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Co-Nanoencapsulation of Vitamin D3 and Curcumin Regulates Inflammation and Purine Metabolism in a Model of Arthritis

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Abstract

We analyzed the effects of a nanoencapsulated association of curcumin and vitamin D3 on purine metabolism enzymes in neutrophils, lymphocytes, and platelets in a model of adjuvant-induced arthritis (AIA) in rats. Following AIA induction, the animals were treated for 15 days with free and nanoencapsulated curcumin (4 mg/kg), nanocapsules of vitamin D3 (VD3) (15.84 IU/day), a nanoencapsulated combination of curcumin and VD3, vehicle, or blank nanocapsules. The animals were euthanized, and blood was collected to evaluate the activities of E-NTPDase, adenosine deaminase (ADA), and myeloperoxidase (MPO), as well as reactive oxygen species (ROS) levels and biochemical parameters. Also, the liver and kidney were collected for histological analysis. The changes in the activities of purinergic enzymes indicated that inflammation was significantly reverted by vitamin D3 and curcumin co-nanoencapsulation treatments in the arthritic rats. The reduction of inflammation was confirmed by the reduction in the signs and symptoms of AIA, as well as in MPO activity by all formulations. The treatments with nanocapsules reverted histological alterations in the kidney. Serum parameters were not altered by the induction or treatments. Our results suggest that co-nanoencapsulation of vitamin D3 and curcumin is an efficient alternative adjuvant treatment for rheumatoid arthritis as it reverts the changes in the purine metabolism and reduces arthritis-associated inflammation.

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Acknowledgments

The authors would like to thank Professor Volnei Teixeira at the Unijuí for his support in the interpretation of histology.

Funding

This study was partly financed by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)—Financial Code 001 (PROEX number: 88882.182182/2018-01).

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  1. Laboratório de Imunobiologia Experimental e Aplicada (LABIBIO), Departamento de Microbiologia e Parasitologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, Av. Roraima, 1000, prédio 20, Santa Maria, RS, 97105-900, Brazil

    Jean Lucas G. da Silva, Daniela F. Passos, Viviane M. Bernardes, Fernanda L. Cabral, Paulo G. Schimites, Alessandra G. Manzoni, Matheus H. Jantsch & Daniela B. R. Leal

  2. Programa de Pós-Graduação em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Jean Lucas G. da Silva, Daniela F. Passos, Viviane M. Bernardes, Alessandra G. Manzoni & Daniela B. R. Leal

  3. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Fernanda L. Cabral, Paulo G. Schimites & Cristiane de Bona da Silva

  4. Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

    Edilene Gadelha de Oliveira & Ruy Carlos Ruver Beck

  5. Departamento de Patologia, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil

    Roberto M. Maciel

Authors
  1. Jean Lucas G. da Silva
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  2. Daniela F. Passos
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  3. Viviane M. Bernardes
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  4. Fernanda L. Cabral
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  5. Paulo G. Schimites
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  6. Alessandra G. Manzoni
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  7. Edilene Gadelha de Oliveira
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  8. Cristiane de Bona da Silva
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  9. Ruy Carlos Ruver Beck
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  10. Matheus H. Jantsch
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  11. Roberto M. Maciel
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  12. Daniela B. R. Leal
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Corresponding author

Correspondence to Daniela B. R. Leal.

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The experimental protocol was approved by the Ethics Committee on the Use of Animal at the Federal University of Santa Maria (8937281117).

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da Silva, J.L.G., Passos, D.F., Bernardes, V.M. et al. Co-Nanoencapsulation of Vitamin D3 and Curcumin Regulates Inflammation and Purine Metabolism in a Model of Arthritis. Inflammation 42, 1595–1610 (2019). https://doi.org/10.1007/s10753-019-01021-1

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