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Increased expression of BAG-1 in rat brain cortex after traumatic brain injury

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Abstract

BAG-1 protein was initially identified as a Bcl-2-binding protein. It was reported to enhance Bcl-2 protection from cell death, suggesting that BAG-1 represents a new type of anti-cell death gene. Moreover, recent study has shown that BAG-1 can enhance the proliferation of neuronal precursor cells, attenuate the growth inhibition induced by siah1. However, its function and expression in the central nervous system lesion are not been understood very well. In this study, we performed a traumatic brain injury (TBI) model in adult rats and investigated the dynamic changes of BAG-1 expression in the brain cortex. Double immunofluorescence staining revealed that BAG-1 was co-expressed with NEURON and glial fibrillary acidic protein (GFAP). In addition, we detected that proliferating cell nuclear antigen had the co-localization with GFAP, and BAG-1. All our findings suggested that BAG-1 might involve in the pathophysiology of brain after TBI.

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Abbreviations

BAG-1:

Bcl-2-associated athanogene-1

CNS:

Central nervous system

PAGE:

Polyacrylamide gel electrophoresis

ECL:

Enhanced chemiluminescence system

BSA:

Bovine serum albumin

NeuN:

Neuronal nuclei

GFAP:

Glial fibrillary acidic protein

CASPASE-3:

Cysteinyl aspartate-specific protease 3

PCNA:

Proliferating cell nuclear antigen

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31071228 and No. 30670648); Nature Science Foundation of Jiangsu Colleges and Universities Grant (09KJB320011); A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. The Jiangsu Province Key Laboratory of Neuroregeneration, Nantong University, Nantong, 226001, Peoples Republic of China

    Ting Xu, Xueqian Wang, Maohong Cao, Xinmin Wu, Yaohua Yan, Hongran Fu, Wei Zhao, Peipei Gong, Kaifu Ke & Xingxing Gu

  2. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Peoples Republic of China

    Maohong Cao, Xinmin Wu, Yaohua Yan, Hongran Fu & Kaifu Ke

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  1. Ting Xu
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  2. Xueqian Wang
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  3. Maohong Cao
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  4. Xinmin Wu
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  7. Wei Zhao
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  9. Kaifu Ke
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  10. Xingxing Gu
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Correspondence to Kaifu Ke or Xingxing Gu.

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Xu, T., Wang, X., Cao, M. et al. Increased expression of BAG-1 in rat brain cortex after traumatic brain injury. J Mol Hist 43, 335–342 (2012). https://doi.org/10.1007/s10735-012-9408-0

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