Abstract
In the present study, milk samples including raw and ultra-high temperature (UHT) processed milk were analyzed for pesticide residue levels, including five pesticides, viz chloripyrifos, endosulfan (α and β), profenofos and bifenthrin by gas chromatography microelectron capture detector (GC-μECD) after extraction by QuEChERS method. Further confirmation of the pesticide residue was done by GC-MS. The pesticide residual level in raw and UHT milk samples (n = 70) was determined in the range of 0.1–30 μg L−1. All UHT processed milk samples contain pesticide residues within permissible limit set by the World Health Organization (WHO); however, among raw milk samples, chloripyrifos (12 %), α (24 %), and β (14 %) endosulfan were found above the maximum residue limit (MRL). The estimated daily intake (EDI) of these four pesticide residues were also calculated as 1.32, 16.16, 5.30, 10.20, and 9.93 μg kg−1 body weight for chloripyrifos, endosulfan α, profenofos, endosulfan β, and bifenthrin, respectively. It is concluded that the raw milk samples showed higher prevalence of pesticide residues as compared to UHT processed milk.
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Arias Estevez, M., Lopez Periago, E., Martinez Carballo, E., Simal Gandara, J., Juan Carlos, M., & Garcia Rio, L. (2008). The mobility and degradation of pesticides in soils and the pollution of groundwater resources. Agriculture, Ecosystems & Environment, 123(4), 247–260. doi:10.1016/j.agee.2007.07.011.
Aslam, M., Rais, S., & Alam, M. (2013). Quantification of organochlorine pesticide residues in the buffalo milk samples of Delhi City, India. Journal of Environmental Protection, 4(9), 964–974. doi:10.4236/jep.2013.49111.
Aziz ul Hassan, Tabinda, A. B., Abbas, M., & Khan, A. M. (2014). Organochlorine and pyrethroid pesticides analysis in dairy milk samples collected from cotton growing belt of Punjab, Pakistan. Pakistan Journal of Agricultural Sciences, 51(2), 321–325.
Bajwa, U., & Sandhu, K. S. (2014). Effect of handling and processing on pesticide residues in food—a review. Journal of Food Science and Technology, 51(2), 201–220. doi:10.1007/s13197-011-0499-5.
Bedi, J. S., Gill, J. P., Aulakh, R. S., & Kaur, P. (2015). Pesticide residues in bovine milk in Punjab, India: spatial variation and risk assessment to human health. Archives of Environmental Contamination and Toxicology, 69(2), 230–240. doi:10.1007/s00244-015-0163-6.
Bulut, S., Akkaya, L., Gok, V., & Konuk, M. (2011). Organochlorine pesticide (OCP) residues in cow’s, buffalo’s, and sheep’s milk from Afyonkarahisar region, Turkey. Environmental Monitoring and Assessment, 181(1–4), 555–562. doi:10.1007/s10661-010-1849-x.
Chen, X., Panuwet, P., Hunter, R. E., Riederer, A. M., Bernoudy, G. C., Barr, D. B., et al. (2014). Method for the quantification of current use and persistent pesticides in cow milk, human milk and baby formula using gas chromatography tandem mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 970, 121–130. doi:10.1016/j.jchromb.2014.08.018.
Commission, C. A. (2013). Pesticide residues in food and feed by the and FAO/WHO Food standards, Codex Alimentarius.
Corcellas, C., Feo, M. L., Torres, J. P., Malm, O., Ocampo-Duque, W., Eljarrat, E., et al. (2012). Pyrethroids in human breast milk: occurrence and nursing daily intake estimation. Environment International, 47, 17–22.
Deti, H., Hymete, A., Bekhit, A. A., Mohamed, A. M. I., & Bekhit, A. E.-D. A. (2014). Persistent organochlorine pesticides residues in cow and goat milks collected from different regions of Ethiopia. Chemosphere, 106, 70–74. doi:10.1016/j.chemosphere.2014.02.012.
Economic Survey of Pakistan. (2006). Finance division. Islamabad: Government of Pakistan.
Fang, G., Lau, H. F., Law, W. S., & Li, S. F. Y. (2012). Systematic optimisation of coupled microwave-assisted extraction-solid phase extraction for the determination of pesticides in infant milk formula via LC–MS/MS. Food Chemistry, 134(4), 2473–2480. doi:10.1016/j.foodchem.2012.04.076.
FAO/WHO Food Standards Programme, C. A. C. (2008). Report of the fortieth session of the Codex Committee on Pesticide Residues. Hangzhou, China.
Goodarzi, M., Ortiz, E. V., Coelho, L. D. S., & Duchowicz, P. R. (2010). Linear and non-linear relationships mapping the Henry’s law parameters of organic pesticides. Atmospheric Environment, 44(26), 3179–3186. doi:10.1016/j.atmosenv.2010.05.025.
Iftikhar, B., Siddiqui, S., & Rehman, S. (2014). Assessment of the dietary transfer of pesticides to dairy milk and its effect on human health. African Journal of Biotechnology, 13(3), 476–485.
John, P. J., Bakore, N., & Bhatnagar, P. (2001). Assessment of organochlorine pesticide residue levels in dairy milk and buffalo milk from Jaipur City, Rajasthan, India. Environment International, 26(4), 231–236. doi:10.1016/S0160-4120(00)00111-2.
Kalwar, N. H., Nafady, A., Sirajuddin, Sherazi, S. T. H., Soomro, R. A., Hallam, K. R., et al. (2015). Catalytic degradation of imidacloprid using L-serine capped nickel nanoparticles. Materials Express, 5(2), 121–128. doi:10.1166/mex.2015.1224.
Kampire, E., Kiremire, B. T., Nyanzi, S. A., & Kishimba, M. (2011). Organochlorine pesticide in fresh and pasteurized cow’s milk from Kampala markets. Chemosphere, 84(7), 923–927. doi:10.1016/j.chemosphere.2011.06.011.
Khan, M., Mahmood, H. Z., & Damalas, C. A. (2015). Pesticide use and risk perceptions among farmers in the cotton belt of Punjab, Pakistan. Crop Protection, 67, 184–190. doi:10.1016/j.cropro.2014.10.013.
Khwaja, S., Mushtaq, R., Mushtaq, R., Yousuf, M., Attaullah, M., Tabbassum, F., et al. (2013). Monitoring of biochemical effects of organochlorine pesticides on human health. Health, 05(8), 9. doi:10.4236/health.2013.58182.
Koesukwiwat, U., Lehotay, S. J., Miao, S., & Leepipatpiboon, N. (2010). High throughput analysis of 150 pesticides in fruits and vegetables using QuEChERS and low-pressure gas chromatography-time-of-flight mass spectrometry. Journal of Chromatography A, 1217(43), 6692–6703. doi:10.1016/j.chroma.2010.05.012.
Lehotay, S. J. (2007). Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study. Journal of AOAC International, 90(2), 485–520.
Lehotay, S. J., Mastovska, K., Lightfield, A. R., & Gates, R. A. (2010a). Multi-analyst, multi-matrix performance of the QuEChERS approach for pesticide residues in foods and feeds using HPLC/MS/MS analysis with different calibration techniques. Journal of AOAC International, 93(2), 355–367.
Lehotay, S. J., Son, K. A., Kwon, H., Koesukwiwat, U., Fu, W., Mastovska, K., et al. (2010b). Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A, 1217(16), 2548–2560. doi:10.1016/j.chroma.2010.01.044.
Luzardo, O. P., Almeida-Gonzalez, M., Henriquez-Hernandez, L. A., Zumbado, M., Alvarez-Leon, E. E., & Boada, L. D. (2012). Polychlorobiphenyls and organochlorine pesticides in conventional and organic brands of milk: occurrence and dietary intake in the population of the Canary Islands (Spain). Chemosphere, 88(3), 307–315. doi:10.1016/j.chemosphere.2012.03.002.
Martins, J. G., Amaya Chávez, A., Waliszewski, S. M., Colín Cruz, A., & García Fabila, M. M. (2013). Extraction and clean-up methods for organochlorine pesticides determination in milk. Chemosphere, 92(3), 233–246. doi:10.1016/j.chemosphere.2013.04.008.
Muhammad, F., Javed, I., Akhtar, M., Zia-ur-Rahman, Awais, M. M., Saleemi, M. K., et al. (2012). Quantitative structure activity relationship and risk analysis of some pesticides in the cattle milk. Pakistan Veterinary Journal, 32(4), 589–592.
Muhammad, F., Awais, M. M., Akhtar, M., & Anwar, M. I. (2013). Quantitative structure activity relationship and risk analysis of some pesticides in the goat milk. Iranian Journal of Environmental Health Science & Engineering, 10(1), 4.
Muñoz-Quezada, M. T., Lucero, B. A., Barr, D. B., Steenland, K., Levy, K., Ryan, P. B., et al. (2013). Neurodevelopmental effects in children associated with exposure to organophosphate pesticides: a systematic review. Neurotoxicology, 39, 158–168. doi:10.1016/j.neuro.2013.09.003.
Pagliuca, G., Serraino, A., Gazzotti, T., Zironi, E., Borsari, A., & Rosmini, R. (2006). Organophosphorus pesticides residues in Italian raw milk. The Journal of Dairy Research, 73(3), 340–344. doi:10.1017/s0022029906001695.
Rejeb, S. B., Cléroux, C., Lawrence, J. F., Geay, P.-Y., Wu, S., & Stavinski, S. (2001). Development and characterization of immunoaffinity columns for the selective extraction of a new developmental pesticide: thifluzamide, from peanuts. Analytica Chimica Acta, 432(2), 193–200. doi:10.1016/S0003-2670(00)01376-3.
Rissato, S. R., Galhiane, M. S., Apon, B. M., & Arruda, M. S. (2005). Multiresidue analysis of pesticides in soil by supercritical fluid extraction/gas chromatography with electron-capture detection and confirmation by gas chromatography–mass spectrometry. Journal of Agricultural and Food Chemistry, 53(1), 62–69. doi:10.1021/jf048772s.
Sanagi, M. M., Salleh, S., Ibrahim, W. A. W., Naim, A. A., Hermawan, D., Miskam, M., et al. (2013). Molecularly imprinted polymer solid-phase extraction for the analysis of organophosphorus pesticides in fruit samples. Journal of Food Composition and Analysis, 32(2), 155–161. doi:10.1016/j.jfca.2013.09.001.
Sapahin, H. A., Makahleh, A., & Saad, B. (2014). Determination of organophosphorus pesticide residues in vegetables using solid phase micro-extraction coupled with gas chromatography-flame photometric detector. Arabian Journal of Chemistry. doi:10.1016/j.arabjc.2014.12.001.
Serrano, R., Blanes, M. A., & López, F. J. (2008). Biomagnification of organochlorine pollutants in farmed and wild gilthead sea bream (Sparus aurata) and stable isotope characterization of the trophic chains. Science of the Total Environment, 389(2–3), 340–349. doi:10.1016/j.scitotenv.2007.09.020.
Shahzadi, N., Imran, M., Sarwar, M., Hashmi, A. S., & Wasim, M. (2013). Identification of pesticides residues in different samples of milk. Journal of Agroalimentary Processes and Technologies, 19(2), 167–172.
Stocka, J., Tankiewicz, M., Biziuk, M., & Namieśnik, J. (2011). Green aspects of techniques for the determination of currently used pesticides in environmental samples. International Journal of Molecular Sciences, 12(11), 7785–7805. doi:10.3390/ijms12117785.
Sun, H., Ge, X., Lv, Y., & Wang, A. (2012). Application of accelerated solvent extraction in the analysis of organic contaminants, bioactive and nutritional compounds in food and feed. Journal of Chromatography, A, 1237, 1–23. doi:10.1016/j.chroma.2012.03.003.
Tariq, M. I., Afzal, S., Hussain, I., & Sultana, N. (2007). Pesticides exposure in Pakistan: a review. Environment International, 33(8), 1107–1122. doi:10.1016/j.envint.2007.07.012.
Tsiplakou, E., Anagnostopoulos, C. J., Liapis, K., Haroutounian, S. A., & Zervas, G. (2010). Pesticides residues in milks and feedstuff of farm animals drawn from Greece. Chemosphere, 80(5), 504–512. doi:10.1016/j.chemosphere.2010.04.069.
Zheng, G., Han, C., Liu, Y., Wang, J., Zhu, M., Wang, C., et al. (2014). Multiresidue analysis of 30 organochlorine pesticides in milk and milk powder by gel permeation chromatography-solid phase extraction-gas chromatography-tandem mass spectrometry. Journal of Dairy Science, 97(10), 6016–6026. doi:10.3168/jds.2014-8192.
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Jawaid, S., Talpur, F.N., Nizamani, S.M. et al. Multipesticide residue levels in UHT and raw milk samples by GC-μECD after QuEChER extraction method. Environ Monit Assess 188, 230 (2016). https://doi.org/10.1007/s10661-016-5222-6
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DOI: https://doi.org/10.1007/s10661-016-5222-6