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Monitoring the effects of a lepidopteran insecticide, Flubendiamide, on the biology of a non-target dipteran insect, Drosophila melanogaster

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Abstract

Various organisms are adversely affected when subjected to chronic fluoride exposure. This highly electronegative ion present in several insecticide formulations is found to be lethal to target pests. In the present study, Drosophila melanogaster is treated with sub-lethal concentrations of a diamide insecticide formulation, Flubendiamide. Chronic exposure to the diamide (0.5–100 μg/mL) was found to be responsible for increase in fluoride ion concentration in larval as well as adult body fluid. Interestingly, 100 μg/mL Flubendiamide exposure resulted in 107 and 298% increase in fluoride ion concentration whereas only 23 and 52% of Flubendiamide concentration increase in larval and adult body fluid, respectively. Further, in this study, selected life cycle parameters like larval duration, pupal duration and emergence time showed minimal changes, whereas percentage of emergence and fecundity revealed significant treatment-associated variation. It can be noted that nearly 79% reduction in fecundity was observed with 100 μg/mL Flubendiamide exposure. The variations in these parameters indicate probable involvement of fluoride ion in detectable alterations in the biology of the non-target model insect, D. melanogaster. Furthermore, the outcomes of life cycle study suggest change in resource allocation pattern in the treated flies. The altered resource allocation might have been sufficient to resist changes in selective life cycle parameters, but it could not defend the changes in fecundity. The significant alterations indicate a definite trade-off pattern, where the treated individuals happen to compromise. Thus, survival is apparently taking an upper hand in comparison to reproductive ability in response to Flubendiamide exposure.

The figure demonstrates increase in Fluoride and Flubendiamide concentrations in Drosophila melanogaster after chronic sub-lethal exposure to Flubendiamide. Treatment-induced alterations in larval and pupal duration, reduction in fecundity and alteration in male-female ratio is also observed.

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Acknowledgements

The authors are grateful to the head, DST-FIST- and UGC DSR-sponsored Department of Zoology, The University of Burdwan, for providing the infrastructural facilities during the course of the work. We thank Dr. A Mazumdar, BU, for extending the environmental chamber facility required for the fly culture. Dr. G Aditya is also thankfully acknowledged for his kind help in statistical analysis.

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  1. Toxicological Research Unit, Cytogenetics Laboratory, Department of Zoology, The University of Burdwan, Burdwan, West Bengal, 713104, India

    Saurabh Sarkar & Sumedha Roy

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  1. Saurabh Sarkar
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Correspondence to Sumedha Roy.

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Sarkar, S., Roy, S. Monitoring the effects of a lepidopteran insecticide, Flubendiamide, on the biology of a non-target dipteran insect, Drosophila melanogaster . Environ Monit Assess 189, 557 (2017). https://doi.org/10.1007/s10661-017-6287-6

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