Abstract
Ischaemia and reperfusion (I/R)-induced gastrointestinal disorders are caused by free radicals, resulting in organ damage and functional disarrangement. This study aimed to investigate the healing effects of hydroalcoholic extracts from the leaves of Eugenia punicifolia (Kunth) DC. (HEEP) in male and female Wistar rats with I/R-induced peptic injuries, and the role of antioxidants in improving this response. After I/R-induced gastric and duodenal injuries, male and female [intact (INT) and ovariectomized (OVZ)] rats were orally treated with HEEP for 6 days. Biochemical analysis was used to determine the catalase (CAT), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities, as well as malondialdehyde and reduced glutathione levels, to measure the gastric and duodenal healing process. Six days of HEEP treatment significantly decreased the I/R-induced gastric [male (73.68%), INT (52.83%), and OVZ (43.13%)] and duodenal damage [male (57.03%), INT (56.04%), and OVZ (54.83%)] in all groups. In OVZ rats, the healing effect of HEEP occurred because of the increased activity of SOD (2x) and CAT (1.16x) in the gastric mucosa. In the duodenal mucosa of INT rats, the extract reduced MPO (20.83%) activity. The 6-day HEEP treatment improved the healing of I/R-induced peptic ulcer injury, with the system acting differently in males and females. The antioxidant system is an important component of the HEEP activity during post-I/R mucosal recovery. This result revealed the importance of antioxidant compounds in minimizing the severity of I/R-related events.
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Funding
This study was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (grants number: 2009/52237-90; 2015/14979-3 and 2017/15610-0), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) [grant numbers 305570/2012-9 to Vilegas, W and 306209/2016-0 to Hiruma-Lima, CA], and the Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES, Brazil) [grant number: 88882.183585/2018-01 to Rodrigues]. The authors thank FAPESP, CNPq, and CAPES for the funding of this study.
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Périco, LL, Santos, RC and Rodrigues VP performed the experiments. Nunes, VVA, and dos Santos C prepared Eugenia punicifolia extract. Périco, LL, Santos, RC, da Rocha, LR, Vilegas, W, and Hiruma-Lima, CA, contributed to the study's conception, design, and coordination. Périco LL, Rodrigues VP, Santos RC, da Rocha LR, and Hiruma-Lima CA participated in writing the manuscript. All authors have approved the final version of the manuscript for publication.
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Lucena Périco, L., de Cássia dos Santos, R., Peixoto Rodrigues, V. et al. Role of the antioxidant pathway in the healing of peptic ulcers induced by ischemia–reperfusion in male and female rats treated with Eugenia punicifolia. Inflammopharmacol 30, 1383–1394 (2022). https://doi.org/10.1007/s10787-022-00946-8
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DOI: https://doi.org/10.1007/s10787-022-00946-8