Abstract
As a result of increased anthropogenic nitrogen (N) loading in surface waters of agricultural watersheds, there is enhanced interest to understand and quantify N removal mechanisms. Denitrification, an important N removal mechanism in aquatic systems, may contribute to reducing N pollution in agricultural headwater streams. However, the key factors controlling this process in lotic systems remain unclear. The objective of our study was to examine the factors regulating rates of denitrification in the sediments of agricultural headwater streams in the mid-western USA. Denitrification rates were variable among streams and treatments (<0.1–28.0 μg N g AFDM−1 h−1) and on average, were higher than those reported for similar headwater streams. Carbon quantity and quality, and pH had no effect on denitrification, while temperature and nitrate ( \( {\text{NO}}^{ - }_{{\text{3}}} \)) concentrations had a positive effect on rates of denitrification. Specifically, \( {\text{NO}}^{ - }_{{\text{3}}} \) controlled denitrification following Michaelis-Menten kinetics. We calculated a value of km (1.0 mg \( {\text{NO}}^{ - }_{{\text{3}}} \)-N L-1) that was comparable to other studies in aquatic sediments but was well below the median in-stream \( {\text{NO}}^{ - }_{{\text{3}}} \) concentrations (5.2–17.4 mg \( {\text{NO}}^{ - }_{{\text{3}}} \)-N L−1) observed at the study sites. Despite high rates of denitrification, this removal mechanism is most likely \( {\text{NO}}^{ - }_{{\text{3}}} \) saturated in the agricultural headwater streams we examined, suggesting that these systems are not effective at removing in-stream N.
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Acknowledgments
We thank two anonymous reviewers and the Associate Editor for their valuable comments on the manuscript. Special thanks to Lance Williams, Anne Carey, and Tom Koontz whose suggestions greatly improved the manuscript. We would also like to thank Gwen Dubelko, Becky Fauver, and Nickla Louisy for assistance in the laboratory and the field, and Scott Long for obtaining site permissions. This research was supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (award # 2005-35102-16325) and the Water Resources Research Institute of the USGS.
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Herrman, K.S., Bouchard, V. & Moore, R.H. Factors affecting denitrification in agricultural headwater streams in Northeast Ohio, USA. Hydrobiologia 598, 305–314 (2008). https://doi.org/10.1007/s10750-007-9164-4
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DOI: https://doi.org/10.1007/s10750-007-9164-4