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Characterization of a low-density lipoprotein receptor, Lrp13, in Chinese tongue sole (Cynoglossus semilaevis) and medaka (Oryzias latipes)

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Abstract

As an important economic marine species cultured in China, Chinese tongue sole (Cynoglossus semilaevis) has interested us due to its sexual dimorphism and ZW/ZZ sex determination system. In a previous study, dmrt1 was identified as a dosage-dependent male-determining gene. In the present study, a female-specific expressed gene, cse0440, initially annotated as lrp1b-like, was identified from chromosome W of C. semilaevis. In view of the differences between cse0440 and lrp1b in terms of expression pattern, a phylogenetic analysis containing 85 LRP proteins was constructed and provided an evidence to re-annotate cse0440 as cseLRP13. In addition, two orthologues of cseLRP13 were separately identified from W and Z chromosomes: cseLRP13-W and cseLRP13-Z. The subsequent multiple sequence alignment and syntenic arrangements of LRP13 in C. semilaevis, Japanese medaka (Oryzias latipes), large yellow croaker (Larimichthys crocea), striped bass (Morone saxatilis), white perch (Morone americana) and Fugu rubripes (Takifugu rubripes) further supported this re-annotation. RT-PCR and in situ hybridization revealed that cselrp13 was exclusively expressed in the oocytes and follicles of ovaries. These results suggested that lrp13 may play important roles in female reproduction. In future, with the advancement of micromanipulation in flatfish, the detailed function of two lrp13 orthologues in C. semilaevis will be elucidated.

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Acknowledgements

This work was supported by grants from the Central Public-interest Scientific Institution Basal Research Fund CAFS (NO. 2016GH03), Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS (NO. 20603022016004), the National Natural Science Foundation of China (31530078, 31472273) and the Taishan Scholar Project of Shandong Province.

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Authors and Affiliations

  1. Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Na Wang, Ruoqing Wang, Wenteng Xu, Ying Zhu & Songlin Chen

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266235, China

    Na Wang, Wenteng Xu & Songlin Chen

  3. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Ruoqing Wang

  4. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, China

    Qiaomu Hu

  5. Marine Biology Institute of Shandong Province, Qingdao, 266104, China

    Fang Yan

Authors
  1. Na Wang
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  2. Ruoqing Wang
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  3. Qiaomu Hu
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  4. Wenteng Xu
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  5. Ying Zhu
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  6. Fang Yan
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  7. Songlin Chen
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Contributions

NW and SLC conceived and designed the experiments, while NW and QMH verified the sequences of cseLRP13 and olaLRP13 by PCR experiments. RQW conducted RT-PCR. YZ and FY participated in the experiment of in situ hybridization. NW and RQW analysed the data and wrote the paper. WTX provided valuable suggestions regarding paper organization and language fluency. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Na Wang or Songlin Chen.

Ethics declarations

The collection and handling of the animals used in this study were approved by the Animal Care and Use Committee at the Chinese Academy of Fishery Sciences, and all the experimental procedures were performed in accordance with the guidelines for the Care and Use of Laboratory Animals at the Chinese Academy of Fishery Sciences.

Competing interest

The authors declare that they have no competing interests.

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Wang, N., Wang, R., Hu, Q. et al. Characterization of a low-density lipoprotein receptor, Lrp13, in Chinese tongue sole (Cynoglossus semilaevis) and medaka (Oryzias latipes). Fish Physiol Biochem 43, 1289–1298 (2017). https://doi.org/10.1007/s10695-017-0372-1

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