Abstract
Secretory coils and ducts are two components of eccrine sweat glands with different structures and functions. In our previous study, we combined keratins and α-SMA to distinguish between secretory coils and ducts. However, the key deficiency of the method was that none of the antibodies used was specific for ducts. In this study, we first examined the co-localization of K5/K7, α-SMA/K14, K7/S100P and α-SMA/S100A2 by double-immunofluorescence staining to confirm the localization of S100P and S100A2 in native human eccrine sweat glands, and second we identified secretory coil-like and duct-like structures in the 3D reconstituted eccrine sweat gland spheroids by double-immunofluorescence staining for K7/S100P and α-SMA/S100A2. In native human eccrine sweat glands, S100A2 immunoreactivity was confined to the outer layer and S100P to the inner layer of the duct. In 12-week Matrigel plugs containing eccrine sweat gland cells, double-immunofluorescence staining for K7/S100P and α-SMA/S100A2 could easily distinguish duct-like structures from secretory coil-like structures. We conclude that S100A2 and S100P can be used as specific duct markers in eccrine sweat glands, and combined use of S100P or S100A2 with keratins enables easy to distinction between secretory coils and ducts.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Boni R, Burg G, Doguoglu A et al (1997) Immunohistochemical localization of the Ca2+ binding S100 proteins in normal human skin and melanocytic lesions. Br J Dermatol 137:39–43
Eckert RL, Broome AM, Ruse M, Robinson N, Ryan D, Lee K (2004) S100 proteins in the epidermis. J Invest Dermatol 123:23–33
Langbein L, Rogers MA, Praetzel S et al (2005) Characterization of a novel human type II epithelial keratin K1b, specifically expressed in eccrine sweat glands. J Invest Dermatol 125:428–444
Langbein L, Reichelt J, Eckhart L et al (2013) New facets of keratin K77: interspecies variations of expression and different intracellular location in embryonic and adult skin of humans and mice. Cell Tissue Res 354:793–812
Li H, Li X, Zhang M et al (2015a) Three-dimensional co-culture of BM-MSCs and eccrine sweat gland cells in Matrigel promotes transdifferentiation of BM-MSCs. J Mol Histol 46:431–438
Li H, Zhang X, Zeng S et al (2015b) Combination of keratins and alpha-smooth muscle actin distinguishes secretory coils from ducts of eccrine sweat glands. Acta Histochem 117:275–278
Li H, Chen L, Zhang M, Zhang B (2016a) Foxa1 gene and protein in developing rat eccrine sweat glands. J Mol Histol
Li H, Zhang M, Chen L, Li X, Zhang B (2016b) Human eccrine sweat gland cells reconstitute polarized spheroids when subcutaneously implanted with Matrigel in nude mice. J Mol Histol 47:485–490
Li X, Li H, Zhang M, Chen L, Zhang B (2017) Cell proliferation and differentiation during the three dimensional reconstitution of eccrine sweat glands. J Mol Histol 1–8
Lu CP, Polak L, Rocha AS et al (2012) Identification of stem cell populations in sweat glands and ducts reveals roles in homeostasis and wound repair. Cell 150:136–150
Mitoma C, Kohda F, Mizote Y et al (2014) Localization of S100A2, S100A4, S100A6, S100A7, and S100P in the human hair follicle. Fukuoka Igaku Zasshi 105:148–156
Pontiggia L, Biedermann T, Bottcher-Haberzeth S et al (2014) De novo epidermal regeneration using human eccrine sweat gland cells: higher competence of secretory over absorptive cells. J Invest Dermatol 134:1735–1742
Rittie L, Sachs DL, Orringer JS, Voorhees JJ, Fisher GJ (2013) Eccrine sweat glands are major contributors to reepithelialization of human wounds. Am J Pathol 182:163–171
Saga K (2002) Structure and function of human sweat glands studied with histochemistry and cytochemistry. Prog Histochem Cytochem 37:323–386
Sato K, Sato F (1987) Nonisotonicity of simian eccrine primary sweat induced in vitro. Am J Physiol 252:R1099–R1105
Shrestha P, Muramatsu Y, Kudeken W et al (1998) Localization of Ca(2+)-binding S100 proteins in epithelial tumours of the skin. Virchows Arch 432:53–59
Zhu L, Okano S, Takahara M et al (2013) Expression of S100 protein family members in normal skin and sweat gland tumors. J Dermatol Sci 70:211–219
Acknowledgements
The manuscript was supported in part by the National Natural Science Foundation of China (81471882, 81071551, 81571905), and the Natural Science Foundation of Guangdong Province, China (2014A030313476).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
We declare we have no competing financial, personal or other relationships with other people or organizations.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Li, H., Zhang, M., Chen, L. et al. Expression of S100A2 and S100P in human eccrine sweat glands and their application in differentiating secretory coil-like from duct-like structures in the 3D reconstituted eccrine sweat spheroids. J Mol Hist 48, 219–223 (2017). https://doi.org/10.1007/s10735-017-9721-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10735-017-9721-8