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Teratosphaeria (Mycosphaerella) nubilosa, the causal agent of Mycosphaerella leaf disease (MLD), recently introduced into Uruguay

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Abstract

Mycosphaerella leaf disease on Eucalyptus is well known in Uruguay but none of the more serious Mycosphaerella spp. and Teratosphaeria spp. causing this disease have yet been found. In the autumn of 2007, more severe defoliation than has been known in the past and associated with symptoms resembling Mycosphaerella infections was observed on Eucalyptus globulus. Isolations and identifications based on morphology and DNA sequence comparisons showed that the causal agent of the defoliation is the well known and serious pathogen Teratosphaeria nubilosa (=Mycosphaerella nubilosa). This is the first record of the pathogen in South America. Using ten microsatellite loci previously developed for T. nubilosa, only one multilocus haplotype was found from 46 T. nubilosa collected isolates. Interestingly, this haplotype was the same as one previously found in Portugal and Spain. The results suggest that T. nubilosa has recently been introduced into Uruguay and that it most likely originated from the Iberian Peninsula where E. globulus is widely planted.

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Abbreviations

MLD:

Mycosphaerella leaf disease

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Acknowledgements

We thank the National Research Foundation (NRF), members of the Tree Protection Co-operative Programme (TPCP) and the Department of Science and Technology/ NRF Centre of Excellence in Tree Health Biotechnology (CTHB), South Africa, for financial support.

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Authors and Affiliations

  1. Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa

    Guillermo Pérez, Gavin C. Hunter & Michael J. Wingfield

  2. CBS, Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT, Utrecht, Netherlands

    Gavin C. Hunter

  3. Department of Genetics, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, 0002, South Africa

    Bernard Slippers & Brenda D. Wingfield

  4. Departamento de Protección Vegetal, Facultad de Agronomía, Universidad de la República, Ruta 3 Km 363, Paysandú, CP60000, Uruguay

    Carlos Pérez

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  1. Guillermo Pérez
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  2. Gavin C. Hunter
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  3. Bernard Slippers
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  4. Carlos Pérez
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Correspondence to Guillermo Pérez.

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Pérez, G., Hunter, G.C., Slippers, B. et al. Teratosphaeria (Mycosphaerella) nubilosa, the causal agent of Mycosphaerella leaf disease (MLD), recently introduced into Uruguay. Eur J Plant Pathol 125, 109–118 (2009). https://doi.org/10.1007/s10658-009-9463-x

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