Abstract
Bacteria holding-time experiments of up to 62 h were performed on five surface-water samples from four urban stream sites in the vicinity of Atlanta, GA, USA that had relatively high densities of coliform bacteria (Escherichia coli densities were all well above the US Environmental Protection Agency criterion of 126 colonies (100 ml) − 1 for recreational waters). Holding-time experiments were done for fecal coliform using the membrane filtration modified fecal coliform (mFC) agar method and for total coliform and E. coli using the Colilert®-18 Quanti-Tray® method. The precisions of these analytical methods were quantified. Precisions determined for fecal coliform indicated that the upper bound of the ideal range of counts could reasonably be extended upward and would improve precision. For the Colilert®-18 method, analytical precisions were similar to the theoretical precisions for this method. Fecal and total coliform densities did not change significantly with holding times up to about 27 h. Limited information indicated that fecal coliform densities might be stable for holding times of up to 62 h, whereas total coliform densities might not be stable for holding times greater than about 27 h. E. coli densities were stable for holding times of up to 18 h—a shorter period than indicated from a previous studies. These results should be applicable to non-regulatory monitoring sampling designs for similar urban surface-water sample types.
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References
Clesceri, L. S., Greenberg, A. E., & Eaton, A. D. (Eds.). (1998). Standard methods for the examination of water and wastewater (20th ed.). Washington, D.C.: American Public Health Association-American Water Works Association-Water Environment Federation (pp. 9.21, 9.63–65, 9.69–70).
Dutka, B. J., & El-Shaarawi, A. (1980). Microbiological water and effluent sample preservation. Canadian Journal of Microbiology, 26(8), 921–929.
Ferguson, C. M. (1994). Refrigerated autosampling for the assessment of bacteriological water quality. Water Research, 28(4), 841–847. doi:10.1016/0043-1354(94)90090-6.
Francy, D. S., & Darner, R. A. (2003). Forecasting bacteria levels at bathing beaches in Ohio. US Geological Survey Fact Sheet FS-132-02 (4 pp.). Reston: USGS.
Francy, D. S., Gifford, A. M., & Darner, R. A. (2002). Escherichia coli at Ohio bathing beaches–distribution, sources, wastewater indicators, and predictive modeling. US Geological Survey Water-Resources Investigations Report 02-4285 (120 pp.). Reston: USGS.
Horowitz, A. J. (2006). The US Geological Survey and City of Atlanta water-quality and watery-quantity monitoring network. U.S. Geological Survey Fact Sheet 2005-3126 (4 pp.). Reston: USGS.
Hurley, M. A., & Roscoe, M. E. (1983). Automated statistical analysis of microbial enumeration by dilution series. The Journal of Applied Bacteriology, 55(1), 159–164.
Idexx Laboratories, Incorporated (2002). Colilert ® -18 test kit. http://www.idexx.com/water/refs/060202711C18.pdf. Accessed 6 June 2007.
Lawrence, S. J., & Pojunas, T. K. (2003). Preliminary results from a cooperative program for monitoring and disseminating bacteria data. In Proceedings of achieving sustainable water resources in areas experiencing rapid population growth. 2003 American Institute of Hydrology Annual Meeting and Conference held October 19–22, 2003, Atlanta, Georgia.
Myers, D. N. (2004). Fecal indicator bacteria (3rd ed., version 1.2): US Geological Survey Techniques of Water-Resources Investigations, book 9, chap. A7. section 7.1, November 2004. http://pubs.water.usgs.gov/twri9A7/. Accessed 6 June 2007.
Pope, M. L., Bussen, M., Feige, M. A., Shacix, L., Gonder, S., Rogers, C., et al. (2003). Assessment of the effects of holding time and temperature on Escherichia coli densities in surface water samples. Applied and Environmental Microbiology, 69(10), 6201–6207. doi:10.1128/AEM.69.10.6201-6207.2003.
Selvakumar, A., Borst, M., Boner, M., & Mallon, P. (2004). Effects of sample holding time on concentrations of microorganisms in water samples. Water Environment Research, 76(1), 67–72. doi:10.2175/106143004X141591.
Standridge, J. H., & Lesar, D. J. (1977). Comparison of four-hour and twenty-four-hour refrigerated storage of nonpotable water for fecal coliform analysis. Applied and Environmental Microbiology, 34(4), 398–402.
Taylor, J. K. (1987). Quality assurance of chemical measurements (328 pp.). Chelsea: Lewis.
US Congress (2000). Beaches environmental assessment and coastal health act of 2000. Washington, D.C.: US Congress, Public Law 106-284, October 10, 2000, 114,870-877.
US Environmental Protection Agency (1986). Ambient water quality criteria for bacteria—1986 (18 pp.). Washington, D.C.: USEPA, EPA440/5-84-002, January 1986.
US Environmental Protection Agency (2002). List of drinking water contaminants and MCLs. Washington, D.C.: USEPA, EPA 816-F-02-013.
US Environmental Protection Agency (2003a). Bacterial water quality standards for recreational waters (Freshwater and marine waters): Status report (28 pp.). Washington, D.C.: USEPA, EPA-823-R-03-008, June 2003.
US Environmental Protection Agency (2003b). Guidelines establishing test procedures for the analysis of pollutants; analytical methods for biological pollutants in ambient water; final rule. Federal Register, 68, 43272–43283.
US Environmental Protection Agency (2004). Water quality standards for coastal and Great lakes recreation waters. Federal Register, 69, 67218–67243.
Woodworth, M. T., & Connor, B. F. (2003). Results of the US Geological Survey’s analytical evaluation program for standard reference samples distributed in March 2003 (109 pp.). Reston: USGS, US Geological Survey Open-File Report 03-261.
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Aulenbach, B.T. Bacteria holding times for fecal coliform by mFC agar method and total coliform and Escherichia coli by Colilert®-18 Quanti-Tray® method. Environ Monit Assess 161, 147–159 (2010). https://doi.org/10.1007/s10661-008-0734-3
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DOI: https://doi.org/10.1007/s10661-008-0734-3