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Is there crosstalk between extracellular and intracellular calcium mobilization in jasmonic acid signaling

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Abstract

The changes in cytosolic Ca2+ levels play an important role in the jasmonic acid (JA) signal transduction pathway. We demonstrate that an increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) of Arabidopsis leaf cells was affected by pretreatment with heparin and 3,4,5-trimethoxybenzoic acid 8-(diethylamino)octyl ester (TMB-8). With pretreatment of heparin, an antagonist of inositol 1,4,5-trisphosphate (IP3) sensitive channels, the basal and JA induced fluorescence of [Ca2+]cyt were both decreased. Furthermore, heparin and TMB-8, another antagonist of IP3 sensitive channels, enhanced the JA-induced gene expression of JR1. These data suggest that there may be a fine tune control system between extracellular and intracellular Ca2+ mobilization in JA signaling pathway.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (grant no. 30700428), Beijing Natural Science Foundation (grant no. 5072009) and The New Star Plan of Science and Technology item of Beijing (grant no. 2006B26).

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Authors and Affiliations

  1. Department of Biotechnology, Beijing Agriculture College, Beijing, 102206, People’s Republic of China

    Qing-peng Sun, Shan-xia Wan, Fu-kuan Zhao & Yu-lan Hao

  2. Center of Tissue Culture, Beijing Agriculture College, Beijing, 102206, People’s Republic of China

    Yong-kun Yu

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  1. Qing-peng Sun
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Correspondence to Qing-peng Sun.

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Sun, Qp., Yu, Yk., Wan, Sx. et al. Is there crosstalk between extracellular and intracellular calcium mobilization in jasmonic acid signaling. Plant Growth Regul 57, 7–13 (2009). https://doi.org/10.1007/s10725-008-9317-0

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  • DOI: https://doi.org/10.1007/s10725-008-9317-0

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