Skip to main content
SpringerLink
Log in

Promising genetic resources for resistance to wheat streak mosaic virus and the wheat curl mite in wheat-Thinopyrum partial amphiploids and their derivatives

  • Published:
Genetic Resources and Crop Evolution Aims and scope Submit manuscript

Abstract

Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite (WCM),Aceria tosichella Keifer, is one of the most destructive viral diseases of wheat found in many wheat producing areas of the world. Host resistance is the most effective method for controlling this disease and its vector. Symptomatological analysis and enzyme-linked immunosorbent assay (ELISA) were used to characterize WSMV-resistance in wheat-alien partial amphiploid lines and their derivatives. The results showed that most of partial amphiploids derived fromThinopyrum ponticum andTh. intermedium were free of systemic symptoms with very low ELISA readings that were similar to that of the non-inoculated Chinese Spring control. While the partial amphiploid lines 693 and PWM706 were identified as new genetic resources of resistance to WSMV. The present study demonstrated that both symptomatological and ELISA methods efficiently assessed WSMV-resistance in the wheat-alien hybrids and systemic symptom incidence and ELISA absorbance readings were highly correlated (r 2 = 0.8658−0.9323) over time following inoculation. The ELISA results also indicated that the virus did not buildup in the plant tissues of these virus-resistant partial amphiploids. Similar results were observed in chromosome translocation and substitution lines that have the geneWsm1 conferring WSMV resistance. However, the lines containing the geneWsm1 and all the partial amphiploid lines, except Agrotana, were susceptible to the WCM. One line derived from a cross of wheat and Agrotana, was effective in controlling the spread of WSMV and was highly resistant to the WCM. Another line and an accession ofTriticum dicoccoides (Koern.) Schweinf. were highly susceptible to WSMV and WCM. Early disease development was delayed in a new hard red winter cultivar McClintock. The partial WSMV-resistance of McClintock was demonstrated by initially low ELISA readings, and a lower percentage of infected plants than other WSMV-susceptible wheat. The use of the identified promising sources of resistance to WSMV and the WCM in wheat breeding is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andrews J.E. and Slykhuis J.T. 1956. Reaction of winter wheat varieties andTriticum ×Agropyron hybrids when inoculated with wheat streak mosaic virus by the mite vectorAceria tulipae Keifer. Plant Dis. Rep. 40: 513–516.

    Google Scholar 

  • Atkinson T.G. and Grant M.N. 1967. An evaluation of streak mosaic losses in winter wheat. Phytopathology 57: 188–192.

    Google Scholar 

  • Bockus W.W., Appel J.A., Bowden R.A., Fritz A.K., Gill B.S., Martin T.J., Sears R.G., Seifers D.L., Brown-Guedira G.L. and Eversmeyer M.G. 2001. Success stories: breeding for wheat disease resistance in Kansas. Plant Dis. 85: 453–461.

    Google Scholar 

  • Brûlé-Babel, 2001. Request for support for registration of UM5089. In: Graf R.L. and Fox S.L. (eds), Prairie Registration Recommending Committee for Grain: Wheat, Rye and Triticale Subcommittee Report 2001, Saskatoon, SK, pp. 105–110.

  • Cauderon Y. 1966. Etude cytogénétique de l’évolution du materiel issu de croisements entreTriticum aestivum etAgropyron intermedium. Annales Améliorament des Plantes 16: 43–70.

    Google Scholar 

  • Cauderon Y., Saigne B. and Dauge M. 1973. The resistance to wheat rusts ofAgropyron intermedium and its use in wheat inprovement. In: Sears E.R. and Sears L.M.S. (eds), Proceedings of the 4th International Wheat Genetics Symposium, University of Missouri, Columbia, Missouri. pp. 401–470.

    Google Scholar 

  • Chen Q., Conner R.L. and Laroche A. 1995. Identification of the parental chromosomes of the wheat-alien amphiploid Agrotana by genomicin situ hybridization. Genome 38: 1163–1169.

    PubMed  CAS  Google Scholar 

  • Chen Q., Conner R.L., Laroche A., Fedak G. and Thomas J.B. 1999. Genome origins ofThinopyrum chromosomes specifying resistance to wheat streak mosaic virus and its vector,Aceria tosichella. Genome 42: 289–295.

    Article  Google Scholar 

  • Chen Q., Conner R.L., Ahmad F., Laroche A., Fedak G. and Thomas J.B. 1998. Molecular characterization of the genome composition of partial amphiploids derived fromTriticum aestivum ×Thinopyrum ponticum andT. aestivum ×Th. intermedium as sources of resistance to wheat streak mosaic virus and its vector,Aceria tosichella. Theor. Appl. Genet. 97: 1–8.

    Article  CAS  Google Scholar 

  • Comeau A., Makkouk K.M., Ahmad F. and St-Pierre C.A. 1994. Bread wheat ×Agrotricum crosses as a source of immunity and resistance to the PAV strain of barley yellow dwarf luteovirus. Agronomie 2: 153–160.

    Google Scholar 

  • Conner R.L., Thomas J.B. and Whelan E.D.P. 1991. Comparison of WCM resistance for control wheat streak mosaic. Crop Sci. 31: 315–318.

    Article  Google Scholar 

  • Edwards M.C. and McMullen M.P. 1988. Variation in tolerance to wheat streak mosaic virus among cultivars of hard red spring wheat. Plant Dis. 72: 705–707.

    Google Scholar 

  • Fedak G., Chen Q., Conner R.L., Laroche A., Petroski R. and Armstrong K.W. 2000. Characterization of wheat-Thinopyrum partial amphiploids by meiotic analysis and genomicin situ hybridization. Genome 43: 712–719.

    Article  CAS  PubMed  Google Scholar 

  • Gill B.S., Friebe B., Wilson D.L. and Cox T.S. 1995. Registration of KS93WGRC27 wheat streak mosaic virus-resistant T4DL.4Ai#2S wheat germplasm. Crop Sci. 35: 1236–1237.

    Article  Google Scholar 

  • Harvey T.L., Seifers D.L., Martin T.J., Brown-Guedira G. and Gill B.S. 1999. Survival of wheat curl mites on different sources of resistance in wheat. Crop Sci. 39: 1887–1889.

    Article  Google Scholar 

  • Harvey T.L., Martin T.J. and Seifers D.L. 1994. Importance of plant resistance to insect and mite vectors in controlling virus diseases of plants: Resistance to the wheat curl mite (Acari: Eriophyidae). J. Agric. Entomol. 11: 271–277.

    Google Scholar 

  • Jedlinski H., Rochow W.F. and Brown C.M. 1977. Tolerance to barley yellow dwarf virus in oats. Phytopathology 67: 1408–1411.

    Google Scholar 

  • Larson R.I. and Atkinson T.G. 1973. Wheat-Agropyron chromosome substitution lines as sources of resistance to wheat streak mosaic virus and its vector,Aceria tulipae. In: Sears E.R. and Sears L.M.S. (eds), Proceedings of the 4th International Wheat Genetics Symposium, University of Missouri, Columbia, MO, pp. 173–177.

    Google Scholar 

  • Li H.J., Conner R.L., Chen Q., Jia X., Li H., Graf R.J., Laroche A. and Kuzyk A.D. 2003. Different reactions to wheat curl mite andWheat streak mosaic virus in various wheat-Haynaldia villosa 6V and 6VS lines. Plant Dis. 86: 423–428.

    Google Scholar 

  • Li Z.S., Rong S., Chen S.Y., Zhong G.C. and Mu S.M. 1985. Wheat Wide Hybridization. Chinese Scientific Press, Beijing, China.

    Google Scholar 

  • Mahmood T., Hein G.L. and Jensen S.G. 1998. Mixed infection of hard red winter wheat with high plains virus and wheat streak mosaic virus from wheat curl mites in Nebraska. Plant Dis. 82: 311–315.

    Google Scholar 

  • Martin T.J., Harvey T.L., Bender C.G. and Seifers D.L. 1984. Control of wheat streak mosaic virus with vector resistance in wheat. Phytopathology 74: 963–964.

    Google Scholar 

  • Montana J.R., Jacobs J.L., Hunger R.M. and Sherwood J.L. 1994. First reports ofAgropyron mosaic virus in wheat and mixed infection with wheat streak mosaic virus in Oklahoma. Plant Dis. 78: 432

    Google Scholar 

  • Montana J.R., Hunger R.M. and Sherwood J.L. 1996. Serological characterization of wheat streak mosaic virus isolates. Plant Dis. 80: 1239–1244.

    Google Scholar 

  • Pfannenstiel M.A. and Niblett C.L. 1978. The nature of the resistance of agrotricums to wheat streak mosaic virus. Phytopathology 68: 1204–1209.

    Article  Google Scholar 

  • Ranieri R., Lister R.M. and Burnett P.A. 1993. Relationships between barley yellow dwarf virus titer and symptom expression in barley. Crop Sci. 33: 968–973.

    Article  Google Scholar 

  • Seifers D.L. and Martin T.J. 1988. Correlation of low level wheat streak mosaic virus resistance in Triumph 64 wheat with low virus titer. Phytopathology 78: 703–707.

    Google Scholar 

  • Seifers D.L., Martin T.J., Harvey T.L. and Gill B.S. 1995. Temperature sensitivity and efficacy of wheat streak mosaic virus resistance derived fromAgropyron intermedium. Plant Dis. 79: 1104–1106.

    Google Scholar 

  • Shahwan I.M. and Hill J.P. 1984. Identification and occurrence of wheat streak mosaic virus in winter wheat in Colorado and its effects on several wheat cultivars. Plant Dis. 68: 579–581.

    Google Scholar 

  • Sherwood J.L. 1987. Comparison of a filter paper immunobinding assay. Western blotting and an enzyme linked immunosorbent assay for detection of wheat streak mosaic virus. J. Phytopathol. 118: 68–75.

    CAS  Google Scholar 

  • Skaria M., Lister R.M., Foster J.E. and Shaner G. 1985. Virus content as an index of symptomatic resistance to barley yellow dwarf virus in cereals. Phytopathology 75: 212–216.

    Article  Google Scholar 

  • Steel R.G.D. and Torrie J.H. 1960. Principles and Procedures of Statistics. McGraw-Hill, New York, NY.

    Google Scholar 

  • Stoddard S.L., Gill B.S. and Lommel S.A. 1987. Genetic expression of wheat streak mosaic virus resistance in two wheat-wheatgrass hybrids. Crop Sci. 27: 514–519.

    Article  Google Scholar 

  • Stoddard S.L., Lommel S.A. and Gill B.S. 1987. Evaluation of wheat germ plasm for resistance to wheat streak mosaic virus by symptomatology. ELISA, and slot-blot hybridization. Plant Dis. 71: 714–719.

    Google Scholar 

  • Sun S.C. 1981. The approach and methods of breeding new varieties and new species fromAgrotriticum hybrids. Acta Agron. Sin. 7: 51–58.

    Google Scholar 

  • Thomas J.B. and Conner R.L. 1986. Resistance to colonization by the wheat curl mite inAegilops squarrosa and its inheritance after transfer to common wheat. Crop Sci. 26: 527–530.

    Article  Google Scholar 

  • Wang R.R.-C. and Zhang X.Y. 1996. Characterization of translocated chromosome using fluorescencein situ hybridization and random amplified polymorphic DNA on twoTriticum aestivum-Thinopyrum intermedium translocation lines resistant to wheat streak mosaic virus or barley yellow dwarf virus. Chrom. Res. 4: 583–587.

    CAS  PubMed  Google Scholar 

  • Wiese M.V. 1987. Wheat streak mosaic. In: Compendium of Wheat Diseases 2nd edn. American Phytopathological Society Press, St. Paul, MN, pp. 80–81.

    Google Scholar 

  • Wells D.D., Sze-Chung Wong R., Lay C.L., Gardner W.S. and Buchenau G.W. 1973. Registration of CI 15092 and CI 15093 wheat germplasm (Reg. No. 34 and 35). Crop Sci. 13: 776.

    Article  Google Scholar 

Download references

Author information

Author notes

  1. Hongjie Li

    Present address: Department of Plant Pathology, Washington State University, 99164-6430, Pullman, WA, USA

Authors and Affiliations

  1. Lethbridge Research Centre, Agriculture and Agri-Food Canada, P.O. Box 3000, T1J 4B1, Lethbridge, Alberta, Canada

    Hongjie Li, Qin Chen, Robert J. Graf, André Laroche & A. D. Kuzyk

  2. Morden Research Station, Agriculture and Agri-Food Canada, Unit 100–101, Route 100, R6M 1Y5, Morden, Manitoba, Canada

    Robert L. Conner

  3. Botany Department, Brandon University, R1A 6A9, Brandon, MB, Canada

    F. Ahmad

Authors
  1. Hongjie Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert L. Conner
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qin Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert J. Graf
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. André Laroche
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. F. Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. D. Kuzyk
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

LRC Contribution No. 387-01061.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, H., Conner, R.L., Chen, Q. et al. Promising genetic resources for resistance to wheat streak mosaic virus and the wheat curl mite in wheat-Thinopyrum partial amphiploids and their derivatives. Genet Resour Crop Evol 51, 827–835 (2005). https://doi.org/10.1007/s10722-005-0001-1

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10722-005-0001-1

Key words

Search

Navigation