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Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents

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Abstract

To prevent lodging and sprouting damage in rye, a dominant dwarfing gene, Ddw1, has been incorporated into breeding lines to reduce plant height in Finland. However, the inability to identify heterozygous plants makes it difficult to purify breeding lines. Doubled haploidy (EM-1 × Voima) and bulked segregant analysis were used to search for an efficient PCR-based tool to identify homozygous short plants. In addition, SNP (single nucleotide polymorphism) markers were created from the endosperm-specific β-amylase gene and the microsatellite locus REMS1218 expressed in rye and known to be located near Ddw1. The best marker was a combination of the microsatellite REMS1218 and the SNP created from it, located 13 cM from the QTL corresponding to Ddw1. However, for a rye breeder, the SNP alone is an adequate tool to identify plants homozygous for the EM-1 allele and it can be used in selection for the desirable growth habit in rye.

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Acknowledgments

We thank Marja-Riitta Arajärvi, Leena Ramstedt, and Sirpa Moisander for their efficient technical assistance in DNA marker analyses. Anneli Virta and Johanna Rusi are warmly thanked for running DNA markers with the MegaBACE and for DNA sequencing. We thank Maria Erkkilä for the barley β-amylase primers. Boreal Plant Breeding Ltd. kindly provided all the rye material used in this study. Professor Alan Schulman is acknowledged for the language revision of the manuscript. The Finnish Ministry of Agriculture and Forestry, Heikki and Hilma Honkanen Foundation, the Finnish Academy of Sciences (project number 112053), and Finnish Cultural Foundation are gratefully acknowledged for financially supporting this study.

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  1. Plant Genomics, Biotechnology and Food Research, MTT Agrifood Research Finland, Myllytie 1, 31600, Jokioinen, Finland

    Teija Tenhola-Roininen & Pirjo Tanhuanpää

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  1. Teija Tenhola-Roininen
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  2. Pirjo Tanhuanpää
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Correspondence to Teija Tenhola-Roininen.

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Tenhola-Roininen, T., Tanhuanpää, P. Tagging the dwarfing gene Ddw1 in a rye population derived from doubled haploid parents. Euphytica 172, 303–312 (2010). https://doi.org/10.1007/s10681-009-9982-8

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  • DOI: https://doi.org/10.1007/s10681-009-9982-8

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