Abstract
Brassica rapa (chinese cabbage) is one of the main vegetable crops grown in Asian countries. Pectobacterium carotovorum subsp. carotovorum causes severe economic loss in this crop as well as in other Brassica crops through soft rot disease. Cysteine proteases like bromelain, papain or ficin show toxic effects to herbivorous insects and pathogenic bacteria. They have been known to be critical factors in plant defence mechanisms. The current study investigated the effect of bromelain gene (BL1) of pineapple (Ananas comosus L. Merrill) on enhancing resistance to soft rot in transgenic Brassica rapa ‘Seoulbaechu’. Three homozygous T2 lines were inoculated with Pectobacterium carotovorum subsp. carotovorum and BL8-2 line showed the lowest rate of infected leaves (RIL) in both wound inoculation and non-wound inoculation, when the non-infected line showed 100 % RIL in both cases. The highest expression of BL1 gene was also observed in BL8-2 homozygous line. Thus, the over-expressed BL1 gene conferred enhanced resistance to soft rot in Brassica rapa.
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Ahmed, N. U., Park, J. I., Seo, M. S., Kumar, T. S., Lee, I. H., Park, B. S., & Nou, I. S. (2012). Identification and expression analysis of chitinase genes related to biotic stress resistance in Brassica. Molecular Biology Reports, 39, 3649–3657.
Batkin, S., Taussig, S. J., & Szekerezes, J. (1988). Antimetastatic effect of bromelain with or without its proteolytic and anticoagulant activity. Journal of Cancer Research and Clinical Oncology, 114, 507–508.
Fritz, V. A., & Honma, S. (1987). The effect of raised beds, population densities, and planting date on the incidence of bacterial in Chinese cabbage. Journal of the American Society for Horticultural Science, 112, 41–44.
Garbin, F., Harrach, T., Eckert, K., & Maurer, H. R. (1994). Bromelain proteinase F9 augments human lymphocyte-mediated growth inhibition of various tumor cells in vitro. International Journal of Oncology, 5, 197–203.
Gaspani, L., Limiroli, E., Ferrario, P., & Bianchi, M. (2002). In vivo and in vitro effects of bromelain on PGE2 and SP concentrations in the inflammatory exudate in rats. Pharmacology, 65, 83–86.
Grabowska, E., Eckert, K., Fichtner, I., Schulze-Foster, K., & Maurer, H. R. (1997). Bromelain proteases suppresses growth, invasion and lung metastasis of B16F10 mouse melanoma cells. International Journal of Oncology, 11, 243–248.
Grudkowska, M., & Zagdańska, B. (2004). Multifunctional role of plant cysteine proteinases. Acta Biochemica Polonica, 51(3), 609–624.
Hwang, B. H., Bae, H., Lim, H. S., Kim, K. B., Kim, S. J., Im, M. H., Park, B. S., Kim, D. S., & Kim, J. (2010). Overexpression of polygalacturonase-inhibiting protein 2 (PGIP2) of Chinese cabbage (Brassica rapa ssp. pekinensis) increased resistance to the bacterial pathogen Pectobacterium carotovorum ssp. carotovorum. Plant Cell Tissue and Organ Culture, 103, 293–305.
Jung, Y. J., Hyun, K. G., Choi, J. S., Lee, S. Y., Nou, I. S., Park, J. H., & Kang, K. K. (2006). Characterization of transgenic Lettuce (Lactuca sativa L.) using a BL1 gene encoding bromelain isolated from pineapple. Korean Journal of Plant Biotechnology, 33(1), 27–32.
Jung, Y. J., Choi, C. S., Park, J. H., Kang, H. W., Choi, J. E., Nou, I. S., Lee, S. Y., & Kang, K. K. (2008). Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot. Electronic Journal of Biotechnology, 11(1).
Konno, K., Hirayama, C., Nakamura, M., Tateishi, K., Tamura, Y., Hattori, M., & Kohno, K. (2004). Papain protects papaya trees from herbivorous insects: role of cysteine proteases in latex. The Plant Journal, 37, 370–378.
Li, S. D. (1995). Progress in disease resistant breed of main vegetables (pp. 96–100). Beijing: Science Press.
Linke, C., Conrath, U., Jeblick, W., Betsche, T., Mahn, A., Düring, K., & Neuhaus, H. E. (2002). Inhibition of the plastidic ATP/ADP transporter protein primes potato tubers for augmented elicitation of defense responses and enhances their resistance against Erwinia carotovora. Plant Physiology, 129(4), 1607–1615.
Luerti, M., & Vignali, M. L. (1978). Influence of bromelain on penetration of antibiotics in uterus, salpinx and ovary. Drugs under Experimental and Clinical Research, 4(1), 45–48.
Maurer, H. R. (2001). Bromelain: biochemistry, pharmacology and medical use. Cellular and Molecular Life Sciences, 58(9), 1234–1245.
Mynott, T. L., Crossett, B., & Prathalingam, S. R. (2002). Proteolytic inhibition of Salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases ERK and JNK in cultured human intestinal cells. Infection and Immunity, 70(1), 86–95.
Orsini, R. A. (2006). Bromelain. Plastic and Reconstructive Surgery, 118(7), 1640–1644.
Ren, J., Petzoldt, R., & Dickson, M. H. (2001). Genetics and population improvement of resistance to bacterial soft rot in Chinese cabbage. Euphytica, 117, 197–207.
Segura, A., Moreno, M., Madueno, F., Molina, A., & Garcia-Olmedo, F. (1999). Snakin-1, a peptide from potato that is active against plant pathogens. Molecular Plant-Microbe Interactions, 12, 16–23.
Takasaki, T., Hatakeyama, K., Ojima, K., Watanabe, M., Toriyama, K., & Hinata, K. (1997). Factors influencing Agrobacterium-mediated transformation of Brassica rapa L. Breeding Science, 47, 127–134.
Tinozzi, S., & Venegoni, A. (1978). Effect of bromelain on serum and tissue levels of amoxycillin. Drugs under Experimental and Clinical Research, 4, 39–44.
Toth, I. K., Newton, J. A., Hyman, L. J., Lees, A. K., Daykin, M., Ortori, C., Williams, P., & Fray, R. G. (2004). Potato plants genetically modified to produce N-acylhomoserine lactones increase susceptibility to soft rot erwiniae. Molecular Plant-Microbe Interactions, 17(8), 880–887.
Vanjildorj, E., Song, S. Y., Yang, Z. H., Choi, J. E., Noh, Y. S., Park, S., Lim, W. J., Cho, K. M., Han Dae Yun, H. D., & Lim, Y. P. (2009). Enhancement of tolerance to soft rot disease in the transgenic Chinese cabbage (Brassica rapa L. ssp. pekinensis) inbred line, Kenshin. Plant Cell Reports, 28, 1581–1591.
Wigoda, N., Ben-Nissan, G., Granot, D., Schwartz, A., & Weiss, D. (2006). The gibberellin-induced, cysteine-rich protein GIP2 from Petunia hybrida exhibits in planta antioxidant activity. The Plant Journal, 48, 796–805.
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This research was supported by iPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.
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Young-Jin Koh and Jong-In Park equally contributed to this work.
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Koh, YJ., Park, JI., Ahmed, N.U. et al. Enhancement of resistance to soft rot (Pectobacterium carotovorum subsp. carotovorum) in transgenic Brassica rapa . Eur J Plant Pathol 136, 317–322 (2013). https://doi.org/10.1007/s10658-012-0165-4
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DOI: https://doi.org/10.1007/s10658-012-0165-4