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Identification of genetic sources with attenuated Tomato chlorosis virus-induced symptoms in Solanum (section Lycopersicon) germplasm

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Abstract

The whitefly-transmitted Tomato chlorosis virus (ToCV) (genus Crinivirus) is associated with yield and quality losses in field and greenhouse-grown tomatoes (Solanum lycopersicum) in South America. Therefore, the search for sources of ToCV resistance/tolerance is a major breeding priority for this region. A germplasm of 33 Solanum (Lycopersicon) accessions (comprising cultivated and wild species) was evaluated for ToCV reaction in multi-year assays conducted under natural and experimental whitefly vector exposure in Uruguay and Brazil. Reaction to ToCV was assessed employing a symptom severity scale and systemic virus infection was evaluated via RT-PCR and/or molecular hybridization assays. A subgroup of accessions was also evaluated for whitefly reaction in two free-choice bioassays carried out in Uruguay (with Trialeurodes vaporariorum) and Brazil (with Bemisia tabaci Middle-East-Asia-Minor1—MEAM1 = biotype B). The most stable sources of ToCV tolerance were identified in Solanum habrochaites PI 127827 (mild symptoms and low viral titers) and S. lycopersicum ‘LT05’ (mild symptoms but with high viral titers). These two accessions were efficiently colonized by both whitefly species, thus excluding the potential involvement of vector-resistance mechanisms. Other promising breeding sources were Solanum peruvianum (sensu lato) ‘CGO 6711’ (mild symptoms and low virus titers), Solanum chilense LA1967 (mild symptoms, but with high levels of B. tabaci MEAM1 oviposition) and Solanum pennellii LA0716 (intermediate symptoms and low level of B. tabaci MEAM1 oviposition). Additional studies are necessary to elucidate the genetic basis of the tolerance/resistance identified in this set of Solanum (Lycopersicon) accessions.

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Acknowledgements

This work was done in the context of MG-A doctoral studies program, Facultad de Agronomía, Universidad de la República Oriental del Uruguay. We thank A. Manzzioni, M. Giambiasi, A. Britos and L. Rubio of INIA Salto Grande, and W. Pereira and A. Costa of Embrapa Hortaliças, for their assistance in conducting some of the experiments. LSB and MENF were supported by CNPq grants. EM was supported by Grant AGL2016-75819-C2-2 and RF-M was supported by Grant AGL2015-65246-R, funded by the Ministerio de Economía y Competitividad, Spain, with assistance from the European Regional Development Fund (ERDF) and the European Social Fund (ESF).

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Authors and Affiliations

  1. Instsituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA Salto Grande, CP 50000, Salto, Uruguay

    Matías González-Arcos & Ana Arruabarrena

  2. Nacional Center for Vegetable Crops Research (CNPH), Embrapa Hortaliças, Brasília, DF, Brazil

    Maria Esther de Noronha Fonseca, Mirtes F. Lima, Miguel Michereff-Filho & Leonardo S. Boiteux

  3. Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Universidad de Málaga, E-29750, Algarrobo-Costa, Málaga, Spain

    Enrique Moriones & Rafael Fernández-Muñoz

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  1. Matías González-Arcos
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  2. Maria Esther de Noronha Fonseca
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  3. Ana Arruabarrena
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  4. Mirtes F. Lima
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  5. Miguel Michereff-Filho
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  6. Enrique Moriones
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  7. Rafael Fernández-Muñoz
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  8. Leonardo S. Boiteux
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Correspondence to Matías González-Arcos.

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González-Arcos, M., de Noronha Fonseca, M.E., Arruabarrena, A. et al. Identification of genetic sources with attenuated Tomato chlorosis virus-induced symptoms in Solanum (section Lycopersicon) germplasm. Euphytica 214, 178 (2018). https://doi.org/10.1007/s10681-018-2253-9

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