Abstract
Heat stress adversely affects wheat production in many regions of the world and is particularly detrimental during reproductive development. The objective of this study was to identify novel quantitative trait loci (QTL) associated with improved heat tolerance in wheat (Triticum aestivum L.) and to confirm previous QTL results. To accomplish this, a recombinant inbred line (RIL) population was subjected to a three-day 38°C daytime heat stress treatment during early grain-filling. At maturity, a heat susceptibility index (HSI) was calculated from the reduction of three main spike yield components; kernel number, total kernel weight, and single kernel weight. The HSI, as well as temperature depression (TD) of the main spike and main flag leaf during heat stress were used as phenotypic measures of heat tolerance. QTL analysis identified 14 QTL for HSI, with individual QTL explaining from 4.5 to 19.3% of the phenotypic variance. Seven of these QTL co-localized for both TD and HSI. At all seven loci, the allele for a cooler flag leaf or spike temperature (up to 0.81°C) was associated with greater heat tolerance, indicated by a lower HSI. In a comparison to previous QTL results in a RIL population utilizing the same source of heat tolerance, seven genome regions for heat tolerance were consistently detected across populations. The genetic effect of combining three of these QTL, located on chromosomes 1B, 5A, and 6D, demonstrate the potential benefit of selecting for multiple heat tolerance alleles simultaneously. The genome regions identified in this study serve as potential target regions for fine-mapping and development of molecular markers for more rapid development of heat tolerant germplasm.
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References
Assad MT, Paulsen GM (2002) Genetic changes in resistance to environmental stresses by U.S. Great Plains wheat cultivars. Euphytica 128:87–96
Ayeneh A, van Ginkel M, Reynolds MP, Ammar K (2002) Comparison of leaf, spike, peduncle and canopy temperature depression in wheat under heat stress. Field Crops Res 79:173–184
Balota M, William AP, Evett SR, Peters TR (2008) Morphological and physiological traits associated with canopy temperature depression in three closely related wheat lines. Crop Sci 48:1897–1910
Cuthbert JL, Somers DJ, Brule-Babel AL, Brown PD, Crow GH (2008) Molecular mapping of quantitative trait loci for yield and yield components in spring wheat (Triticum aestivum L.). Theor Appl Genet 117:595–608
Dellaporta SL, Wood J, Hicks JB (1983) Maize DNA minipreps. Maize Genet Coop News Lett 57:26–29
Fischer RA, Maurer R (1978) Drought resistance in spring wheat cultivars: 1. grain-yield responses. Aust J Agric Res 29:897–912
Frova C, Sarigorla M (1994) Quantitative trait loci (QTLs) for pollen thermotolerance detected in maize. Mol Gen Genet 245:424–430
Groos C, Robert N, Bervas E, Charmet G (2003) Genetic analysis of grain protein-content, grain yield and thousand-kernel weight in bread wheat. Theor Appl Genet 106:1032–1040
Hays DB, Do JH, Mason RE, Morgan G, Finlayson SA (2007a) Heat stress induced ethylene production in developing wheat grains induces kernel abortion and increased maturation in a susceptible cultivar. Plant Sci 172:1113–1123
Hays DB, Mason RE, Do JH, Menz M, Reynolds M (2007b) Expression quantitative trait loci mapping heat tolerance during reproductive development in wheat (Triticum aestivum). In: Buck HT, Nisi JE, Salomón N (eds) Wheat production in stressed environments. Springer, pp 373–383
Hede AR, Skovmand B, Reynolds MP, Crossa J, Vilhelmsen AL, Stolen O (1999) Evaluating genetic diversity for heat tolerance traits in Mexican wheat landraces. Genet Resour Crop Evol 46:37–45
Kuchel H, Williams K, Langridge P, Eagles H, Jefferies S (2007a) Genetic dissection of grain yield in bread wheat. I. QTL analysis. Theor Appl Genet 115:1029–1041. doi:10.1007/s00122-007-0629-7
Kuchel H, Williams K, Langridge P, Eagles H, Jefferies S (2007b) Genetic dissection of grain yield in bread wheat. II. QTL-by-environment interaction. Theor Appl Genet 115:1015–1027. doi:10.1007/s00122-007-0628-8
Mason RE, Mondal S, Beecher FW, Pacheco A, Jampala B, Ibrahim AMH, Hays DB (2010) QTL associated with heat susceptibility index in wheat (Triticum aestivum L.) under short-term reproductive stage heat stress. Euphytica 174:423–436
Mathews KL, Malosetti M, Chapman S, McIntyre L, Reynolds M, Shorter R, Fv Eeuwijk (2008) Multi-environment QTL mixed models for drought stress adaptation in wheat. Theor Appl Genet 117:1077–1091
Pinto S, Chapman SC, McIntyre CL, Shorter R, Reynolds MP (2008) QTL for canopy temperature response related to yield in both heat and drought environments. In: Proceedings of the 11th international wheat genetics symposium, Brisbane, Australia
Rebetzke GJ, Ellis MH, Bonnett DG, Richards RA (2007) Molecular mapping of genes for coleoptile growth in bread wheat (Triticum aestivum L.). Theor Appl Genet 114:1173–1183
Reynolds MP, Saint Pierre C, Saad ASI, Vargas M, Condon AG (2007) Evaluating potential genetic gains in wheat associated with stress-adaptive trait expression in elite genetic resources under drought and heat stress. Crop Sci 47:S172–S189
Richards RA (2000) Selectable traits to increase crop photosynthesis and yield of grain crops. J Exp Bot 51:447–458
Ristic Z, Momcilovic I, Bukovnik U, Prasad PVV, Fu JM, DeRidder BP, Elthon TE, Mladenov N (2009) Rubisco activase and wheat productivity under heat-stress conditions. J Exp Bot 60:4003–4014
Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Savin R, Stone PJ, Nicolas ME, Wardlaw IF (1997) Grain growth and malting quality of barley 1: effects of heat stress and moderately high temperature. Aust J Agric Res 48:615–624
Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114
Wang D, Shi J, Carlson SR, Cregan PB, Ward RW, Diers BW (2003) A low-cost, high-throughput polyacrylamide gel electrophoresis system for genotyping with microsatellite DNA markers. Crop Sci 43:1828–1832
Wang S, Basten CJ, Zeng ZB (2007) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC
Yang J, Sears RG, Gill BS, Paulsen GM (2002a) Genotypic differences in utilization of assimilate sources during maturation of wheat under chronic heat and heat shock stresses—utilization of assimilate sources by wheat under heat stresses. Euphytica 125:179–188
Yang J, Sears RG, Gill BS, Paulsen GM (2002b) Growth and senescence characteristics associated with tolerance of wheat-alien amphiploids to high temperature under controlled conditions. Euphytica 126:185–193
Yang J, Sears RG, Gill BS, Paulsen GM (2002c) Quantitative and molecular characterization of heat tolerance in hexaploid wheat. Euphytica 126:275–282
Acknowledgment
This project is supported by the Agriculture and Food Research Initiative competitive grant no. 2010-65114-20389 from the USDA National Institute of Food and Agriculture.
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Esten Mason, R., Mondal, S., Beecher, F.W. et al. Genetic loci linking improved heat tolerance in wheat (Triticum aestivum L.) to lower leaf and spike temperatures under controlled conditions. Euphytica 180, 181–194 (2011). https://doi.org/10.1007/s10681-011-0349-6
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DOI: https://doi.org/10.1007/s10681-011-0349-6