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Anti-inflammatory and immunomodulatory effects of RDV-8 [C18H22N2O2S (ethyl 1-butyl-6-methyl-2-phenyl-4-thioxo-1,4-dihydropyrimidine-5-carboxylate)] in a rat model of induced pleurisy and in vitro lymphoproliferation

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Abstract

RDV-8 [C18H22N2O2S (ethyl 1-butyl-6-methyl-2-phenyl-4-thioxo-1,4-dihydropyrimidine-5-carboxylate)] is derived from the 4-thioxopyrimidine, and presents important clinical effects. The present study explored the RDV-8 effects in the proliferation of human peripheral blood mononuclear cells (PBMCs), as well as in a pleurisy-induced rat model. PBMCs were directly plated in four different RDV-8 concentrations (0.0125, 0.025, 0.05 and 0.1 mg/mL). RDV-8 decreased cell proliferation and monocyte chemotactic protein 1 synthesis. The interleukin 1 levels and the cytotoxic effect were not significantly affected by RDV-8 treatment. In the carrageenan-induced pleurisy model, the RDV-8 (3 mg/kg) treatment induced a significant reduction in the exudate volume, in the polymorphonuclear leukocyte migration and in the pleural exudate NO levels. The results indicate that RDV-8 may have an immunomodulatory effect, as well as anti-inflammatory actions suggesting that it could represent a new strategy in the inflammatory response modulation.

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Authors and Affiliations

  1. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, Prédio 12, Bloco C, Sala 263, CEP 90.619–900, Porto Alegre, Rio Grande do Sul, Brazil

    Marcos Schuch Azambuja, Adroaldo Lunardelli, Robson Henrich Amaral, Fernanda Bordignon Nunes, Eduardo Caberlon, Vinicius Lorini da Costa, Márcio Vinícius Fagundes Donadio, Denizar Alberto da Silva Melo & Jarbas Rodrigues de Oliveira

  2. Faculdade de Enfermagem Nutrição e Fisioterapia, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Rio Grande do Sul, Brazil

    Márcio Vinícius Fagundes Donadio & Denizar Alberto da Silva Melo

  3. Instituto de Química, Universidade Federal da Bahia (UFBA), Salvador, Bahia, Brazil

    Daniela Nunes Vitor & Silvio Cunha

Authors
  1. Marcos Schuch Azambuja
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  2. Adroaldo Lunardelli
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  3. Robson Henrich Amaral
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  4. Fernanda Bordignon Nunes
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  5. Eduardo Caberlon
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  6. Vinicius Lorini da Costa
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  7. Márcio Vinícius Fagundes Donadio
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  8. Daniela Nunes Vitor
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  9. Denizar Alberto da Silva Melo
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  10. Silvio Cunha
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  11. Jarbas Rodrigues de Oliveira
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Corresponding author

Correspondence to Jarbas Rodrigues de Oliveira.

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Azambuja, M.S., Lunardelli, A., Amaral, R.H. et al. Anti-inflammatory and immunomodulatory effects of RDV-8 [C18H22N2O2S (ethyl 1-butyl-6-methyl-2-phenyl-4-thioxo-1,4-dihydropyrimidine-5-carboxylate)] in a rat model of induced pleurisy and in vitro lymphoproliferation. Inflammopharmacol 19, 145–153 (2011). https://doi.org/10.1007/s10787-010-0063-3

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  • DOI: https://doi.org/10.1007/s10787-010-0063-3

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