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Fenton Reaction-Generated Advanced Oxidation Protein Products Induces Inflammation in Human Embryonic Kidney Cells

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Abstract

Fenton reaction is a new mechanism able to generate advanced oxidation protein products (AOPPs) by exposing the human serum albumin to the Fenton system. Here, we characterized the effects of Fenton reaction-generated advanced oxidation protein products (AOPP-FR) on the gene transcription of the nuclear factor-κB (NF-κB), cyclooxygenase-2 (COX-2), and interleukin-6 (IL-6) in human embryonic kidney cells (HEK 293). To investigate the effects of AOPP-FR and AOPP-HOCl on transcription of inflammatory genes, the NF-κB, COX-2, and IL-6 luciferase promoter activities were analyzed. AOPP-FR and AOPP-HOCl were able to induce the activation of the gene transcription of NF-κB, COX-2, and IL-6 in HEK 293 cells. However, the effects of AOPP-FR were significantly higher than the effects of AOPP-HOCl in relation to COX-2 and IL-6. AOPP-FR induces the activation of the gene transcription of NF-κB, COX-2, and IL-6 and may represent a novel pathogenic mediator of inflammation in kidney.

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Abbreviations

AOPPs:

Advanced oxidation protein products

AOPP-FR:

Fenton reaction-generated advanced oxidation protein products

AOPP-HOCl:

Hypochlorous acid-generated advanced oxidation protein products

COX-2:

Cyclooxygenase-2

HEK 293:

Human embryonic kidney cells

HO• :

Hydroxyl radical

HOC:

Hypochlorous acid

HSA:

Human serum albumin

IL-6:

Interleukin-6

MPO:

Myeloperoxidase

NADPH:

Nicotinamide adenine dinucleotide phosphate

NF-κB:

Nuclear factor-κB

ROS:

Reactive oxygen species

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Acknowledgments

This study was supported by grants from the CAPES/DGU (BEX 4422/09-0 and 3449/11-4), Brazil, IDIBELL, and the Secretaría de Estado de Universidades, Ministerio de Ciencia e Innovación (PHB2008-0080-PC), Spain. The authors thank CNPq/Brazil and CAPES/Brazil for providing scholarships.

Author information

Authors and Affiliations

  1. Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, Brazil

    Guilherme Vargas Bochi, Vanessa Dorneles Torbitz & Rafael Noal Moresco

  2. Pharmacology Postgraduate Program, Health Sciences Center, Federal University of Santa Maria, Santa Maria, Brazil

    Guilherme Vargas Bochi & Rafael Noal Moresco

  3. Centro de Ciências da Saúde, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria, Avenida Roraima 1000, Prédio 26, Sala 1401, Camobi, 97105-900, Santa Maria, RS, Brasil

    Guilherme Vargas Bochi

  4. Research Laboratory of Clinical Microbiology, Franciscan University Center, Santa Maria, Brazil

    Roberto Christ Vianna Santos

  5. Unitat Bioquímica i Biologia Molecular, Departament de Ciències Fisiològiques II, Institut d’Investigació Biomèdica de Bellvitge (IDIBELL), Campus de Bellvitge, Universitat de Barcelona, Barcelona, Spain

    Monica Cubillos-Rojas & José Luis Rosa López

  6. Laboratório de Genética e Ecotoxicologia Molecular, Programa de Pós-Graduação em CiênciasAmbientais, Universidade Comunitária da Região de Chapecó, Chapecó, Brazil

    Anna Maria Siebel

  7. Nanosciences Postgraduate Program, Franciscan University Center, Santa Maria, Brazil

    Patrícia Gomes

  8. Research Laboratory of Cellular Biophysics and Inflammation, The Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, Brazil

    Jarbas Rodrigues de Oliveira

Authors
  1. Guilherme Vargas Bochi
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  2. Vanessa Dorneles Torbitz
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Corresponding author

Correspondence to Guilherme Vargas Bochi.

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Bochi, G.V., Torbitz, V.D., Santos, R.C.V. et al. Fenton Reaction-Generated Advanced Oxidation Protein Products Induces Inflammation in Human Embryonic Kidney Cells. Inflammation 39, 1285–1290 (2016). https://doi.org/10.1007/s10753-016-0360-0

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