Skip to main content

Advertisement

SpringerLink
Log in

Genetic enhancement of Dubraj, a specialty rice through pedigree approach employing marker based selection for plant stature, aroma and grain quality

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Grain quality is an important feature of the marketing strategies of the rice industry in both domestic and international markets as quality influences the market price. With the demand for aromatic rice increasing steadily, export of short grain aromatic rices having premium grain quality is a viable option as the cultivation of basmati rices is limited to a specific geographic region. However, these native premium native aromatic rices require significant improvements in yield, plant stature and response to fertilizer and as grain quality traits are complex, their retention in the recombinants is a major challenge. With the recent advances in molecular markers, selection of recombinants with desirable traits is feasible through precise tracking of the target traits. The present study reports the successful recovery of the premium grain quality traits of Dubraj in IET 21044 (L9), a derivative from Pusa 44/Dubraj cross combination using pedigree approach employing markers associated with plant stature, aroma and grain quality and stringent phenotypic selection. The validation of the superior yield potential and premium grain quality of IET 21044 in the multi location evaluation trials over a 4 year period resulted in its release thus making it the first semi dwarf, high yielding, short grain aromatic rice released in India at the national level. The study was a successful demonstration of the limitless potential of the marker technology in breeding specialty rices.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Anonymous (2004) Laboratory manual on rice grain quality procedure. Directorate of Rice Research, Rajendranagar, Hyderabad, India, pp 1–20

  • Asano K, Takashi T, Miura K, Qian Q, Kitano H, Matsuoka M, Ashikari M (2007) Genetic and molecular analysis of utility of sd1 alleles in rice breeding. Breed Sci 57:53–58

    Article  CAS  Google Scholar 

  • Ashikari M, Sasaki A, Ueguchi-Tanaka M, Itoh H, Nishimura A, Datta S, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Atsuoka M (2002) Loss-of-function of a rice gibberellin biosynthetic gene, GA20 oxidase (GA20ox-2), led to the rice green evolution. Breed Sci 52:143–150

    Article  CAS  Google Scholar 

  • Bhatia D, Rajiv S, Yogesh V, Mangat GS, Ritu M, Neerja S, Jagjeet S, Naveen S, Bharaj TS, Singh K (2011) Marker-assisted development of bacterial blight resistant, dwarf, and high yielding versions of two traditional basmati rice cultivars. Crop Sci 51:759–770

    Article  Google Scholar 

  • Bradbury LM, Fitzgerald TL, Henry RJ, Jin Q, Waters DL (2005) The gene for fragrance in rice. Plant Biotechnol J 3:363–370

    Article  CAS  PubMed  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Directorate of Rice Research (2009). Progress report (2008) varietal improvement, vol 1. AICRIP (ICAR) Directorate of Rice Research, Hyderabad, pp 1.384–1.388

  • Directorate of Rice Research (2010) Progress report (2009) Varietal Improvement, vol 1. AICRIP (ICAR) Directorate of Rice Research, Hyderabad, pp 1.304–1.308

  • Directorate of Rice Research (2011) Progress report (2010) Varietal improvement, vol 1. AICRIP (ICAR) Directorate of Rice Research, Hyderabad, pp 1.319–1.326

  • Directorate of Rice Research (2012a) Progress report (2011) Varietal Improvement, vol 1. AICRIP (ICAR) Directorate of Rice Research, Hyderabad, pp 1.384–1.399

  • Directorate of Rice Research (2012b) Draft proceedings of 47th Annual Group Meetings, 2012 AICRIP (ICAR) Directorate of Rice Research, Hyderabad, p 53

  • Ellis MH, Spielmeyer W (2002) Perfect markers for the semi dwarfing gene sd1 in rice. Int Rice Res Notes 27:13–14

    Google Scholar 

  • Juliano BO (1985) Rice. Chemistry and Technology. American Association of Cereal Chemists, Saint Paul

    Google Scholar 

  • Kwon SW, Cho YC, Lee JH, Suh JP, Choi IS, Oh MK, Kim YG, Kim MK, Koh HJ, Yang SJ (2007) Linkage map construction and QTL analysis in the cross between two japonica cultivars. Korean J Breed Sci 39:94

    Google Scholar 

  • Lisa M, Noriyuki K, Rika Y, Junko S, Haruka M, Yumiko M, Masao T, Mizuho S, Shinobu N, Yuzo M (2002) Positional cloning of rice semi dwarfing gene, sd-1: rice “Green Revolution Gene” encodes a mutant enzyme involved in gibberellin synthesis. DNA Res 9:11–17

    Article  Google Scholar 

  • Liu X, Gu M, Han Y, Ji Q, Lu J, Gu S, Zhang R, Li X, Chen J, Schuyler SK, Xu M (2004) Developing gene-tagged molecular markers for functional analysis of starch-synthesizing genes in rice (Oryza sativa L.). Euphytica 135:345–353

    Article  CAS  Google Scholar 

  • Liu Q, Li Q, Cai X, Wang H, Tang S, Yu H, Wang Z, Gu M (2006) Molecular marker-assisted selection for improved cooking and eating quality of two elite parents of hybrid rice. Crop Sci 46:2354–2360

    Article  CAS  Google Scholar 

  • Murai M, Takamure I, Sato S, Tokutome T, Sato Y (2002) Effects of the dwarfing gene originating from Dee-geo-woo-gen on yield and its related traits in rice. Breed Sci 52:95–100

    Article  Google Scholar 

  • Nagaraju J, KathirvelM Ramesh KR, SiddiqEA Hasnain SE (2002) Genetic analysis of traditional and evolved basmati and non-basmati rice varieties by using fluorescence-based ISSR-PCR and SSR markers. Proc Natl Aca Sci USA 99:5836–5841

    Article  CAS  Google Scholar 

  • Patnaik A, Chaudhary D, Rao GJN (2006) Genetic improvement of long grain aromatic rices through mutation approach. Plant Mutat Rep 1:11–16

    Google Scholar 

  • Patnaik A, Chaudhary D, Rao GJN (2013) Poorna Bhog, a high yielding mutant of rice. Plant Mutat Rep 3:45–48

    Google Scholar 

  • Ramkumar G, Sivaranjani A, Pandey M, Sakthivel K, Shobha Rani N, Sudarshan I, Prasad G, Neeraja CN, Sundaram R, Viraktamath B, Madhav M (2010) Development of a PCR-based SNP marker system for effective selection of kernel length and kernel elongation in rice. Mol Breed 26:735–740

    Article  Google Scholar 

  • Rohlf FJ (1998) NTCYS-pc (Numerical taxonomy and multivariate analysis system). Version 2.0. Applied Biostatistics Inc., Port Jefferson

  • Rutger JN (1983) Application of induced and spontaneous mutation in rice breeding and genetics. Adv Agron 36:383–413

    Article  Google Scholar 

  • Sahu RK, Singh RK (2003) The aromatic rices of Madhya Pradesh and Chattisgarh. In: A treatise on the scented rices of India, 1st ed. Kalyani Publ, New Delhi, pp 327–338

  • Sarawgi AK, Parikh M, Sharma B (2012) Agro-morphological and quality characterization of Dubraj group from aromatic rice germplasm of Chhattisgarh and Madhya Pradesh. Vegetos 25:387–394

    Google Scholar 

  • Sasaki A, Ashikari M, Ueguchi-Tanaka M, Itoh H, Nishimura A, Swapan D, Ishiyama K, Saito T, Kobayashi M, Khush GS, Kitano H, Matsuoka M (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416:701–702

    Article  CAS  PubMed  Google Scholar 

  • Shobha Rani N, Krishnaiah K (2001) Current status and future prospects for improvement of aromatic rices in India. In: Duffy R, Chaudhary RC, Tram DV (eds) Specialty rices of the world: breeding, production and marketing. Science Publishers Inc, Enfield, pp 49–78

    Google Scholar 

  • Singh RK, Singh US (2003) Scented rices of India: An introduction. In: A treatise on the scented rices of India, 1st ed. 1–4

  • Sood BC, Siddiq EA (1978) A rapid technique for scent determination in rice. Ind J Genet Plant Breed 38:268–271

    Google Scholar 

  • Spielmeyer W, Ellis MH, Chandler PM (2002) Semi dwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci USA 99:9043–9048

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Thomas RH, Coffmann WR, Cabanilla VL (1980) Ancestry of improved cultivars of Asian rice. Crop Sci 20:721–727

    Article  Google Scholar 

  • Toojinda T, Tragoonrung S, Vanavichit A, Siangliw JL, Pa-In N, Jantaboon J, Siangliw M, Fukai S (2005) Molecular breeding for rainfed lowland rice in the Mekong region. Plant Prod Sci 8:330–333

    Article  CAS  Google Scholar 

  • Wada T, Uchimura Y, Ogata T, Tsubone M, Matsue Y (2006) Mapping of QTLs for physicochemical properties in japonica rice. Breed Sci 56:253–260

    Article  CAS  Google Scholar 

  • Yamazaki K, Tanabu K, Takadate M, Mikami T, Arima K, Kawamura Y, Tatsuta H, Narnioka M, Kanazawa T, Ono K, Mimoto H (1987) A new rice cultivar “Mutsuhomare”. Bull Aomori Agric Exp Stn 30:1–17

    Google Scholar 

  • Zhang Q (2007) Strategies for developing green super rice. Proc Natl Acad Sci USA 104:16402–16409

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang SL, Ni DH, Yi CX, Li L, Wang XF, Wang ZY, Yang JB (2005) Lowering grain amylose content in backcross off springs of indica rice variety 057 by molecular marker-assisted selection. Rice Sci 12:157–162

    CAS  Google Scholar 

  • Zhou PH, Tan YF, He YQ, Xu CG, Zhang Q (2003) Simultaneous improvement for four quality traits of Zhenshan 97, an elite parent of hybrid rice, by molecular marker-assisted selection. Theor Appl Genet 106:326–331

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors are highly grateful to the Director, Central Rice Research Institute, Cuttack for providing all the necessary facilities.

Author information

Authors and Affiliations

  1. Division of Crop Improvement, Central Rice Research Institute, Cuttack, 753006, Orissa, India

    A. Patnaik, P. S. Roy, G. J. N. Rao, S. S. C. Patnaik & S. G. Sharma

Authors
  1. A. Patnaik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. S. Roy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. J. N. Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. S. C. Patnaik
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. S. G. Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. J. N. Rao.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 713 kb)

Supplementary material 2 (DOC 79 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Patnaik, A., Roy, P.S., Rao, G.J.N. et al. Genetic enhancement of Dubraj, a specialty rice through pedigree approach employing marker based selection for plant stature, aroma and grain quality. Euphytica 205, 659–669 (2015). https://doi.org/10.1007/s10681-015-1382-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-015-1382-7

Keywords

Search

Navigation