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Genetic diversity analysis of Acidovorax citrulli in China

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Abstract

Acidovorax citrulli has become quite common in China. A collection of 118 strains of A. citrulli was made from throughout China and other countries to determine their genetic relatedness. Strains were identified as A. citrulli by pathogenicity, phenotypic characterization, and PCR. Genetic diversity was determined using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). PFGE electrophoresis resulted in nine genotypes, which could be typed into two groups based on host; group 1 included strains mainly from melon and group 2 included strains mainly from watermelon. MLST analysis resulted in 73 sequence types (ST) among the 118 A. citrulli strains. All A. citrulli strains were typed into three groups: group 1 with 82 strains (including type strain Fc247), group 2 with 35 strains and group 3 a singleton (Fc380). Similar to PFGE results, group 1 included strains mainly from melon and group 2 included strains mainly from watermelon. The difference was the 10 watermelon strains (pslbtw1-3, 5–11) from Beijing grouped with melon strains of group 1 based on MLST, suggesting that these 10 watermelon strains had a close relationship with melon. Our study indicated that there was genetic differentiation among A. citrulli strains between watermelon and melon. Also, our study was the first attempt to compare PFGE and MLST on analyzing genetic diversity of A. citrulli strains and proved MLST could better distinguish A. citrulli strains.

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References

  • Aanensen, D. M., & Spratt, B. G. (2005). The multilocus sequence typing network: mlst.net. Nucleic Acids Research, 33, 728–733.

    Article  Google Scholar 

  • Almeida, N. F., Yan, S. C., Cai, R., et al. (2010). PAMDB, a multilocus sequence typing and analysis database and website for plant-associated microbes. Phytopathology, 100, 208–215.

    Article  PubMed  CAS  Google Scholar 

  • Burdman, S., Kots, N., Kritzman, G., et al. (2005). Molecular, physuological, and host-range characterization of Acidovorax avenae subsp. citrulli isolates from watermelon and melon in Israel. Plant Disease, 89, 1339–1347.

    Article  CAS  Google Scholar 

  • Dingle, K. E., Colles, F. M., Wareing, D. R. A., et al. (2001). Multilocus sequence typing system for campylobacter jejuni. Journal of Clinical Microbiology, 37(1), 14–23.

    Article  Google Scholar 

  • Enright, M. C., & Spratt, B. G. (1998). A multilocus sequence typing scheme for streptococcus pneumoniae: identification of clones associated with serious invasive disease. Microbiology, 144, 3049–3060.

    Article  PubMed  CAS  Google Scholar 

  • Feil, E. J., Li, B. C., Aanensen, D. M., et al. (2004). EBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. Journal of Bacteriology, 186(5), 1518–1530.

    Article  PubMed  CAS  Google Scholar 

  • Feng, J. J. (2008) The Molecular Detection and Genetic Diversity Analysis of Acidovorax avenae subsp.citrulli. [Ph. D. Dissertation]. Beijing: China Agricultural University

  • Feng, J. J., Schuenzel, E. L., Li, J. Q., et al. (2009). Multilocus sequence typing reveals two evolutionary lineages of acidovorax avenae subsp. Citrulli. Phytopathology, 99, 913–920.

    Article  PubMed  CAS  Google Scholar 

  • Homan, W. L., Tribe, D., Poznanski, S., et al. (2002). Multilocus sequence typing scheme for Enterococcus faecium. Journal of Clinical Microbiology, 40(6), 1963–1971.

    Article  PubMed  CAS  Google Scholar 

  • Maiden, M. C. J., Bygraves, J. A., Feil, E., et al. (1998). Multilocus sequence typing: a protable approach to the identification of clones within populations of pathogenic microorganisms. Proceedings of the National Academy of Sciences of the United States of America, 95, 3140–3145.

    Article  PubMed  CAS  Google Scholar 

  • McCombie, R. L., Finkelstein, R. A., & Woods, D. E. (2006). Multilocus sequence typing of historical Burkholderia pseudomallei isolates collected in southeast Asia from 1964 to 1967 provides insight into the epidemiology of melioidosis. Journal of Clinical Microbiology, 44(8), 2951–2962.

    Article  PubMed  CAS  Google Scholar 

  • O’Brien, R. G., & Martin, H. L. (1999). Bacterial blotch of melons caused by strains of acidovorax avenae subsp. citrulli. Australian Journal of Experimental Agriculture, 39, 479–485.

    Article  Google Scholar 

  • Ribot, E. M., Fair, M. A., Gautom, R., et al. (2006). Standardization of pulsed-field gel electrophoresis protocols for the bubtyping of Escherichia coli O157: H7, salmonella, and shigella for PulseNet. Foodborne Pathogens and Disease, 3(1), 59–67.

    Article  PubMed  CAS  Google Scholar 

  • Scally, M., Schuenzel, E. L., Ctouthamer, R., et al. (2005). Multilocus sequence type system for the plant pathogen xylella fastidiosa and relative contributions of recombination and point mutation to clonal diverisity. Applied and Environmental Microbiology, 71(12), 8491–8499.

    Article  PubMed  CAS  Google Scholar 

  • Schaad, N. W., Sowell, G., Goth, R. W., et al. (1978). Pseudomonas pseudolcaligenes subsp. Critrulli subsp. nov. International Journal of Systematic Bacteriology, 28(1), 117–125.

    Article  Google Scholar 

  • Schaad, N. W., Postnikova, E., Sechler, A., et al. (2008). Reclassification of subspecies of acidovorax avenae as A. Avenae (manns 1905) emend., A.Cattleyae (pavarino, 1911) comb. nov., A. citrulli (schaad et al., 1978) comb. nov., and proposal of A. Oryzae sp. nov. Systematic and Applied Microbiology, 31, 434–446.

    Article  PubMed  CAS  Google Scholar 

  • Somodi, G. C., Jones, J. B., Hopkins, D. L., et al. (1991). Occurrence of a bacterial watermelon fruit blotch in Florida. Plant Disease, 75(10), 1053–1056.

    Article  Google Scholar 

  • Urwin, R., & Maiden, M. C. J. (2003). Multi-locus sequence typing: a tool for global epidemiology. Trends in Microbiology, 11(10), 479–487.

    Article  PubMed  CAS  Google Scholar 

  • Walcott, R. R., Langston, D. B., Sanders, F. H., et al. (2000). Investigating intraspecific variation of Acidovoraxavenae subsp.citrulli using DNA fingerprinting and whole cell fatty acid analysis. Phytopathology, 90, 191–196.

    Article  PubMed  CAS  Google Scholar 

  • Walcott, R. R., Fessehaie, A., & Castro, A. C. (2004). Differences in pathogenicity between two genetically distinct groups of Acidovorax avenae subsp. citrulli on cucurbit hosts. Phytopathology, 152, 277–285.

    Article  Google Scholar 

  • Wen, A., Mangravita-Novo, A., Hopkins, D. L., et al. (2008). Genetic characterization of Acidovorax avenae subsp. citrulli using amplified fragment length polymorphism(AFLP). Phytopathology, 98(suppl), S169.

    Google Scholar 

  • Willems, A., Goor, M., Thielemans, S., et al. (1992). Transfer of several phytopathogenic Pseudomonas species to Acidovorax as Acidovorax avenae subsp. avenae subsp. nov., comb. nov., Acidovorax avenae subsp. citruli, Acidovorax avenae subsp. cattleyae, and Acidovorax konjaci. International Journal of Systematic Bacteriology, 42, 107–119.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, S. M., & Xu, J. G. (2008). Multilocus sequence typing and its application. Disease Surveillance, 23(10), 648–650. in Chinese.

    CAS  Google Scholar 

  • Zhao, T., Sun, F., Wang, B., et al. (2001). Pathogen identification of Hami melon bacterial fruit blotch. Acta Phytopathologica Sinica, 31(4), 357–364.

    Google Scholar 

  • Zhao, T., Feng, J., Sechler, A., et al. (2009). An improved assay for detection of Acidovorax citrulli in watermelon and melon seed. Seed Science and Technology, 37, 337–349.

    Google Scholar 

Download references

Acknowledgments

The earmarked fund for Modern Agro-industry Technology Research System (CARS-26) and Special Fund for Agro-scientific Research in the Public Interest (201003066).

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Authors and Affiliations

  1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Shasha Yan, Yuwen Yang, Tielin Wang & Tingchang Zhao

  2. USDA ARS, Foreign Disease-Weed Science Reserch Unit, 1301 Ditto Avenue, Ft. Detrick, MD, 21702-5023, USA

    Norman W. Schaad

Authors
  1. Shasha Yan
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  2. Yuwen Yang
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  3. Tielin Wang
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  4. Tingchang Zhao
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  5. Norman W. Schaad
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Correspondence to Tingchang Zhao.

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Yan, S., Yang, Y., Wang, T. et al. Genetic diversity analysis of Acidovorax citrulli in China. Eur J Plant Pathol 136, 171–181 (2013). https://doi.org/10.1007/s10658-012-0152-9

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