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Molecular characterization and expression analyses of ST8Sia II and IV in piglets during postnatal development: lack of correlation between transcription and posttranslational levels

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Abstract

The two mammalian α2,8-polysialyltransferases (polyST’s), ST8Sia II (STX) and ST8Sia IV (PST), catalyze synthesis of the α2-8-linked polysialic acid (polySia) glycans on neural cell adhesion molecules (NCAMs). The objective of this study was to clone the coding sequence of the piglet ST8Sia II and determine the mRNA expression levels of ST8Sia II, ST8Sia IV, NCAM and neuropilin-2 (NRP-2), also a carrier protein of polySia, during postnatal development. The amino acid sequence deduced from the coding sequence of ST8Sia II was compared with seven other mammalian species. Piglet ST8Sia II was highly conserved and shared 67.8 % sequence identity with ST8Sia IV. Genes coding for ST8Sia II and IV were differentially expressed and distinctly different in neural and non-neural tissues at postnatal days 3 and 38. Unexpectedly, the cellular levels of mRNA coding for ST8Sia II and IV showed no correlation with the posttranslational level of polySia glycans in different tissues. In contrast, mRNA abundance coding for NCAM and neuropilin-2 correlated with expression of ST8Sia II and IV. These findings show that the cellular abundance of ST8Sia II and IV in postnatal piglets is regulated at the level of translation/posttranslation, and not at the level of transcription, a finding that has not been previously reported. These studies further highlight differences in the molecular mechanisms controlling polysialylation in adult rodents and neonatal piglets.

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Acknowledgments

We gratefully acknowledge the financial support of Prof. Zuguo Liu, Dean, Xiamen University School of Medicine.

Funding

This study was funded by research grants from the Xiamen University School of Medicine, Xiamen City, China. Partial support was also received from a Mizutani Glycoscience Foundation Research Grant to FAT II (#130097).

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The authors declare that they have no conflict of interests.

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    Authors and Affiliations

    1. School of Medicine, Xiamen University, Xiamen City, 361005, China

      Xi Zhu, Yue Chen, Nai Zhang, Zhiqiang Zheng, Fengjun Zhao, Ni Liu, Chunlong Lv, Frederic A. Troy II & Bing Wang

    2. Department of Biochemistry and Molecular Medicine, University of California School of Medicine, Davis, CA, 95616, USA

      Frederic A. Troy II

    3. School of Animal & Veterinary Science, Charles Sturt University, Wagga Wagga, NSW, 2678, Australia

      Bing Wang

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    Frederic A. Troy II and Bing Wang contributed equally to this work.

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    Zhu, X., Chen, Y., Zhang, N. et al. Molecular characterization and expression analyses of ST8Sia II and IV in piglets during postnatal development: lack of correlation between transcription and posttranslational levels. Glycoconj J 32, 715–728 (2015). https://doi.org/10.1007/s10719-015-9622-6

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