Abstract
In this study, two laboratory-scale SBBR reactors were established in a plateau habitat. Using high flux sequencing, the SBBR process was compared by natural sediment and autotrophic sludge to characterize the functional modules and functional genes of carbon, nitrogen, and phosphorus metabolism under different working conditions and to analyze the reaction mechanism. The results showed that all the functional modules of carbon metabolism and nitrogen metabolism were found in the SBBR process, except for methane metabolism, which occurred at 25 °C in tank 2, the functional modules related to methane metabolism are enhanced at all working conditions. Except for methane metabolism, all functional genes in tank 2 are inhibited by different working conditions, whereas tank 1 shows a slight enhancement. The different working conditions in nitrogen metabolism demonstrate inhibition of functional modules and functional genes in both tanks. Oxidative phosphorylation was missing five functional modules, except for M00153, where only two genes, K00424 and K22501, are missing, all of the required genes are missing in the other four functional modules. Overall the different conditions demonstrated some inhibition in both reaction tanks of the SBBR process. It is preferable to use self-cultivated sludge for membrane acclimation when operating the SBBR process in a plateau habitat. The findings of this study can be used to further research microbial carbon, nitrogen, and phosphorus metabolism mechanisms in SBBR processes in plateau habitats.
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Funding
This work was supported by the Key Projects of Tibet Natural Fund (XZ202301ZR0056G), National Natural Science Foundation of China (NO. 51868069), 2022 National College Students Innovative Entrepreneurship Training Program General Project (SBR-MBR Craftsmanship and Microbial Community Structure Research in Plateau Habitat).
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CXy, as the main contributor to the study, designed the study experiment and analyzed the results. ZLs strictly controlled the adjustment of experimental run parameters, conducted experiments and records and organizes data. ZYc directed and completed all processes. CJq performed data samples management and participated in gene expression assay tests. HKy revised the manuscript, drew the diagrams, and wrote the revised reply.
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Chen, X., Hao, K., Zhao, L. et al. Carbon, nitrogen, and phosphorus metabolic relationships and reaction mechanisms in SBBR processes in the plateau habitat. Environ Monit Assess 195, 1464 (2023). https://doi.org/10.1007/s10661-023-11961-9
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DOI: https://doi.org/10.1007/s10661-023-11961-9