS  PubMed  Google Scholar 

  11. Tetik S, Ahmad S, Alturfan AA et al (2010) Determination of oxidant stress in plasma of rheumatoid arthritis and primary osteoarthritis patients. Indian J Biochem Biophys 47:353–358

    CAS  PubMed  Google Scholar 

  12. Altinkaynak H, Kurkcuoglu PZ, Caglayan M et al (2018) A novel marker in acute central serous chorioretinopathy: thiol/disulfide homeostasis. Int Ophthalmol 38:175–181. https://doi.org/10.1007/s10792-017-0444-3

    Article  PubMed  Google Scholar 

  13. Arıkan Yorgun M, Toklu Y, Altınkaynak H et al (2016) A novel tool for the assessment oxidative stress in age-related macular degeneration: thiol/disulfide homeostasis revisited. Cur Eye Res 41:1584–1589. https://doi.org/10.3109/02713683.2016.1141965

    Article  CAS  Google Scholar 

  14. Tanaka R, Murata H, Takamoto M et al (2016) Behçet’s disease ocular attack score 24 and visual outcome in patients with Behçet’s disease. Br J Ophthalmol 100:990–994. https://doi.org/10.1136/bjophthalmol-2015-307362

    Article  PubMed  Google Scholar 

  15. International Team for the revision of the International criteria for Behçet’s disease (ITR-ICBD) (2014) The International Criteria for Behçet’s disease (ICBD): a collaborative study of 27 countries on sensitivity and specificity of the new criteria. J Eur Acad Dermatol Venereol 28:338–347. https://doi.org/10.1111/jdv.12107

    Article  Google Scholar 

  16. Lawton G, Bhakta BB, Chamberlain MA et al (2004) The Behçet’s disease activity index. Rheumatology (Oxford) 43:73–78. https://doi.org/10.1093/rheumatology/keg453

    Article  CAS  Google Scholar 

  17. Erel O, Neselioglu S (2014) A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 47:326–332. https://doi.org/10.1016/j.clinbiochem.2014.09.026

    Article  CAS  PubMed  Google Scholar 

  18. Dotan Y, Lichtenberg D, Pinchuk I (2004) Lipid peroxidation cannot be used as a universal criterion of oxidative stress. Prog Lipid Res 43:200–227. https://doi.org/10.1016/j.plipres.2003.10.001

    Article  CAS  PubMed  Google Scholar 

  19. Therond P, Bonnefont-Rousselot D, Davit-Spraul A et al (2000) Biomarkers of oxidative stress: an analytical approach. Curr Opin Clin Nutr Metab Care 3:373–384. https://doi.org/10.1097/00075197-200009000-00009

    Article  CAS  PubMed  Google Scholar 

  20. Kokcam I, Nazıroglu M (2002) Effects of vitamin E supplementation on blood antioxidants levels in patients with Behcet’s disease. Clin Biochem 35:633–639. https://doi.org/10.1016/s0009-9120(02)00400-9

    Article  CAS  PubMed  Google Scholar 

  21. Yazici C, Kose K, Calis M et al (2004) Increased advanced oxidation protein products in Behcet’s disease: a new activity marker. Br J Dermatol 151(July):105. https://doi.org/10.1011/j.1365-2133-2004.06003.x

    Article  CAS  PubMed  Google Scholar 

  22. Bekpinar S, Kilic N, Unlucerci Y et al (2005) Evaluation of nitrosative and oxidative stress in Behcet disease. J Eur Acad Dermatol Venereol 19:167–171. https://doi.org/10.1111/j.1468-3083.2005.01075.x

    Article  CAS  PubMed  Google Scholar 

  23. Atmaca G (2004) Antioxidant effects of sulfur-containing amino acids. Yonsei Med J 45:776–788. https://doi.org/10.3349/ymj.2004.45.5.776

    Article  CAS  PubMed  Google Scholar 

  24. Halliwell B, Whiteman M (2004) Measuring RS and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 142:231–255. https://doi.org/10.1038/sj.bjp.070.5776

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Emmi G, Silvestri E, Squatrito D et al (2014) Behçet’s syndrome pathophysiology and potential therapeutic targets. Intern Emerg Med 9:257–265. https://doi.org/10.1007/s11739-013-1036-5

    Article  PubMed  Google Scholar 

  26. Niwa Y, Miyake S, Sakane T (1982) Autooxidative damage in Behçet’s disease- endothelial cell damage following the elevated oxygen radicals generated by stimulated neutrophils. Clin Exp Immunol 49:247–255

    CAS  PubMed  PubMed Central  Google Scholar 

  27. Chambers JC, Haskard DO, Kooner JS (2001) Vascular endothelial function and oxidative stress mechanisms in patients with Behçet’s syndrome. Am J Coll Cardiol 37:417–420. https://doi.org/10.1016/s735-1097(00)01137-2

    Article  Google Scholar 

Download references

Funding

No financial support was received for this study.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Faculty of Medicine, Fırat University, Elazig, Turkey

    Mehmet Balbaba, Hakan Yıldırım & Ali Dal

  2. Department of Ophthalmology, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey

    Fatih Ulaş & Adem Soydan

  3. Department of Biochemistry, Faculty of Medicine, Fırat University, Elazig, Turkey

    Süleyman Aydın

Authors
  1. Mehmet Balbaba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fatih Ulaş
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hakan Yıldırım
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Adem Soydan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ali Dal
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Süleyman Aydın
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MB contributed to concept, design, intellectual content and drafted the article. FU contributed to design, concept and statistical analysis. HY acquired data and interpreted data. AS contributed to data analysis and intellectual content. AD acquired data, drafted the article and interpreted data. SA contributed to data analysis.

Corresponding author

Correspondence to Mehmet Balbaba.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of Fırat University Faculty of Medicine ethics committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Balbaba, M., Ulaş, F., Yıldırım, H. et al. Thiol/disulfide homeostasis in patients with ocular-active and ocular-inactive Behçet disease. Int Ophthalmol 40, 2643–2650 (2020). https://doi.org/10.1007/s10792-020-01445-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10792-020-01445-x

Keywords

Search

Navigation