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iTRAQ proteomic analysis of salinity acclimation proteins in the gill of tropical marbled eel (Anguilla marmorata)

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Abstract

Osmoregulation plays an important role in the migration process of catadromous fish. The osmoregulatory mechanisms of tropical marbled eel (Anguilla marmorata), a typical catadromous fish, did not gain sufficient attention, especially at the molecular level. In order to enrich the protein database of A. marmorata, a proteomic analysis has been carried out by iTRAQ technique. Among 1937 identified proteins in gill of marbled eel, the expression of 1560 proteins (80 %) was quantified. Compared with the protein expression level in the gill of marbled eel in freshwater (salinity of 0 ‰), 336 proteins were up-regulated and 67 proteins were down-regulated in seawater (salinity of 25 ‰); 33 proteins were up-regulated and 32 proteins were down-regulated in brackish water (salinity of 10 ‰). These up-regulated proteins including Na+/K+-ATPase, V-type proton ATPase, sodium–potassium–chloride co-transporter and heat shock protein 90 were enriched in many KEGG-annotated pathways, which are related to different functions of the gill. The up-regulated oxidative phosphorylation and seleno-compound metabolism pathways involve the synthesis and consumption of ATP, which represents extra energy consumption. Another identified pathway is the ribosome pathway in which a large number of up-regulated proteins are involved. It is also more notable that tight junction and cardiac muscle contraction pathways may have correlation with ion transport in gill cells. This is the first report describing the proteome of A. marmorata for acclimating to the change of salinity. These results provide a functional database for migratory fish and point out some possible new interactions on osmoregulation in A. marmorata.

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Acknowledgments

This study was supported by Natural Science Foundation of Jiangsu Province in China (BK20141450), National Natural Science Foundation of China (30770283), Project Foundation of the Academic Program Development of Jiangsu Higher Education Institution (PAPD) and the Innovation Project of Graduate Student Training of Jiangsu Province (CXLX13-381).

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Authors and Affiliations

  1. Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, 210023, China

    Yihe Jia, Shaowu Yin, Li Li, Peng Li, Fenfei Liang, Xiaolu Wang, Xiaojun Wang & Li Wang

  2. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, 222005, China

    Yihe Jia, Shaowu Yin, Li Li, Peng Li, Fenfei Liang, Xiaolu Wang, Xiaojun Wang & Li Wang

  3. Department of Life Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, UK

    Xinhua Su

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  1. Yihe Jia
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  2. Shaowu Yin
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  3. Li Li
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  4. Peng Li
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  5. Fenfei Liang
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  6. Xiaolu Wang
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  8. Li Wang
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Correspondence to Shaowu Yin.

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Jia, Y., Yin, S., Li, L. et al. iTRAQ proteomic analysis of salinity acclimation proteins in the gill of tropical marbled eel (Anguilla marmorata). Fish Physiol Biochem 42, 935–946 (2016). https://doi.org/10.1007/s10695-015-0186-y

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