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Combination of Enteral Nutrition and Probiotics Promote Recovery Following Ileal Pouch–Anal Anastomosis in Rats

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Abstract

Many patients with ulcerative colitis suffer from malnutrition and intestinal flora disorders, which affect the postoperative intestinal barrier function of the ileal pouch. This study aimed to investigate the effects of enteral nutrition combined with probiotics after ileal pouch–anal anastomosis in rats. Male Sprague–Dawley rats underent ileal pouch–anal anastomosis and were randomly assigned to a control group (standard rat chow), enteral nutrition group (short-peptide enteral nutrition), or probiotic nutrition group (short-peptide enteral nutrition and Lactobacillus acidophilus). The primary outcomes were a histological score and occludin levels in the ileal pouch. The secondary outcomes were nutritional status and fecal flora distribution. The histological scores in the control group were significantly higher than in the enteral nutrition and probiotic nutrition groups (P < 0.05), while occludin levels were significantly lower in the controls compared with the other two groups (P < 0.05). Serum total protein, albumin, transthyretin, and transferrin levels were significantly higher in the probiotic nutrition group, followed by the enteral nutrition and control groups (all P < 0.05). Total fecal flora, and Gram-positive and Gram-negative rods differed significantly among the groups (all P < 0.05), but there were no significant differences in Gram-positive or Gram-negative cocci (all P > 0.05). Enteral nutrition combined with probiotics can effectively protect the intestinal barrier function of the ileal pouch in rats, possibly via the stable distribution of the intestinal flora and good nutritional status.

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Data Availability

Availability of data and material is not applicable for this study.

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Acknowledgments

We thank Susan Furness, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript.

Code availability

Code availability is not applicable for this study.

Funding

This work was supported by a grant from the Li Jie-shou Gut Barrier Foundation (Grant No. LJS_201008).

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Authors and Affiliations

  1. Department of General Surgery, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin, 300052, China

    Ting Zheng, Zongran Chen, Xin Wang, Jian Liu & Gang Liu

  2. Department of Intensive Care Unit, Tianjin Medical University General Hospital, Tianjin, 300052, China

    Ying Gao

  3. Department of General Surgery, The Second Hospital of Anhui Medical University, Hefei, 230601, Anhui, China

    Yanyan Xu

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  1. Ting Zheng
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  7. Gang Liu
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Yanyan Xu, Zongran Chen, and Xin Wang. The first draft of the manuscript was written by Ting Zheng and Ying Gao. Jian Liu and Gang Liu revised it critically for important intellectual content. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Gang Liu.

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The experiments were reviewed and approved by the Animal Ethics and Welfare Committee (approval number: TMUaMEC2017001).

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The authors declare that they have no conflict of interest.

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Ting Zheng and Ying Gao contributed to the work equally and should be regarded as co-first authors.

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Zheng, T., Gao, Y., Xu, Y. et al. Combination of Enteral Nutrition and Probiotics Promote Recovery Following Ileal Pouch–Anal Anastomosis in Rats. Inflammation 44, 725–736 (2021). https://doi.org/10.1007/s10753-020-01372-0

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  • DOI: https://doi.org/10.1007/s10753-020-01372-0

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