Abstract
A novel gene, designated Pg (purple glume), controlling anthocyanin pigmentation of the glume was identified and mapped in an F2 population from the durum wheat (Triticum durum) cross TRI 15744/TRI 2719. This gene was close to one of the two complementary dominant genes, controlling anthocyanin pigmentation of the pericarp (gene Pp3) in the centromere region of chromosome 2A; the other Pp gene (Pp1) was mapped on the short arm of chromosome 7B, near gene Pc controlling anthocyanin pigmentation of the culm and co-segregating with Pls (purple leaf sheath) and Plb (purple leaf blade). On the basis of the mapping results, the Pp3, Pc, Pls and Plb genes of T. durum were regarded as allelic to the T. aestivum Pp3, Pc-B1, Pls-B1 and Plb-B1 loci. The likely allelism of Pp1 in T. durum and T. aestivum remains in dispute, the present durum Pp gene mapped to the short arm of chromosome 7B, whereas in common wheat it was reportedly located on the long arm.
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References
Ahmed N, Maekawa M, Utsugi S, Rikiishi K et al (2006) The wheat Rc gene for red coleoptile colour codes for a transcriptional activator of late anthocyanin biosynthesis genes. J Cereal Sci 44:54–58
Blanco A, Bellomo MP, Cenci A, De Giovanni C et al (1998) A genetic linkage map of durum wheat. Theor Appl Genet 97:721–728
Börner A, Schäfer M, Schmidt A, Grau M, Vorwald J (2005) Associations between geographical origin and morphological characters in bread wheat. Plant Genet Res 3:360–372
Bruce W, Folkerts O, Garnaat C, Crasta O et al (2000) Expression profiling of the maize flavonoid pathway genes controlled by estradiol-inducible transcription factors CRC and P. Plant Cell 12:65–80
De Vries JN, Sybenga J (1984) Chromosomal location of 17 monogenically inherited morphological markers in rye (Secale cereale L.) using a translocation tester set. Z Pflanzenzücht 92:117–139
Dobrovolskaya OB, Arbuzova VS, Lohwasser U, Röder MS, Börner A (2006) Microsatellite mapping of complementary genes for purple grain colour in bread wheat (Triticum aestivum L.). Euphytica 150:355–364
Flaksberger KA (1935) Wheat. In: Wulff EV (ed) Flora of cultivated plants. State Agricultural Publication Company, Moscow-Leningrad, pp 1–434 (in Russian)
Flintham JE (2000) Different genetic components control coat-imposed and embryo-imposed dormancy in wheat. Seed Sci Res 10:43–50
Ganal M, Röder MS (2007) Microsatellite and SNP markers in wheat breeding. In: Varshney RK, Tuberosa R (eds) Genomics-assisted crop improvement. Genomics applications in crops, vol 2. Springer, The Netherlands, pp 1–24
Gould KS (2004) Nature’s swiss army knife: the diverse protective roles of anthocyanins in leaves. J Biomed Biotech 5:314–320
Gulyaeva ZB (1984) Localization of the genes for pubescence of the glumes and coloration of the auricles in the leaf sheath in winter wheat variety Ul’yanovka. Bull Appl Bot Genet Plant Breed 85:95–96 (in Russian)
Himi E, Noda K (2005) Red grain colour gene (R) of wheat is a Myb-type transcription factor. Euphytica 143:239–242
Himi E, Nisar A, Noda K (2005) Colour genes (R and Rc) for grain and coleoptile upregulate flavonoid biosynthesis genes in wheat. Genome 48:747–754
Khlestkina EK, Pestsova EG, Röder MS, Börner A (2002a) Molecular mapping, phenotypic expression and geographical distribution of genes determining anthocyanin pigmentation of coleoptiles in wheat (Triticum aestivum L.). Theor Appl Genet 104:632–637
Khlestkina EK, Pestsova EG, Salina EA, Röder MS et al (2002b) Molecular mapping and tagging of wheat genes using RAPD, STS and SSR markers. Cell Mol Biol Letters 7:795–802
Khlestkina EK, Pshenichnikova TA, Röder MS, Arbuzova VS et al (2006) Comparative mapping of genes for glume colouration and pubescence in hexaploid wheat (Triticum aestivum L.). Theor Appl Genet 113:801–807
Khlestkina EK, Röder MS, Pshenichnikova TA, Simonov AV (2008a) Genes for anthocyanin pigmentation in wheat: review and microsatellite-based mapping. In: Verrity JF, Abbington LE et al (eds) Chromosome mapping research developments. NOVA Science, USA, pp 155–175
Khlestkina EK, Röder MS, Salina EA (2008b) Relationship between homoeologous regulatory and structural genes in allopolyploid genome—a case study in bread wheat. BMC Plant Biol 8:88
Khlestkina EK, Pshenichnikova TA, Röder MS, Börner A (2009a) Clustering anthocyanin pigmentation genes in wheat group 7 chromosomes. Cereal Res Comm 37:391–398
Khlestkina EK, Salina EA, Pshenichnikova TA, Röder MS, Börner A (2009b) Glume coloration in wheat: allelism test, consensus mapping and its association with specific microsatellite alleles. Cereal Res Comm 37:37–43
Lander ES, Green P, Abrahamson J, Barlow A et al (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Lundqvist U, Franckowiak JD, Konishi T (1996) New and revised descriptions of barley genes. Barley Genet Newsl 26:22–43
Martynov SP, Dobrotvorskaya TV (1997) Distribution characteristics of traits ear morphology in common wheat in the former Soviet Union. Rus J Genet 33:350–357
Maystrenko OI (1992) The use of cytogenetic methods in ontogenesis study of common wheat. In: Zhakote AG (ed) Ontogenetics of higher plants. Shtiintsa, Kishinev, pp 98–114
McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J et al (2008) Catalogue of gene symbols for wheat. http://www.grs.nig.ac.jp/wheat/komugi/genes/
Melz G, Thiele V (1990) Chromosome locations of genes controlling ‘purple leaf base’ in rye and wheat. Euphytica 49:155–159
Plaschke J, Ganal MW, Röder MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theor Appl Genet 91:1001–1007
Röder MS, Korzun V, Wendehake K, Plaschke J et al (1998) A microsatellite map of wheat. Genetics 149:2007–2023
Salina EA, Leonova IN, Efremova TT, Röder MS (2006) Wheat genome structure: translocations during the course of polyploidization. Funct Integr Genomics 6:71–80
Stein N, Prasad M, Scholz U, Thiel T et al (2007) A 1, 000-loci transcript map of the barley genome: new anchoring points for integrative grass genomics. Theor Appl Genet 114:823–839
Wang C, Shu Q (2007) Fine mapping and candidate gene analysis of purple pericarp gene Pb in rice (Oryza sativa L.). Chinese Sci Bull 52:3097–3104
Winkel-Shirley B (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol 5:218–223
Acknowledgments
We thank Renate Voss and Annette Marlow for their excellent technical assistance. The study was supported by the Russian Foundation for Basic Research (project no 08-04-00368-a), the Russian Science Support Foundation and the ‘Deutsche Forschungsgemeinschaft’ (project no BO 1423/9-1/551902).
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Khlestkina, E.K., Röder, M.S. & Börner, A. Mapping genes controlling anthocyanin pigmentation on the glume and pericarp in tetraploid wheat (Triticum durum L.). Euphytica 171, 65–69 (2010). https://doi.org/10.1007/s10681-009-9994-4
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DOI: https://doi.org/10.1007/s10681-009-9994-4