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Comparative Efficacy of Abscisic Acid and Methyl Jasmonate for Indirect Somatic Embryogenesis in Medicago sativa L.

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Abstract

The effects of methyl jasmonate (MeJA) in relation to abscisic acid (ABA) on different phases of somatic embryogenesis were studied in Medicago sativa L. Different concentrations of both the growth inhibitors (0.0, 0.5, 5.0, 50.0 and 500.0 μM) were tested in five distinct phases of somatic embryogenesis, viz., induction, proliferation, differentiation, maturation and regeneration. Like ABA, MeJA also inhibited callus induction, callus growth, proliferation of embryogenic suspension as well as germination and conversion of somatic embryos. However, its inhibitory effects on various phases of somatic embryogenesis were less pronounced as compared to that due to ABA. In contrast to ABA, MeJA did not have any significant influence on the development of somatic embryos when applied in the differentiation phase. The study showed that ABA used routinely as an inducer of somatic embryo maturation in M. sativa could not be replaced by MeJA.

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Abbreviations

ABA:

abscisic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

JA:

jasmonic acid

MeJA:

methyl jasmonate

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  1. Department of Plant Physiology and Biotechnology, Szczecin University, Wąska 13, 71-415, Szczecin, Poland

    Izabela Ruduś, Ewa Kępczyńska & Jan Kępczyński

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  1. Izabela Ruduś
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  2. Ewa Kępczyńska
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Correspondence to Izabela Ruduś.

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Ruduś, I., Kępczyńska, E. & Kępczyński, J. Comparative Efficacy of Abscisic Acid and Methyl Jasmonate for Indirect Somatic Embryogenesis in Medicago sativa L.. Plant Growth Regul 48, 1–11 (2006). https://doi.org/10.1007/s10725-005-5136-8

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  • DOI: https://doi.org/10.1007/s10725-005-5136-8

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