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Genetic diversity assessment of Bulgarian durum wheat (Triticum durum Desf.) landraces and modern cultivars using microsatellite markers

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Abstract

The genetic diversity in a Triticum durum Desf. collection, consisting of 102 Bulgarian landraces, nine Bulgarian and 25 introduced cultivars was studied using 14 highly polymorphic microsatellite markers. A total of 100 alleles were identified, with an average of 7.14 alleles per marker. The gene diversity values (He) of the markers for the total samples ranged from 0.23 (WMS357 and WMS631) to 0.77 (WMS46), with an average of 0.52. Within the landraces that were collected from 18 sites in Southern Bulgaria showed 2–11 alleles per locus with an average of 6.07. The microsatellite analysis suggests that the genetic diversity among landraces is lower compared to the diversity levels for durum wheat in countries close to the main centers of wheat domestication. Breeding activities have caused significant reduction of the allelic polymorphism, elimination of rare alleles, and increase in the number of common alleles and the frequency of dominating alleles.

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Acknowledgments

The investigation was supported by the Bulgarian NCSR, contract CC-1415/04. The technical assistance of Mrs K. Prokopova is greatly acknowledged.

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Authors and Affiliations

  1. Institute of Genetics “Acad. Doncho Kostoff”, Bulgarian Academy of Sciences, Bulgaria Tzarigradsko shosse, 13 km, Sofia, 1113, Bulgaria

    Ganka Ganeva & Svetlana Landjeva

  2. KWS LOCHOW GMBH, Grimsehl str. 31, 37574, Einbeck, Germany

    Victor Korzun

  3. Institute for Plant Genetic Resources “K.Malkov”, Sadovo, 4122, Bulgaria

    Zaprjanka Popova

  4. AgroBioInstitute, 8 Dragan Tsankov Blvd., Sofia, 1164, Bulgaria

    Nikolai K. Christov

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  1. Ganka Ganeva
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  2. Victor Korzun
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  4. Zaprjanka Popova
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  5. Nikolai K. Christov
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Correspondence to Nikolai K. Christov.

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Ganeva, G., Korzun, V., Landjeva, S. et al. Genetic diversity assessment of Bulgarian durum wheat (Triticum durum Desf.) landraces and modern cultivars using microsatellite markers. Genet Resour Crop Evol 57, 273–285 (2010). https://doi.org/10.1007/s10722-009-9468-5

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