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AFLP analysis of genetic diversity in populations of Botrytis elliptica and Botrytis tulipae from the Netherlands

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Abstract

The objective of this study was to assess the genetic diversity and to infer the mode of reproduction of Botrytis elliptica and B. tulipae in the Netherlands. First, three molecular typing methods were compared for their ability to differentiate isolates of B. tulipae, B. elliptica, and B. cinerea. The methods compared were multilocus sequencing, restriction analysis of the ribosomal intergenic spacer (IGS) region, and amplified fragment length polymorphism (AFLP) analysis. AFLP fingerprinting provided the most efficient method to differentiate isolates within each Botrytis species and therefore this method was used for population analyses of B. elliptica and B. tulipae. Isolates of both species were sampled during successive growing seasons in experimental field plots in Lisse and other locations in the Netherlands. Among 174 B. elliptica isolates, 105 genotypes could be discriminated and 87 genotypes were found only once, reflecting high genotypic variation. Clonal genotypes were found only within growing seasons and in one location. Linkage disequilibrium analyses indicated that between 9.4% and 19.3% of the loci in clone-corrected samples were linked. The multilocus association index provided no evidence for random mating. We conclude that sexual recombination occurs in the B. elliptica population. Among the 170 B. tulipae isolates, 25 genotypes could be discriminated and four genotypes were found only once, reflecting a low genotypic variation. Clonal genotypes were frequently found in different growing seasons and different locations. Linkage disequilibrium analyses indicated that between 25.2% and 48.6% of the loci in clone-corrected samples were linked. We conclude that the B. tulipae population is mainly clonal with some recombination.

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Acknowledgements

The authors would like to thank Dr. Kim M. Plummer and Dr. Ioannis Stergiopoulos for critically reading the manuscript. We are grateful to Marjan de Boer and Ineke Pennock (PPO Lisse) for allowing us to sample in their experimental field and for excellent technical advice. Fien Meijer-Dekens, Petra van den Berg, Marleen Höfte, David Tena Marin and Ronald Wilterdink are acknowledged for technical assistance. This research was funded by the Dutch Technology Foundation STW, applied science division of NWO and the technology programme of the Ministry of Economic Affairs (project WEB5564).

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  1. M. Staats

    Present address: ENZA Seeds, Enkhuizen, The Netherlands

  2. P. van Baarlen

    Present address: Centre for Molecular and Biomolecular Informatics, Radboud University, Nijmegen, The Netherlands

Authors and Affiliations

  1. Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands

    M. Staats, P. van Baarlen & J. A. L. van Kan

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Staats, M., van Baarlen, P. & van Kan, J.A.L. AFLP analysis of genetic diversity in populations of Botrytis elliptica and Botrytis tulipae from the Netherlands. Eur J Plant Pathol 117, 219–235 (2007). https://doi.org/10.1007/s10658-006-9085-5

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