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Occurrence of the genus Dolichodorus Cobb 1914 (Nematoda: Dolichodoridae) in Iran, and description of D. rex n. sp.

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Abstract

The genus Dolichodorus was discovered from a natural forest in Gilan province, north Iran, in association with an oak tree. The recovered population represents a new species, and was described and illustrated based upon morphological and molecular data, as D. rex n. sp. It is characterized by 2171–3275 μm long females having offset crown-like cephalic region with well sclerotized cephalic framework, raised perioral disc apparently with a small raised oral plate, 118.5, 130.0 μm long stylet, its conus ca. 55% of the total length and three posteriorly sloping knobs, 291–316 μm long pharynx with a short overlap, secretory-excretory pore at slightly variable position (one-two annuli posterior, or four annuli anterior to metacorpus base), vulva sunken in body, at %50 of the body length, vagina heavily sclerotized with two drop-like refractive pieces close to the vulval opening, 78–104 μm long tail with proximal convex conoid, and conical distal part with pointed to blunt tip; and a 2634 μm long male with 108.5 μm long stylet, 291 μm long pharynx, 20 μm long tail and 53 μm long spicules. In molecular phylogenetic analyses using partial sequences of the small, and D2-D3 expansion segments of the large subunit ribosomal DNA, the new species formed a clade with two other currently sequenced species of the genus in both phylogenies.

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Notes

  1. The species epithet is the Latin translation of king, referring to the specific cephalic region shape of the new species, resembling a crown.

References

  • Andrássy, I. (1976). Evolution as a basis for the systematization of nematodes (p. 288). London: Pitman Publishing.

    Google Scholar 

  • Chitwood, B. G. (1950). General structure of nematodes. In B. G. Chitwood & M. B. Chitwood (Eds.), Introduction to nematology. Section 1, anatomy (pp. 7–27). Baltimore: Monumental printing Co.

  • Cobb, N. A. (1914). The north American free- living fresh water nematodes. Contributions to a science of hematology II. Transactions of American Microscopical Society, 33, 69–134.

    Article  Google Scholar 

  • De Grisse, A. T. (1969). Redescription ou modifications de quelques techniques utilisées dans l’étude des nematodes phytoparasitaires. Mededelingen Rijksfakulteiteit Landbouwwetenschappen, Gent, 34, 351–369.

  • de Man, J. G. (1884). Die frei in der reinen Erde und im süssen Wasser lebenden Nematoden der niederländischen Fauna (pp. 1–206). Leiden.

  • Dorris, M., Viney, M. E., & Blaxter, M. L. (2002). Molecular phylogenetic analysis of the genus Strongyloides and related nematodes. International Journal for Parasitology, 32, 1507–1517.

    Article  CAS  Google Scholar 

  • Esquivel, A., Ferris, H., Cid del Prado, I., & Subbotin, S. A. (2017). Dolichodorus costaricensis n. sp. (Tylenchida: Dolichodoridae): A new awl nematode species from the Central Pacific region of Costa Rica. Nematropica, 47, 143–154.

    Google Scholar 

  • Geraert, E. (2011). The Dolichodoridae of the World: Identification of the family Dolichodoridae (Nematoda) (p. 520). Ghent: Academic Press.

  • Ghaderi, R., Kashi, L., & Karegar, A. (2018). Plant-parasitic nematodes in Iran (p. 756). Science reference in collaboration with the Iranian Society of Nematology. (In Persian).

  • Gharahkhani, A., Pourjam, E., & Pedram, M. (2019). Occurrence of Neodolichodorus persiangulfus n. sp. (Nematoda: Dolichodoridae) in mangrove forests of southern Iran. Forest Pathology, 49, e12563.

    Article  Google Scholar 

  • Golden, A. M., Handoo, Z. A., & Wehunt, E. J. (1986). Description of Dolichodorus cobbi n. sp. (Nematoda: Dolichodoridae) with morphometrics and lectotype designation of D. heterocephalus Cobb, 1914. Journal of Nematology, 18, 556–562.

    PubMed  PubMed Central  CAS  Google Scholar 

  • Guirado, D. J., Navarro, R. M. M., Liébanas, G., Landa, B. B., & Castillo, P. (2007). Morphological and molecular characterisation of a new awl nematode, Dolichodorus mediterraneus sp. n. (Nematoda: Dolichodoridae), from Spain. Nematology, 9, 189–199.

    Article  Google Scholar 

  • Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J., & Helder, J. (2006). Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution, 23, 1792–1800.

    Article  CAS  Google Scholar 

  • Holterman, M., Rybarczyk, K., van den Elsen, S., van Megen, H., Mooyman, P., Peña Santiago, R., Bongers, T., Bakker, J., & Helder, J. (2008). A ribosomal DNA-based framework for the detection and quantification of stress-sensitive nematode families in terrestrial habitats. Molecular Ecology Resources, 8, 23–34.

    Article  CAS  Google Scholar 

  • Huson, D. H., & Scornavacca, C. (2012). Dendroscope 3: An interactive tool for rooted phylogenetic trees and networks. Systematic Biology, 61, 1061–1067.

    Article  Google Scholar 

  • Jenkins, W. (1964). A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporter, 48, 692.

    Google Scholar 

  • Katoh, K., & Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution, 30, 772–780.

    Article  CAS  Google Scholar 

  • Kiontke, K., Gavin, N. P., Raynes, Y., Roehrig, C., Piano, F., & Fitch, D. H. A. (2004). Caenorhabditis phylogeny predicts convergence of hermaphroditism and extensive intron loss. Proceedings of the National Academy of Sciences of the United States of America, 101, 9003–9008.

  • Larget, B., & Simon, D. L. (1999). Markov chain Monte Carlo algorithms for the Bayesian analysis of phylogenetic trees. Molecular Biology and Evolution, 16, 750–759.

    Article  CAS  Google Scholar 

  • Mullin, P., Harris, T., & Powers, T. (2005). Phylogenetic relationships of Nygolaimina and Dorylaimina (Nematoda: Dorylaimida) inferred from small subunit ribosomal DNA sequences. Nematology, 7, 59–79.

    Article  CAS  Google Scholar 

  • Nunn, G. B. (1992). Nematode molecular evolution. Ph.D. Thesis, University of Nottingham, Nottingham, UK.

  • Nylander, J. A. A. (2004). MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre: Uppsala University.

  • Oliveira, R. D. L., Santin, Â. M., Seni, D. J., Dietrich, A., Salazar, L. A., Subbotin, S. A., Mundo-Ocampo, M., Goldenberg, R., & Barreto, R. W. (2013). Ditylenchus gallaeformans sp. n. (Tylenchida: Anguinidae) a neotropical nematode with biocontrol potential against weedy Melastomataceae. Nematology, 15, 179–196.

    Article  Google Scholar 

  • Örley, L. (1880). Monograph of the Anguillulids. Term. Füz, 4, 16–150.

    Google Scholar 

  • Pedram, M. (2017). Cryptaphelenchus varicaudatus n. sp. (Rhabditida: Ektaphelenchinae) from Tehran Province, Iran. Journal of Nematology, 49, 223.

    Article  Google Scholar 

  • Pereira, T. J., Qing, X., Chang, K. F., Mundo-Ocampo, M., Cares, J. E., Ragsdale, E. J., Nguyen, C. N. & Baldwin, J. G. (2017). Phylogeny and biogeography of the genus Cephalenchus (Tylenchomorpha, Nematoda). Zoologica Scripta, 46, 506–20.

  • Rambaut, A., & Drummond, A. J. (2009). Tracer version 1.5 [computer program].

  • Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    Article  CAS  Google Scholar 

  • Siddiqi, M. R. (2000). Tylenchida Parasites of Plants and Insects (2nd ed.p. 833). Wallingford: UK, CABI publishing.

  • Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.

    Article  CAS  Google Scholar 

  • Taylor, A. L. (1936). The genera and species of the Criconematinae, a sub-family of the Anguillulinidae (Nematoda). Transactions of the American Microscopical Society, 55, 391–421.

    Article  Google Scholar 

  • Whitehead, A. G., & Hemming, J. R. (1965). A comparison of some quantitative methods for extracting small vermiform nematodes from soil. Annals of Applied Biology, 55, 25–38.

    Article  Google Scholar 

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Acknowledgements

The studied soil samples in present study were kindly provided by Dr. Reyhaneh Gholami. This help is appreciated. We thank Dr. Hossein Shafizadeh Moghaddam for his technical helps, and Tarbiat Modares University and Iranian National Science foundation (INSF) for financial supports.

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Authors and Affiliations

  1. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Samira Fadakar & Majid Pedram

  2. Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

    Farahnaz Jahanshahi Afshar

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  1. Samira Fadakar
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  2. Farahnaz Jahanshahi Afshar
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  3. Majid Pedram
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Correspondence to Majid Pedram.

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All the authors certify that they do not have any conflict of interest, the presented data are original and the presented data are not published, and are not under evaluation for publication elsewhere, all prevailing local, national and international regulations and conventions, and normal scientific ethical practices, have been respected. They also certify that the final version of this manuscript has been reviewed by all authors.

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The soil samples analyzed in this study were collected from the natural public regions that are not under protection against soil samplings.

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The authors certify that they have accepted the principles of ethical standards and that guidelines of professional conduct have been followed. The funders had no role in designing of this study, collecting and analyzing of the data, preparing the manuscript and are not involved in evaluating or publishing process of this research.

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Fadakar, S., Jahanshahi Afshar, F. & Pedram, M. Occurrence of the genus Dolichodorus Cobb 1914 (Nematoda: Dolichodoridae) in Iran, and description of D. rex n. sp.. Eur J Plant Pathol 158, 443–455 (2020). https://doi.org/10.1007/s10658-020-02084-9

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  • DOI: https://doi.org/10.1007/s10658-020-02084-9

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