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Biochemical Composition of Seminal Plasma and Annual Variations in Semen Characteristics of Jundiá Rhamdia quelen (Quoy and Gaimard, Pimelodidae)

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Abstract

This study investigated the composition of milt of the South American silver catfish (Rhamdia quelen) or jundiá. The semen was taken from jundiá in different periods during the four seasons. The biochemical composition of seminal fluid and the characteristics of sperm were analyzed. The semen quantity which can be extracted per fish in one day was 0.95 ± 0.08 ml during spring (maximum) and 0.24 ± 0.03 ml during winter (minimum). Sperm density (spermatocrit) showed higher values in the spring (75.1 ± 1.3%) decreasing slightly afterwards and reaching 63.0 ± 2.4% to 65.0 ± 2.2% in the fall and winter. Immediately after water dilution, 90–100% of the spermatozoa presented vigorous straightforward motility that remained for at least 20 s. The total duration of the motility was 47.9 ± 1.3 s in the spring and 38.6 ± 0.6 s in the other seasons (P < 0.05). This pattern of motility is maintained for more than 2 h after storage of the milt at room temperature. The pH from 5 to 10 of the water dilution does not influence the sperm motility. The mean seminal pH and osmolality values were 8.7 ± 0.07 and 274.8 ± 11.2 (mOsm/kg), respectively. The ion concentration was: Na 153.7 ± 2.4, K 10.7 ± 2.4, Cl 139.4 ± 2.1, Ca 4.2 ± 0.2, Mg 0.9 ± 0.05, P 0.9 ± 0.08 (mEq/l). The total protein was 0.6 ± 0.05 mg/dl and cholesterol concentration was 13.9 ± 0.9 mg/dl.

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Authors and Affiliations

  1. Laboratório de Endocrinologia Experimental, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, UFRGS, Sarmento Leite 500, CEP 90050 170, Porto Alegre, RS, Brazil

    Adriana Borges, Daniele Rodrigues Siqueira, Daiane Follmann Jurinitz, Rebeca Zanini, Fernanda do Amaral, Marcelo Lacerda Grillo & Guillermo Federico Wassermann

  2. Departmento de Patologia Clínica Veterinária, Universidade Federal do Rio Grande do Sul, UFRGS, Sarmento Leite 500, CEP 90050 170, Porto Alegre, RS, Brazil

    Eneder Rosana Oberst

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  1. Adriana Borges
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  2. Daniele Rodrigues Siqueira
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Correspondence to Adriana Borges.

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Borges, A., Siqueira, D.R., Jurinitz, D.F. et al. Biochemical Composition of Seminal Plasma and Annual Variations in Semen Characteristics of Jundiá Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem 31, 45–53 (2005). https://doi.org/10.1007/s10695-005-4742-8

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