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A proteomics survey on wheat susceptibility to Fusarium head blight during grain development

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Abstract

The mycotoxigenic fungal species Fusarium graminearum is able to attack several important cereal crops, such as wheat and barley. By causing Fusarium Head Blight (FHB) disease, F. graminearum induces yield and quality losses and poses a public health concern due to in planta mycotoxin production. The molecular and physiological plant responses to FHB, and the cellular biochemical pathways used by F. graminearum to complete its infectious process remain still unknown. In this study, a proteomics approach, combining 2D-gel approach and mass spectrometry, has been used to determine the specific protein patterns associated with the development of the fungal infection during grain growth on susceptible wheat. Our results reveal that F. graminearum infection does not deeply alter the grain proteome and does not significantly disturb the first steps of grain ontogeny but impacts molecular changes during the grain filling stage (impact on starch synthesis and storage proteins). The differentially regulated proteins identified were mainly involved in stress and defence mechanisms, primary metabolism, and main cellular processes such as signalling and transport. Our survey suggests that F. graminearum could take advantage of putative susceptibility factors closely related to grain development processes and thus provide new insights into key molecular events controlling the susceptible response to FHB in wheat grains.

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Acknowledgments

We thank members of the Experimental Unit INRA 1375 PHACC (INRA Center of Clermont-Theix, France) for plants production, Christophe Chambon and Didier Viala from PFEM (INRA Center of Clermont-Theix, France) for mass spectrometry analyses. This study is part of CC PhD work, funded by the French National Institute for Agronomic Research (INRA).

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Authors and Affiliations

  1. INRA, UMR 1095, Genetics, Diversity and Ecophysiology of Cereals, F‐63100, Clermont-Ferrand, France

    Cherif Chetouhi, Ludovic Bonhomme, Philippe Lecomte, Florence Cambon, Marielle Merlino & Thierry Langin

  2. UBP, UMR Genetics, Diversity and Ecophysiology of Cereals, F‐63100, Clermont-Ferrand, France

    Cherif Chetouhi, Ludovic Bonhomme, Philippe Lecomte, Florence Cambon, Marielle Merlino & Thierry Langin

  3. Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement, Clermont-Ferrand, France; CNRS, UMR 6023, LMGE, Aubière, France

    David Georges Biron

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  1. Cherif Chetouhi
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  2. Ludovic Bonhomme
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  3. Philippe Lecomte
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Correspondence to Ludovic Bonhomme or Thierry Langin.

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Chetouhi, C., Bonhomme, L., Lecomte, P. et al. A proteomics survey on wheat susceptibility to Fusarium head blight during grain development. Eur J Plant Pathol 141, 407–418 (2015). https://doi.org/10.1007/s10658-014-0552-0

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