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The karyotypes of five species of the Scinax perpusillus group (Amphibia, Anura, Hylidae) of southeastern Brazil show high levels of chromosomal stabilization in this taxon

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Abstract

Based on morphological, bioacoustics, and morphological traits, the genus Scinax has been subdivided into two major clades: S. catharinae and S. ruber. The first clade includes S. catharinae and S. perpusillus groups, whereas the second clade includes S. rostratus and S. uruguayus groups. Chromosome morphology, NOR and C-banding patterns of variation support these clades. This study aims the cytogenetic characterization of five species currently included in the S. perpusillus group: Scinax sp. (gr. perpusillus), S. arduous, S. belloni, S. cosenzai, and S. v-signatus, including standard cytogenetic techniques and repetitive DNA FISH probes. All species had 2n = 24 chromosomes. Nucleolar organizing regions occurred in chromosome pair 6 in all species, but differed in their locations among some species, suggesting a putative synaponomastic character for the clade. In S. belloni, the first chromosome pair was a metacentric, contrasting with the submetacentric first pair reported in all other species of the genus. Scinax sp. (gr. perpusillus) and S. v-signatus had similar karyotypic formulae, suggesting they are related species. Scinax cosenzai had a divergent C-banding pattern. Repetitive DNA probes hybridized more frequently in chromosomal subtelomeric regions in all species indicating recent cladogenesis in these species. Karyotypic evidence indicates unreported high levels of stabilization within S. perpusillus and in S. catharinae clade, resulting in a wealth of characters potentially informative for higher phylogenetic analyses.

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Acknowledgments

The authors wish to thanks the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), the Coordenação de Aperfeiçoamento de Nível Superior (CAPES) for financial support. The Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) issued the collect permits 33456-1 and 26157-7 to JVAL and RNF. Financial support was given to RNF through a CNPq scientific productivity fellowship. Comments of anonymous reviewers helped to improve an earlier version of this manuscript.

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Authors and Affiliations

  1. Museu de Zoologia João Moojen, Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, MG, CEP 36570-000, Brazil

    Marco Antônio Amorim Peixoto, João Victor Andrade Lacerda, Carolina Coelho-Augusto & Renato Neves Feio

  2. Laboratório de Sistemática Molecular – Beagle, Departamento de Biologia Animal, Universidade Federal de Viçosa, Viçosa, MG, CEP 36570-000, Brazil

    Marco Antônio Amorim Peixoto, Carolina Coelho-Augusto & Jorge Abdala Dergam

  3. Laboratório de Herpetologia, Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Antônio Carlos, 6627 Pampulha, Belo Horizonte, Minas Gerais, 31270-901, Brazil

    João Victor Andrade Lacerda

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  1. Marco Antônio Amorim Peixoto
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  2. João Victor Andrade Lacerda
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Correspondence to Marco Antônio Amorim Peixoto.

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Peixoto, M.A.A., Lacerda, J.V.A., Coelho-Augusto, C. et al. The karyotypes of five species of the Scinax perpusillus group (Amphibia, Anura, Hylidae) of southeastern Brazil show high levels of chromosomal stabilization in this taxon. Genetica 143, 729–739 (2015). https://doi.org/10.1007/s10709-015-9870-1

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