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Variable ITS-copy number at different developmental stages of Meloidogyne hapla and M. chitwoodi

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Abstract

Quantitative polymerase chain reaction (qPCR) can be used for the accurate quantification of plant-parasitic nematodes from soil samples. Unlike the traditional Baermann funnel and centrifugal flotation techniques followed by visual enumeration, all developmental stages of plant-parasitic nematodes can be detected and quantified by using qPCR. However, little is known about the amount of DNA between different stages of plant-parasitic nematodes. Here, we show that ITS-copy number varies with developmental stage of Meloidogyne chitwoodi and M. hapla. The number of copies of ITS was lower in cell-stage eggs (51 ± 7.4 for M. hapla and 31 ± 6.0 for M. chitwoodi) than in second-stage juveniles (1345 ± 130.6 for M. hapla and 1036 ± 140.3 for M. chitwoodi) and females (19,508 ± 3367.3 for M. hapla and 9049 ± 316.7 for M. chitwoodi).

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Acknowledgments

The authors are grateful for FERA (The Food and Environment Research Agency, United Kingdom) for providing nematode inoculum. E.A. Lopes thanks CNPq (Proc. 304663/2014-0) and The James Hutton Institute for Post-Doctoral Training.

Funding

This study was funded by CNPq (PDE 233650/2014–8) and the Rural and Environment Science and Analytical Services (RESAS) Division of the Scottish Government.

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Authors and Affiliations

  1. Universidade Federal de Viçosa, Campus Rio Paranaíba, PO Box 22, Rio Paranaíba, 38810-000, Brazil

    Everaldo Antônio Lopes

  2. The James Hutton Institute, Invergowrie, Dundee, Scotland, DD2 5DA, UK

    David Mark Roberts & Vivian Carol Blok

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  1. Everaldo Antônio Lopes
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  2. David Mark Roberts
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Correspondence to Everaldo Antônio Lopes.

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Lopes, E.A., Roberts, D.M. & Blok, V.C. Variable ITS-copy number at different developmental stages of Meloidogyne hapla and M. chitwoodi. Eur J Plant Pathol 154, 843–848 (2019). https://doi.org/10.1007/s10658-019-01672-8

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