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Biodegradation of Di-n-butyl phthalate in rhizosphere and growth-promoting effect of Cucumis sativus Linn. by a novel Pseudomonas sp. DNB-S1

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Abstract

Plant associated-bacteria can facilitate the host plant in overcoming contaminant-induced stress responses as well as improve plant development and growth. In this study, a successful approach was reported to reduce the Dibutyl phthalate (DBP) levels of polluted soil and, consequently, to improve cucumber growth. DBP suppressed development of cucumber seedings significantly, damage sub-cellular of root, especially the biomembrane system, and affected the microbial community structures of the soil. When DBP was applied at a concentration of 5 mg/kg to cucumber seedlings inoculated with degrading strain DNB-S1, the DBP residue in roots was very low. When the cucumber plants were exposed to DBP stress over 20 and 40 mg/kg DBP, the DBP residues in the roots inoculated with degrading strain DNB-S1 were reduced by 36.5% and 40.42% respectively, compared with the non-inoculation group. Moreover, DBP dissipation in rhizosphere soil is accelerated through inoculation with DNB-S1 which could effectively relieve the pressure of DBP stress on plant. The dry weight of cucumber roots inoculated with DBP-degrading bacterium was higher than that of non-inoculated seedlings. According to ultrastructural micrographs, the DBP-degrading bacteria could considerably alleviate the damaging effect of DBP on cucumber root cell organs. The application of strain DNB-S1 could efficiently alleviated the stress of DBP on the microbial community structure.

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Acknowledgements

This work was supported by the National Natural Science Fund for Distinguished Young Scholars (41625002), the MOA Modern Agricultural Talents Support Project, the Second Batch of National “10,000 Person Plan” in 2016, the National Natural Science Foundation of China (41877128), the National Natural Science Foundation of China (31972941), the National Natural Science Foundation of China (31901189), the National Natural Science Foundation of China (41961144030), the major projects of research and development program of application technology of Heilongjiang Province of China (GA19B105), Heilongjiang Provincial Key Laboratory of Soil Protection and Remediation.

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Authors and Affiliations

  1. School of Resources & Environment, Northeast Agricultural University, Harbin, 150030, China

    Shuqin Li, Lei Wang, Ying Li, Fuxin Huang, Hui Yu, Ying Zhang, Rui Li, Ningrui Wei & Zhengzhe Yu

  2. College of Environment and Resources, Dalian Minzu University, 18 Liaohe West Road, Dalian, 116600, China

    Zhaobo Chen

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  1. Shuqin Li
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  2. Lei Wang
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  3. Ying Li
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Correspondence to Ying Zhang.

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Li, S., Wang, L., Li, Y. et al. Biodegradation of Di-n-butyl phthalate in rhizosphere and growth-promoting effect of Cucumis sativus Linn. by a novel Pseudomonas sp. DNB-S1. Ecotoxicology 30, 1454–1464 (2021). https://doi.org/10.1007/s10646-020-02287-0

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