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Marker-assisted identification of resistance genes to soybean mosaic virus in soybean lines

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Abstract

Soybean plants react differentially to soybean mosaic virus (SMV) strains because of interactions among different resistant genes in the soybean genome. Three independent genes resistant to SMV have been identified by inheritance studies and linkage analyses. To develop durable SMV-resistant soybean cultivars, it is necessary to determine which soybean SMV resistance genes can be readily transferred from resistant to susceptible cultivars in a breeding system. Here, we report the type and number of resistance gene(s) in four Korean elite soybean cultivars using a combination of disease reaction symptoms, inheritance studies, and molecular marker mappings. The disease reactions of Sowonkong and Keunolkong soybean varietals in response to infection with SMV strains suggested that both cultivars most likely harbor the Rsv1 gene similar to that in York. Subsequent inheritance studies confirmed that Sowonkong has the Rsv1 gene. The inheritance studies suggested that Sinpaldalkong harbored the Rsv1 gene, which was then confirmed by molecular marker mapping. The inheritance studies also suggested that Jinpumkong 2, which is the most resistant to SMV infection among the four cultivars, contained the Rsv1 and Rsv3 genes; this was confirmed by molecular marker mapping. Our approach, which combined inheritance studies and molecular linkage analyses, allowed the efficient identification of resistance gene(s) in four Korean soybean cultivars.

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References

  • Almeida MRA, Almeida LA, Kiihl RAS, Domit LA (1995) Identification of a new strain of the Soybean mosaic virus in Brazil. Agric Biol Technol 38:1095–1100

    Google Scholar 

  • Buss GR, Ma G, Chen P, Tolin SA (1997) Registration of V94–5152 soybean germplasm resistant to soybean mosaic potyvirus. Crop Sci 37:1987–1988

    Google Scholar 

  • Buss GR, Ma G, Kristipati S, Chen P, Tolin SA (1999) A new allele at the Rsv3 locus for resistance to soybean mosaic virus. In: World soybean res conf VI, Chicago, 4–7 Aug, Proceedings 490

  • Buzzell RI, Tu JC (1989) Inheritance of a soybean stem-tip necrosis reaction to soybean mosaic virus. J Hered 80:400–401

    Google Scholar 

  • Chen P, Buss GR, Roane CW, Tolin SA (1991) Allelism among genes for resistance to soybean mosaic virus in strain-differential soybean cultivars. Crop Sci 31:305–309

    Article  Google Scholar 

  • Chen P, Buss GR, Roane CW, Tolin SA (1994) Inheritance in soybean of resistant and necrotic reactions to soybean mosaic virus strains. Crop Sci 34:414–422

    Google Scholar 

  • Chen P, Buss GR, Gunduz I, Roane CW, Tolin SA (2001) Inheritance and allelism of Raiden soybean for resistance to soybean mosaic virus. J Hered 92:51–55. doi:10.1093/jhered/92.1.51

    Article  PubMed  CAS  Google Scholar 

  • Chen P, Buss GR, Tolin SA, Gunduz I, Cicek M (2002) A valuable gene in Suwon 97 soybean for resistance to soybean mosaic virus. Crop Sci 42:333–337

    Article  Google Scholar 

  • Cho EK, Goodman RM (1979) Strains of soybean mosaic virus: classification based on virulence in resistant soybean cultivars. Phytopathol 69:467–470. doi:10.1094/Phyto-69-467

    Article  Google Scholar 

  • Cho EK, Goodman RM (1982) Evaluation of resistance in soybean to soybean mosaic virus strains. Crop Sci 22:1133–1136

    Google Scholar 

  • Cho EK, Choi SH, Cho WT (1983) Newly recognized soybean mosaic virus mutants and sources of resistance in soybeans. Res Rep ORD 25:18–22

    Google Scholar 

  • Edwardson JR (1974) Host ranges of viruses in the PVY group. FL Agric Exp Stn Monograph Ser No. 5, p 225

  • Gore MA, Hayes AJ, Jeong SC, Yue YG, Buss GR, Saghai Maroof MA (2002) Mapping tightly linked genes controlling potyvirus infection at the Rsv1 and Rpv1 region in soybean. Genome 45:592–599. doi:10.1139/g02-009

    Article  PubMed  CAS  Google Scholar 

  • Gunduz I, Buss GR, Chen P, Tolin SA (2002) Characterization of SMV resistance genes in Tousan 140 and Hourei soybean. Crop Sci 42:90–95

    Article  PubMed  Google Scholar 

  • Gunduz I, Buss GR, Chen P, Tolin SA (2004) Genetic and phenotypic analysis of soybean mosaic virus resistance in PI 88788 soybean. Phytopathol 84:687–692. doi:10.1094/PHYTO.2004.94.7.687

    Article  Google Scholar 

  • Hayes AJ, Ma G, Buss GR, Saghai Maroof MA (2000) Molecular marker mapping of Rsv4, a gene conferring resistance to all known strains of soybean mosaic virus. Crop Sci 40:1434–1437

    Article  CAS  Google Scholar 

  • Hayes AJ, Jeong SC, Gore MA, Yu YG, Buss GR, Tolin SA, Saghai Maroof MA (2004) Recombination within a nucleotide-binding-site/leucine-rich-repeat gene cluster produces new variants conditioning resistance to soybean mosaic virus in soybean. Genetics 166:493–503. doi:10.1534/genetics.166.1.493

    Article  PubMed  CAS  Google Scholar 

  • Hill JH, Bailey TB, Benner HI, Tachibana H, Durand DP (1987) Soybean Mosaic Virus: effects of primary disease incidence of yield and seed quality. Plant Dis 71:237–239. doi:10.1094/PD-71-0237

    Article  Google Scholar 

  • Jeong SC, Saghai Maroof MA (2004) Detection and genotyping of SNPs tightly linked to two disease resistance loci, Rsv1 and Rsv3, of soybean. Plant Breed 123:305–310. doi:10.1111/j.1439-0523.2004.00981.x

    Article  CAS  Google Scholar 

  • Jeong SC, Kristipati S, Hayes AJ, Maughan PJ, Noffsinger SL, Gunduz I, Buss GR, Saghai Maroof MA (2002) Genetic and sequence analysis of markers tightly linked to the soybean mosaic virus resistance gene, Rsv3. Crop Sci 42:265–270

    PubMed  CAS  Google Scholar 

  • Keim P, Olson TC, Shoemaker RC (1988) A rapid protocol for isolating soybean DNA. Soybean Genet Newsl 15:150–152

    Google Scholar 

  • Kiihl RAS, Hartwing EE (1979) Inheritance of reaction to soybean mosaic virus in soybeans. Crop Sci 19:372–375

    Article  Google Scholar 

  • Kim JS, Lee EJ, Cho EK (1991) A new virulent strain of soybean mosaic virus infecting SMV resistant soybean cultivar Deogyou. Kor J Plant Pathol 7:37–41

    Google Scholar 

  • Kim SD, Hong EH, Seong YG, Kim YH, Lee SH, Kim HS, Ryu YH, Kim YS (1996) A new soybean variety for sprouting Pureunkong with green seed coat and cotyledon, good seed quality. RDA J Agric Sci 38:238–241

    Google Scholar 

  • Kim YH, Kim OS, Lee BC, Moon JK, Lee SC, Lee JY (2003) G7H, a new soybean mosaic virus strain: its virulence and nucleotide sequence of CI gene. Plant Dis 87:1372–1375. doi:10.1094/PDIS.2003.87.11.1372

    Article  CAS  Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly JM, Lincoln SE, Newberg L (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181. doi:10.1016/0888-7543(87)90010-3

    Article  PubMed  CAS  Google Scholar 

  • Liao L, Chen P, Buss GR, Yang Q, Tolin SA (2002) Inheritance and allelism of resistance to soybean mosaic virus in Zao 18 soybean from China. J Hered 93:447–452. doi:10.1093/jhered/93.6.447

    Article  PubMed  CAS  Google Scholar 

  • Ma G, Chen P, Buss GR, Tolin SA (1995) Genetic characteristics of two genes for resistance to soybean mosaic virus in PI 486355 soybean. Theor Appl Genet 91:907–914. doi:10.1007/BF00223899

    Article  CAS  Google Scholar 

  • Ma G, Chen P, Buss GR, Tolin SA (2002) Complementary action of two independent dominant genes in Columbia soybean for resistance to soybean mosaic virus. J Hered 93:179–184. doi:10.1093/jhered/93.3.179

    Article  PubMed  CAS  Google Scholar 

  • Ma G, Chen P, Buss GR, Tolin SA (2003) Genetic study of a lethal necrosis to soybean mosaic virus in PI 507389 soybean. J Hered 94:205–211. doi:10.1093/jhered/esg059

    Article  PubMed  CAS  Google Scholar 

  • Saghai Maroof MA, Jeong SC, Gunduz I, Tucker DM, Buss GR, Tolin SA (2008) Pyramiding of soybean mosaic virus resistance genes by marker-assisted selection. Crop Sci 48:517–526. doi:10.2135/cropsci2007.08.0479

    Article  CAS  Google Scholar 

  • Shi A, Chen P, Li DX, Zheng C, Hou A, Zhang B (2008) Genetic confirmation of 2 independent genes for resistance to soybean mosaic virus in J05 soybean using SSR markers. J Hered 99:598–603. doi:10.1093/jhered/esn035

    Article  PubMed  CAS  Google Scholar 

  • Silva MF, Kiihl RAS, Almeida AMR (2004) Inheritance of resistance to soybean mosaic virus in FT-10 soybean. Euphytica 135:339–343. doi:10.1023/B:EUPH.0000013371.00994.52

    Article  Google Scholar 

  • Sinclair JB (1982) Compendium of soybean diseases. The American Phytopathology Society, St. Paul, p 104

    Google Scholar 

  • Song QJ, Marek LF, Shoemaker RC, Lark KG, Concibido VC, Delannay X, Specht JE, Cregan PB (2004) A new integrated genetic linkage map of the soybean. Theor Appl Genet 109:122–128. doi:10.1007/s00122-004-1602-3

    Article  PubMed  CAS  Google Scholar 

  • Thottapilly G, Rossel HW (1987) Viruses affecting soybean. In: Singh SR, Rachie KO, Dashiell KE (eds) Soybeans for the tropics: research, production, utilization. Wiley, Hichester, pp 53–68

    Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78. doi:10.1093/jhered/93.1.77

    Article  PubMed  CAS  Google Scholar 

  • Wrather JA, Anderson TR, Arsyad DM, Tan Y, Ploper LD, Porta-Puglia A, Ram HH, Yorinori JT (2001a) Soybean disease loss estimates for the top ten soybean-producing countries in 1998. Can J Plant Pathol 23:115–121

    Google Scholar 

  • Wrather JA, Shienstra WC, Koenning SR (2001b) Soybean disease loss estimates for the United States from 1996 to 1998. Can J Plant Pathol 23:122–131

    Google Scholar 

  • Yang K, Moon J-K, Jeong N, Back K, Kim HM, Jeong SC (2008) Genome structure and segregation distortion in soybean revealed by a genome wide genetic map constructed from a single population. Genomics 92:52–59. doi:10.1016/j.ygeno.2008.03.008

    Article  PubMed  CAS  Google Scholar 

  • Yu YG, Saghai Maroof MA, Buss GR, Maughan PJ, Tolin SA (1994) RFLP and microsatellite mapping of a gene for soybean mosaic virus resistance. Phytopathol 84:60–64. doi:10.1094/Phyto-84-60

    Article  CAS  Google Scholar 

  • Zheng C, Chen P, Gergerich R (2005) Characterization of resistance to soybean mosaic virus in diverse soybean germplasm. Crop Sci 45:2503–2509. doi:10.2135/cropsci2005.0114

    Article  Google Scholar 

  • Zheng C, Chen P, Gergerich R (2006) Genetic analysis of resistance to soybean mosaic virus in J05 soybean. J Hered 97:429–437. doi:10.1093/jhered/esl024

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from the BioGreen 21 Project (code no. 20080401034011), Rural Development Administration, the Republic of Korea. Dr. K. Van is the recipient of a fellowship from the BK21 program granted by the Ministry of Education, Science and Technology, the Republic of Korea.

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Authors and Affiliations

  1. Department of Functional Crop, National Institute of Crop Science, Rural Development Administration, Milyang, 627-803, Korea

    Jung-Kyung Moon

  2. BioEvaluation Center, Korea Research Institute of Bioscience and Biotechnology, Chungbuk, 363-883, Korea

    Soon-Chun Jeong

  3. Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul, 151-921, Korea

    Kyujung Van & Suk-Ha Lee

  4. Department of Crop and Soil Environmental Sciences, Virginia Tech University, Blacksburg, VA, 20461, USA

    M. A. Saghai Maroof

  5. Plant Genomics and Breeding Institute, Seoul National University, Seoul, 151-921, Korea

    Suk-Ha Lee

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  1. Jung-Kyung Moon
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  2. Soon-Chun Jeong
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  3. Kyujung Van
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  4. M. A. Saghai Maroof
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Correspondence to Suk-Ha Lee.

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Moon, JK., Jeong, SC., Van, K. et al. Marker-assisted identification of resistance genes to soybean mosaic virus in soybean lines. Euphytica 169, 375–385 (2009). https://doi.org/10.1007/s10681-009-9970-z

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