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Overexpression of PLAP-1 in bone marrow stromal cells inhibits the rat critical-size skull defect repair

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Abstract

Periodontal ligament-associated protein-1 (PLAP-1) is an osteogenisis negative regulatory gene that inhibits the differentiation of rat bone marrow stromal cells (rBMSCs) into osteoblasts in vitro. The aim of this study was to verify whether PLAP-1 acted as an inhibitory factor of rBMSCs in rat critical-size skull defect repair in vivo. Six-week old male Wistar rats treated with a drill-hole injury were randomly assigned into five groups PLAP-1-transfected rBMSC group, empty vector-transfected rBMSC group, normal rBMSC group, collagen group and blank control group according to the treatment factors. Skull specimens were obtained at 8 weeks after surgery. X-ray examination, histological observation of hard tissue slices (HE, Masson staining and von Kossa staining), immunohistochemical staining were applied to evaluate the repair effects. X-ray examination showed that repair effect of the PLAP-1-transfected rBMSC group was significantly worse than that of the empty vector-transfected rBMSC group and normal rBMSC group. In HE staining, regenerated bone could only be observed in the partial defect area of the PLAP-1-transfected rBMSC group. However, new bone filled nearly the entire defects in the empty vector-transfected rBMSC group and normal rBMSC group. Mineralization of new bone in the two groups was significantly higher than that of the PLAP-1-transfected rBMSC group, according to Masson and von Kossa staining. Meanwhile, the transfected PLAP-1 gene worked well in vivo. Positive expression of PLAP-1 protein was only distributed in the newly formed bone of the PLAP-1-transfected rBMSC group, no positive staining was observed in the other four groups. Overexpression of PLAP-1 in rBMSCs inhibits new bone formation and mineralization in rat critical-size skull defects in vivo.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (81271138). Open Foundation of Shandong Provincial Key Laboratory of Oral Biomedicine (SDKQ201403).

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The authors declare no conflict of interest.

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    Authors and Affiliations

    1. Shandong Provincial Key Laboratory of Oral Biomedicine, Department of Periodontology, School and Hospital of Stomatology, Shandong University, 44-1# West Wenhua Road, Jinan, 250012, Shandong, People’s Republic of China

      Xijiao Yu, Jing Sun, Changjie Xiao, Shuang Liu & Shu Li

    2. Department of Endodontics, Jinan Stomatological Hospital, 101# Jingliu Road, Jinan, 250001, Shandong, People’s Republic of China

      Xijiao Yu & Ying Hu

    3. School of Stomatology, Binzhou Medical University, 522#, Huanghe Sanlu, Bincheng District, Bincheng City, 256603, Shandong, People’s Republic of China

      Yan Gao

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    1. Xijiao Yu
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    Correspondence to Shu Li.

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    Xijiao Yu and Jing Sun have contributed equally to this work.

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    Yu, X., Sun, J., Hu, Y. et al. Overexpression of PLAP-1 in bone marrow stromal cells inhibits the rat critical-size skull defect repair. J Mol Hist 46, 251–261 (2015). https://doi.org/10.1007/s10735-015-9623-6

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    • DOI: https://doi.org/10.1007/s10735-015-9623-6

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