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Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation

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Summary

The present study aimed to clarify how myoepithelial cells behave during regeneration of an atrophied sublingual gland by investigating cell proliferation and ultrastructure. Atrophy of rat sublingual glands was induced by unilateral ligation of the excretory duct near the hilum with metal clips, which were then removed after one week of ligation for regeneration. The sublingual glands 0–14 days after unligation were examined with single immunohistochemistry for actin as a marker of myoepithelial cells, double immunohistochemistry for actin and proliferating cell nuclear antigen (PCNA) as a marker of proliferating cells, and transmission electron microscopy (TEM). The single immunohistochemistry and TEM showed that myoepithelial cells surrounded residual ducts in the atrophied glands and immature and mature acini in the regenerating glands. Although PCNA-positive myoepithelial cells were identified during regeneration, PCNA labeling indices of myoepithelial cells were low at all time points except at day 7. Ultrastructurally, myoepithelial cells showing bizarre shaped structures in the atrophy changed with maturation of differentiating acinar cells and appeared normal in the regenerated glands. There was no differentiation of the remaining duct cells to myoepithelial cells. These observations suggest that proliferation of myoepithelial cells and differentiation to myoepithelial cells do not commonly participate in the regeneration of atrophied sublingual glands and that the bizarre shaped myoepithelial cells in the atrophied sublingual glands recover the original shapes with acinar cell regeneration.

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References

  • Batsakis JG, Kraemer B, Sciubba JJ (1983) The pathology of head and neck tumors: The myoepithelial cell and its participation in salivary gland neoplasia. Part17. Head Neck Surg 5:222–233

    Article  PubMed  CAS  Google Scholar 

  • Batsakis JG, Regezi JA, Luna MA, El-Naggar A (1989) Histogenesis of salivary gland neoplasms: a postulate with prognostic implications. J Laryngol Otol 103:939–944

    Article  PubMed  CAS  Google Scholar 

  • Burford-Mason AP, Cummins MM, Brown DH, MacKay AJ, Dardick I (1993) Immunohistochemical analysis of the proliferative capacity of duct and acinar cells during ligation-induced atrophy and subsequent regeneration of rat parotid gland. J Oral Pathol Med 22:440–446

    Article  PubMed  CAS  Google Scholar 

  • Burgess KL, Dardick I, Cummins MM, Burford-Mason AP (1996) Myoepithelial cells actively proliferate during atrophy and regeneration of rat parotid gland. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 82:674–680

    Article  PubMed  CAS  Google Scholar 

  • Burgess KL, Dardick I (1998) Cell population changes during atrophy and regeneration of rat parotid gland. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:699–706

    Article  PubMed  CAS  Google Scholar 

  • Cummins M, Dardick I, Brown D, Burford-Mason A (1994) Obstructive sialadenitis: a rat model. J Otolaryngol 23:50–56

    PubMed  CAS  Google Scholar 

  • Cutler LS, Chaudhry AP (1973) Differentiation of the myoepithelial cells of the rat submandibular gland in vivo and in vitro: an ultrastructural study. J Morphol 140:343–354

    Article  PubMed  CAS  Google Scholar 

  • Dardick I, Burford-Mason AP (1993) Current status of histogenetic and morphogenetic concepts of salivary gland tumorigenesis. Crit Rev Oral Biol 4:639–667

    CAS  Google Scholar 

  • Dardick I, Nostrand AWP (1987) Morphogenesis of salivary gland tumors: a prerequisite to improving classification. Pathol Ann 22:1–53

    Google Scholar 

  • Emmelin N, Garrett JR, Ohlin P (1974) Secretory activity and the myoepithelial cells of salivary glands after duct ligation in cats. Arch Oral Biol 19:275–283

    Article  PubMed  CAS  Google Scholar 

  • Garrett JR, Emmelin N (1979) Activities of salivary myoepithelial cells: a review. Med Biol 57:1–28

    PubMed  CAS  Google Scholar 

  • Hanks CT, Chaudhry AP (1971) Regeneration of rat submandibular gland following partial extirpation. A light and electron microscopic study. Am J Anat 130:195–208

    Article  PubMed  CAS  Google Scholar 

  • Harrison JD, Garrett JR (1972) Mucocele formation in cats by glandular duct ligation. Arch Oral Biol 17:1403–1414

    Article  PubMed  CAS  Google Scholar 

  • Harrison JD, Badir MS (1998) Chronic submandibular sialadenitis: ultrastructure and phosphate histochemistry. Ultrastruct Pathol 22:431–437

    Article  PubMed  CAS  Google Scholar 

  • Harrison JD, Fouad HMA, Garrett JR (2001) Variation in the response to ductal obstruction of feline submandibular and sublingual salivary glands and the importance of the innervation. J Oral Pathol Med 30:29–34

    Article  PubMed  CAS  Google Scholar 

  • Ihrler S, Blasenbreu-Vogt S, Sendelhofert A, Rössle M, Harrison JD, Löhrs U (2004) Regeneration in chronic sialadenitis: an analysis of proliferation and apoptosis based on double immunohistochemical labelling. Virchows Arch 444:356–361

    Article  PubMed  Google Scholar 

  • McCormick D, Hall PA (1992) The complexities of proliferating cell nuclear antigen. Histopathol 21:591–594

    Article  CAS  Google Scholar 

  • Miyachi K, Fritzler MJ, Tan EM (1978) Autoantibody to a nuclear antigen in proliferating cells. J Immunol 121:2228–2234

    PubMed  CAS  Google Scholar 

  • Norberg L, Dardick I, Leung R, Burford-Mason AP, Rippstein P (1992) Immunogold localization of actin and cytokeratin filaments in myoepithelium of human parotid salivary gland. Ultrastruct Pathol 16:555–568

    Article  PubMed  CAS  Google Scholar 

  • Radnor CJP (1972) Myoepithelium in involuting mammary glands of the rat. J Anat 112:355–365

    PubMed  CAS  Google Scholar 

  • Redman RS, Ball WD (1979) Diffrenetiation of myoepithelial cells in the developing rat sublingual gland. Am J Anat 156:543–566

    Article  PubMed  CAS  Google Scholar 

  • Redman RS, Sweney LR, Mclaughlin ST (1980) Differentiation of myoepithelial cells in the developing rat parotid gland. Am J Anat 158:299–320

    Article  PubMed  CAS  Google Scholar 

  • Redman RS (1994) Myoepithelium of salivary glands. Microsc Res Tech 27:25–45

    Article  PubMed  CAS  Google Scholar 

  • Scott J, Liu P, Smith PM (1999) Morphological and functional characteristics of acinar atrophy and recovery in the duct-ligated parotid gland of the rat. J Dent Res 78:1711–1719

    Article  PubMed  CAS  Google Scholar 

  • Takahashi S, Domon T, Yamamoto T, Wakita M (1997) Regeneration of myoepithelial cells in rat submandibular glands after yttrium aluminium garnett laser irradiation. Int J Exp Pathol 78:91–99

    Article  PubMed  CAS  Google Scholar 

  • Takahashi S, Nakamura S, Suzuki R, Islam N, Domon T, Yamamoto T, Wakita M (2000) Apoptosis and mitosis of parenchymal cells in the duct-ligated rat submandibular gland. Tissue Cell 32:457–463

    Article  PubMed  CAS  Google Scholar 

  • Takahashi S, Nakamura S, Shinzato K, Domon T, Yamamoto T, Wakita M (2001) Apoptosis and proliferation of myoepithelial cells in atrophic rat submandibular glands. J Histochem Cytochem 49:1557–1563

    PubMed  CAS  Google Scholar 

  • Takahashi S, Shinzato K, Nakamura S, Domon T, Yamamoto T, Wakita M (2002) The roles of apoptosis and mitosis in atrophy of the rat sublingual gland. Tissue Cell 34:297–304

    Article  PubMed  CAS  Google Scholar 

  • Takahashi S, Shinzato K, Domon T, Yamamoto T, Wakita M (2003) Proliferation and distribution of myoepithelial cells during atrophy of the rat sublingual gland. J Oral Pathol Med 32:90–94

    Article  PubMed  CAS  Google Scholar 

  • Takahashi S, Shinzato K, Nakamura S, Domon T, Yamamoto T, Wakita M (2004a) Cell death and cell proliferation in the regeneration of atrophied rat submandibular glands after duct ligation. J Oral Pathol Med 33:23–29

    Article  Google Scholar 

  • Takahashi S, Shinzato K, Domon T, Yamamoto T, Wakita M (2004b) Mitotic proliferation of myoepithelial cells during regeneration of atrophied rat submandibular glands after duct ligation. J Oral Pathol Med 33:430–434

    Article  CAS  Google Scholar 

  • Takahashi S, Nakamura S, Domon T, Yamamoto T, Wakita M (2005) Active participation of apoptosis and mitosis in sublingual gland regeneration of the rat following release from duct ligation. J Mol Histol 36:199–205

    Article  PubMed  CAS  Google Scholar 

  • Tandler B, Denning CR, Mandel ID, Kutscher AH (1970) Ultrastructure of human labial glands; III. myoepithelium and ducts. J Morphol 130:227–246

    Article  PubMed  CAS  Google Scholar 

  • Walker NI, Benett RE, Kerr JFK (1989) Cell death by apoptosis during involution of the lactating breast in mice and rats. Am J Anat 185:19–32

    Article  PubMed  CAS  Google Scholar 

  • Walker NI, Gobé GC (1987) Cell death and cell proliferation during atrophy of the rat parotid gland induced by duct obstruction. J Pathol 153:333–344

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This study was supported by Grant for Scientific Research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (No.␣17390499).

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Authors and Affiliations

  1. Department of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, 060-8586, Japan

    S. Takahashi, A. K. S. Arambawatta, T. Domon, T. Yamamoto & M. Wakita

  2. Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, 060-8586, Japan

    T. Kohgo

  3. Department of Oral Physiology, Showa University School of Dentistry, Tokyo, 142-8555, Japan

    S. Nakamura

Authors
  1. S. Takahashi
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  2. T. Kohgo
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  3. S. Nakamura
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  4. A. K. S. Arambawatta
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  5. T. Domon
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  6. T. Yamamoto
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  7. M. Wakita
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Correspondence to S. Takahashi.

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Takahashi, S., Kohgo, T., Nakamura, S. et al. Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation. J Mol Hist 36, 373–379 (2005). https://doi.org/10.1007/s10735-005-9009-2

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  • DOI: https://doi.org/10.1007/s10735-005-9009-2

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