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Gene expression differences associated with growth vigor in Populus full-sib allotriploid progeny following manipulated first division restitution of the diploid maternal parent

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Abstract

Polyploidy has played an important role in Populus breeding because allopolyploid trees generally display characteristics of increased height growth over diploids, and tree growth is determined by cell division and expansion in the apical region. However, the transcriptome characteristics of the shoot apical meristem involved in stem segment elongation and plant height growth remain unclear. In this study, apical buds were sampled from high and low growth vigor plants from a single full-sib cross between the parental clones, and their gene expression profiles were compared using high-throughput sequencing. In total, 4,870 genes were identified with differential expression between the high growth vigor allotriploid and its parents, and 821 genes were nonadditively expressed in the high growth vigor allotriploid. The differences in gene expression between the allotriploid and the male parent were more significant than those between it and its female parent. Many of the differentially expressed genes related to cell proliferation, meristem initiation, transcription factors, DNA and RNA methylation. Our results indicated that Populus allotriploids can generate extensive transcriptomic diversity compared with their parents. In addition, we found that the DEGs between high and low growth vigor Populus allotriploids are involved in important biological processes. Major changes in gene expression probably provide a driving force for phenotypic variation and adaptation in allopolyploids. The DEGs selected in this study provide important insight to obtain a better understanding of height growth heterosis in Populus allotriploids.

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Acknowledgments

This work was supported in part by Special Fund for Forest Scientific Research in the Public Welfare (201404113), a Foundation for the Author of National Excellent Doctoral Dissertation of P. R. China (Grant No. 201267), and 111 Project (B13007). The authors would like to thank Dr. Guanjing Hu in Department of Ecology, Evolution, and Organismal Biology, Iowa State University, Ames IA, for her critical reading of the manuscript. The authors also wish to thank Drs. Dai Chen and Bo Zhang (Novel Bioinformatics Ltd., Co, Shanghai, China) for their technical assistance in bioinformatics analysis.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, People’s Republic of China

    Shiping Cheng, Zhen Huang, Yujing Suo, Jun Wang & Xiangyang Kang

  2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, People’s Republic of China

    Shiping Cheng, Zhen Huang, Yujing Suo, Jun Wang & Xiangyang Kang

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  1. Shiping Cheng
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Correspondence to Xiangyang Kang.

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Cheng, S., Huang, Z., Suo, Y. et al. Gene expression differences associated with growth vigor in Populus full-sib allotriploid progeny following manipulated first division restitution of the diploid maternal parent. Euphytica 203, 683–700 (2015). https://doi.org/10.1007/s10681-014-1316-9

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