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Ultraviolet A-specific induction of anthocyanin biosynthesis and PAL expression in tomato (Solanum lycopersicum L.)

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Abstract

Ultraviolet A (UV-A) is an environmental stimulus, and UV-A-induced anthocyanin biosynthesis has been previously investigated in the tomato plant (Solanum lycopersicum L.). UV-A induced anthocyanin biosynthesis in tomato seedlings and co-irradiation with visible light and UV-A did not influence the content of UV-A-induced anthocyanin accumulation. UV-A irradiation induced significant accumulations of anthocyanin in both the cotyledons and hypocotyls of tomato seedlings. Anthocyanin production increased gradually in the tomato hypocotyls after exposure to UV-A and reached maximum levels at 12 h. In the cotyledons, anthocyanin accumulation was significantly increased at 1 h after UV-A exposure and was reduced afterward; however, it increased again beginning at 3 h. The expression of the phenylalanine ammonia-lyase (SlPAL5) gene was shown to be increased after UV-A exposure in a time-dependent manner. UV-A irradiation was also shown to induce anthocyanin accumulation in the epidermis of the tomato fruit; however, SlPAL5 transcripts were detected only at 3 and 24 h after UV-A treatment. After a 1 h pulse of UV-A, SlPAL5 transcripts were increased significantly in tomato cotyledons and hypocotyls after transfer to dark conditions for a short time.

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References

  • Ahmad M, Cashmore AR (1997) The blue-light receptor cryptochrome 1 shows functional dependence on phytochrome A or phytochrome B in Arabidopsis thaliana. Plant J 11:421–427

    Article  CAS  PubMed  Google Scholar 

  • Bouché N, Fait A, Bouchez D, Møller SG, Fromm H (2003) Mitochondrial succinic-semialdehyde dehydrogenase of the c-aminobutyrate shunt is required to restrict levels of reactive oxygen intermediates in plants. Proc Natl Acad Sci USA 100:6843–6848

    Article  PubMed  Google Scholar 

  • Briggs WR, Christie JM (2002) Phototropins 1 and 2: versatile plant blue-light receptors. Trends Plant Sci 7:204–210

    Article  CAS  PubMed  Google Scholar 

  • Briggs WR, Olney MA (2001) Photoreceptors in plant photomorphogenesis to date. Five phytochromes, two cryptochromes, one phototropin, and one superchrome. Plant Physiol 125:85–88

    Article  CAS  PubMed  Google Scholar 

  • Brődenfeldt R, Mohr H (1988) Time courses for phytochrome-induced enzyme levels in phenylpropanoid metabolism (phenylalanine ammonia-lyase, naringenin-chalcone synthase) compared with time courses for phytochrome-mediated end-product accumulation (anthocyanin, quercetin). Planta 41:383–390

    Article  Google Scholar 

  • Campos-Vargas R, Saltveit ME (2002) Involvement of putative chemical wound signals in the induction of phenolic metabolism in wounded lettuce. Physiol Plant 114:73–84

    Article  CAS  PubMed  Google Scholar 

  • Campos-Vargas R, Nonogaki H, Suslow T, Saltveit ME (2005) Heat shock treatments delay the increase in wound induced phenylalanine ammonia-ammonia-lyase activity by altering its expression, not its induction in Romaine lettuce (Lactuca sativa) tissue. Physiol Plant 132:82–91

    Article  Google Scholar 

  • Chen DQ, Li ZY, Pan RC, Wang XJ (2006a) Anthocyanin accumulation mediated by blue light and cytokinin in Arabidopsis seedlings. J Integr Plant Biol 48:420–425

    Article  CAS  Google Scholar 

  • Chen JY, Wen PF, Kong WF, Pan QH, Zhan JC, Li JM, Wan SB, Huang WD (2006b) Effect of salicylic acid on phenylpropanoids and phenylalanine ammonia-lyase in harvested grape berries. Postharvest Biol Technol 40:64–72

    Article  CAS  Google Scholar 

  • Chen JY, He LH, Jiang YM, Wang Y, Joyce DC, Ji Z (2008) Role of phenylalanine ammonia-lyase in heat pretreatment-induced chilling tolerance in banana fruit. Physiol Plant 132:318–328

    Article  CAS  PubMed  Google Scholar 

  • Christie JM, Jenkins GI (1996) Distinct UV-B and UV-A/blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells. Plant Cell 8:1555–1567

    Article  CAS  PubMed  Google Scholar 

  • Feinbaum Rl, Storz G, Ausubel FM (1991) High intensity and blue light regulated expression of chimeric chalcone synthase genes in transgenic Arabidopsis thaliana plants. Mol Gen Genet 226:449–456

    Article  CAS  PubMed  Google Scholar 

  • González-Gallego J, Sánchez-Campos S, Tuñón MJ (2007) Anti-inflammatory properties of dietary flavonoids. Nutr Hosp 22:287–293

    PubMed  Google Scholar 

  • Guo J, Wang MH (2009) Characterization of the phenylalanine ammonia-lyase gene (SlPAL5) from tomato (Solanum lycopersicum L.). Mol Biol Rep 36:1579–1585

    Article  CAS  PubMed  Google Scholar 

  • Hahlbrock K, Grisebach H (1979) Enzymic controls in the biosynthesis of lignin and flavonoids. Annu Rev Plant Physiol 30:105–130

    Article  CAS  Google Scholar 

  • Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol 40:347–369

    Article  CAS  Google Scholar 

  • Hauser BA, Cordonnier-Pratt MM, DanieI-Vedele F, Pratt LH (1995) The phytochrome gene family in tomato includes a novel subfamily. Plant Mol Biol 29:1143–1155

    Article  CAS  PubMed  Google Scholar 

  • Hirner AA, Veit S, Seitz HU (2001) Regulation of anthocyanin biosynthesis in UV-A-irradiated cell cultures of carrot and in organs of intact carrot plants. Plant Sci 161:315–322

    Article  CAS  PubMed  Google Scholar 

  • Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7:1071–1083

    Article  CAS  PubMed  Google Scholar 

  • Huub L, Kerckhoffs J, Kendrick RE (1997) Photocontrol of anthocyanin biosynthesis in tomato. J Plant Res 110:141–149

    Article  Google Scholar 

  • Jenkins GI, Long JC, Wade HK, Shenton MR, Bibikova TN (2001) UV and blue light signaling: pathways regulating chalcone synthase gene expression in Arabidopsis. New Phytol 151:121–131

    Article  CAS  Google Scholar 

  • Kashiwagi I, Kobayashi Y, Matsukawa T (1977) Effect of ultraviolet rays on the flower color of Primula malacoides I. Discoloration of flower color under a covering of plastic film absorbing ultraviolet rays. J Jpn Soc Hortic Sci 46:66–71

    Article  Google Scholar 

  • Kataoka I, Sugiyama A, Beppu K (2003) Role of ultraviolet radiation in accumulation of anthocyanin in berries of ‘Gros Colman’ grapes (Vitis vinifera L.). J Jpn Soc Hortic Sci 72:1–6

    Article  CAS  Google Scholar 

  • Krauss P, Markstädter C, Riederer M (1997) Attenuation of UV radiation by plant cuticles from woody species. Plant Cell Environ 20:1079–1085

    Article  Google Scholar 

  • Kumar A, Ellis BE (2001) The phenylalanine ammonia-lyase gene family in raspberry: structure, expression, and evolution. Plant Physiol 127:230–239

    Article  CAS  PubMed  Google Scholar 

  • Lafuente MT, Zacarias L, Martinez-Telez MA, Sanchez-Ballesta MT, Granell A (2003) Phenylalanine ammonia-lyase and ethylene in relation to chilling injury as affected by fruit age in citrus. Postharvest Biol Technol 29:308–317

    Article  Google Scholar 

  • Lee SW, Robb J, Nazar RN (1992) Truncated phenylalanine ammonia-lyase expression in tomato (Lycopersicon esculentum). J Biol Chem 267:11824–11830

    CAS  PubMed  Google Scholar 

  • Matsumaru K, Kamihama T, Inada K (1971) Covering materials with different transmission properties on anthocyanin content of eggplant pericarp. Environ Control Biol 9:91–97

    Google Scholar 

  • McLennan AG (1987) DNA damage, repair, and mutagenesis. In: Bryant JA, Dunham VL (eds) DNA replication in plants. CRC Press, Boca Raton, pp 135–186

    Google Scholar 

  • Mol J, Jenkins GI, Schafer E, Weiss D (1996) Signal perception, transduction and gene expression involved in anthocyanin biosynthesis. Crit Rev Plant Sci 15:525–557

    CAS  Google Scholar 

  • Muir SR, Collins GJ, Robinson S, Hughes S, Bovy A, Ric-De-Vos CH, van-Tunen AJ, Verhoeyen ME (2001) Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nat Biotechnol 19:470–474

    Article  CAS  PubMed  Google Scholar 

  • O’Neill SD, Tong Y, Spörlein B, Forkmann G, Yoder JI (1990) Molecular genetic analysis of chalcone synthase in Lycopersicon esculentum and an anthocyanin-deficient mutant. Mol Gen Genet 224:279–288

    Article  PubMed  Google Scholar 

  • Quail PH (1994) Phytochrome genes and their expression. In: Kendrick RE, Kronenberg GHM (eds) Photomorphogenesis in plants. Kluwer, London, pp 71–104

    Google Scholar 

  • Reddy VS, Goud KV, Sharma R, Reddy AR (1994) UV-B responsive anthocyanin production in a rice cultivar is associated with a specific phase of phenylalanine ammonia lyase biosynthesis. Plant Physiol 105:1059–1066

    CAS  PubMed  Google Scholar 

  • Ryder TB, Hedrick SA, Bell JN, Liang X, Clouse SD, Lamb CJ (1987) Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris. Mol Gen Genet 210:219–233

    Article  CAS  PubMed  Google Scholar 

  • Sapir M, Oren-Shamir M, Ovadia R, Reuveni M, Evenor D, Tadmor Y, Nahon S, Shlomo H, Chen L, Meir A, Levin I (2008) Molecular aspects of anthocyanin fruit tomato in relation to high pigment-1. J Hered 99:292–303

    Article  CAS  PubMed  Google Scholar 

  • Saunders JA, McClure JW (1975) Phytochrome controlled phenylalanine ammonia lyase activity in Hordeum vulgare plastids. Phytochemistry 14:1285–1289

    Article  CAS  Google Scholar 

  • Singh A, Selvi MT, Sharma R (1999) Sunlight-induced anthocyanin pigmentation in maize vegetative tissues. J Exp Bot 50:1619–1625

    Article  CAS  Google Scholar 

  • Sinha RP, Häder DP (2002) UV-induced DNA damage and repair: a review. Photochem Photobiol Sci 1:225–236

    Article  CAS  PubMed  Google Scholar 

  • Sinha RP, Klisch M, Gröniger A, Häder DP (1998) Ultraviolet-absorbing/screening substances in cyanobacteria, phytoplankton and macroalgae. J Photochem Photobiol 47:83–94

    Article  CAS  Google Scholar 

  • Solovchenko A, Schmitz-Eiberger M (2003) Significance of skin flavonoids for UV-B protection in apple fruits. J Exp Bot 54:1977–1984

    Article  CAS  PubMed  Google Scholar 

  • Sreelakshmi Y, Sharma R (2008) Differential regulation of phenylalanine ammonia lyase activity and protein level by light in tomato seedlings. Plant Physiol Biochem 46:444–451

    Article  CAS  PubMed  Google Scholar 

  • Tahara S (2007) A journey of twenty-five years through the ecological biochemistry of flavonoids. Biosci Biotechnol Biochem 71:1387–1404

    Article  CAS  PubMed  Google Scholar 

  • Taylor RM, Tobin AK, Bray CM (1997) DNA damage and repair in plants. In: Lumsden PL (ed) Plants and UV-B: responses to environmental change. Cambridge University Press, Cambriage, pp 53–75

    Google Scholar 

  • Toguri T, Umemoto N, Kobayashi O, Ohtani T (1993) Activation of anthocyanin synthesis genes by white light in eggplant hypocotyl tissues, and identification of an inducible P-450 cDNA. Plant Mol Biol 23:933–946

    Article  CAS  PubMed  Google Scholar 

  • Ververidis F, Trantas E, Douglas C, Vollmer G, Kretzschmar G, Panopoulos N (2007) Biotechnology of flavonoids and other phenylpropanoid-derived natural products. Part I: Chemical diversity, impacts on plant biology and human health. Biotechnol J 10:1214–1234

    Article  Google Scholar 

  • Weisshaar B, Jenkins GI (1998) Phenylpropanoid biosynthesis and its regulation. Curr Opin Plant Biol 1:251–257

    Article  CAS  PubMed  Google Scholar 

  • Xia Q, Yin JJ, Fu PP, Boudreau MD (2007) Photo-irradiation of Aloe vera by UVA-formation of free radicals, singlet oxygen, superoxide, and induction of lipid peroxidation. Toxicol Lett 168:165–175

    Article  CAS  PubMed  Google Scholar 

  • Xiong FS, Day TA (2001) Effects of solar ultraviolet-B radiation during springtime ozone depletion on photosynthesis and biomass production of Antarctic vascular plants. Plant Physiol 125:738–751

    Article  CAS  PubMed  Google Scholar 

  • Yarosh DB, Smiles KA (2009) DNA repair and photoprotection. In: Lim HW, Draelos ZD (eds) Clinical guide to sunscreens and photoprotection. Inform Healthcare, New York, pp 169–197

    Google Scholar 

  • Zhou B, Li Y, Xu Z, Yan H, Homma S, Kawabata S (2007) Ultraviolet A-specific induction of anthocyanin biosynthesis in the swollen hypocotyls of turnip (Brassica rapa). J Exp Bot 58:1771–1781

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the Research Institute of Bioscience & Biotechnology, Kangwon National University.

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  1. School of Biotechnology, Kangwon National University, Chuncheon, Kangwon-do, 200-701, South Korea

    Jia Guo & Myeong-Hyeon Wang

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  1. Jia Guo
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Correspondence to Myeong-Hyeon Wang.

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Guo, J., Wang, MH. Ultraviolet A-specific induction of anthocyanin biosynthesis and PAL expression in tomato (Solanum lycopersicum L.). Plant Growth Regul 62, 1–8 (2010). https://doi.org/10.1007/s10725-010-9472-y

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