Skip to main content
Log in

Identification of differentially expressed genes from contaminant and thermal exposed goldfish Carassius auratus in Gaobeidian Lake in Beijing, China

  • Published:
Ecotoxicology Aims and scope Submit manuscript

Abstract

Gaobeidian Lake, located in Beijing, China, derives its water mainly from the effluent of the Gaobeidian Wastewater Treatment Plant, which is moderately polluted. Additionally, as this water is used as a coolant in the nearby thermal power plant, the water of this lake has an elevated temperature. To screen differential gene expression in Gaobeidian Lake, suppressive subtractive hybridization (SSH) methodology was performed on RNA in goldfish Carassius auratus hepatic tissues from the lake, using Huairou Reservoir as reference site. A total of 768 candidate clones were selected to perform differential screening. Of these, 264 clones were differentially expressed between the two sites, 124 of which were then subjected to DNA sequencing. Consequently, 36 different genes with known functions were obtained, and some of these differential genes were further confirmed by semiquantitative RT-PCR experiments. Many genes related to detoxification, stress and immune response, and metabolism, such as glutathione S-transferase (GST), cytochrome P450, family 2, subfamily b, polypeptide 10 (CYP2B10), CYP2X10, α-1-antitrypsin, and apolipoprotein A-I (Apo-AI), had higher expression levels in goldfish hepatic tissue from Gaobeidian Lake than those from the reference site. A set of nine genes with known functions were downregulated in Gaobeidian Lake compared to the reference site. The results provided evidence that organisms inhabiting Gaobeidian Lake were suffering a complex stress process and showing metabolism changes and disturbance of homeostasis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Bartosiewicz M, Penn S, Buckpitt A (2001) Applications of gene arrays in environmental toxicology: fingerprints of gene regulation associated with cadmium chloride, benzo(a)pyrene, and trichloroethylene. Environ Health Perspect 109:71–74

    Article  CAS  Google Scholar 

  • Bly JE, Clem LW (1992) Temperature and teleost immune functions. Fish Shellfish Immunol 2:159–171

    Article  Google Scholar 

  • Delanghe JR, Langlois MR (2001) Hemopexin: a review of biological aspects and the role in laboratory medicine. Clin Chim Acta 312:13–23

    Article  CAS  Google Scholar 

  • Diatchenko L, Lau YF, Campbell AP, Chenchik A, Moqadam F, Huang B, Lukyanov S, Lukyanov K, Gurskaya N, Sverdlov ED, Siebert PD (1996) Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc Natl Acad Sci USA 93:6025–6030

    Article  CAS  Google Scholar 

  • Eaton DL, Bammler TK (1999) Concise review of the glutathione S-transferases and their significance to toxicology. Toxicol Sci 49:156–164

    Article  CAS  Google Scholar 

  • Hanlon PR, Zheng W, Ko AY, Jefcoate CR (2005) Identification of novel TCDD-regulated genes by microarray analysis. Toxicol Appl Pharmacol 202:215–228

    Article  CAS  Google Scholar 

  • Hayes JD, Pulford DJ (1995) The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol 30:445–600

    CAS  Google Scholar 

  • Hirayama M, Kobiyama A, Kinoshita S, Watabe S (2004) The occurrence of two types of hemopexin-like protein in medaka and differences in their affinity to heme. J Exp Biol 207:1387–1398

    Article  CAS  Google Scholar 

  • Hirayama M, Nakaniwa M, Ikeda D, Hirazawa N, Otaka T, Mitsuboshi T, Shirasu K, Watabe S (2003) Primary structures and gene organizations of two types of Wap65 from the pufferfish Takifugu rubripes. Fish Physiol Biochem 29:211–224

    Article  CAS  Google Scholar 

  • Ji W, Wright MB, Cai L, Flament A, Lindpaintner K (2002) Efficacy of SSH PCR in isolating differentially expressed genes. BMC Genomics, 3:12

    Article  Google Scholar 

  • Kikuchi K, Watabe S, Aida K (1997) The Wap65 gene expression of goldfish (Carassius auratus) in association with warm water temperature as well as bacterial lipopolysaccharide (LPS). Fish Physiol Biochem 17:423–432

    Article  CAS  Google Scholar 

  • Kikuchi K, Watabe S, Suzuki Y, Aida K, Nakajima H (1993) The 65-kDa cytosolic protein associated with warm temperature acclimation in goldfish, Carassius auratus. J Comp Physiol B 163:349–354

    Article  CAS  Google Scholar 

  • Kikuchi K, Yamashita M, Watabe S, Aida K (1995) The warm temperature acclimation-related 65-kDa protein, Wap65, in goldfish and its gene expression. J Biol Chem 270:17087–17092

    Article  CAS  Google Scholar 

  • King YT, Lin CS, Lin JH, Lee WC (2002) Whole-body hyperthermia-induced thermotolerance is associated with the induction of heat shock protein 70 in mice. J Exp Biol 205:273–278

    CAS  Google Scholar 

  • Leaver MJ, Wright J, George SG (1997) Structure and expression of a cluster of glutathione S-transferase genes from a marine fish, the plaice (Pleuronectes plastessa). Biochem J 321:405–412

    CAS  Google Scholar 

  • Molmenti EP, Ziambaras T, Perlmutter DH (1993) Evidence for an acute phase response in human intestinal epithelial cells. J Biol Chem 268:14116–14124

    CAS  Google Scholar 

  • Nebert DW, Russell DW (2002) Clinical importance of the cytochromes P450. Lancet 360:1155–1162

    Article  CAS  Google Scholar 

  • Nelson DR, Koymans L, Kamataki T, Stegeman JJ, Feyereisen R, Waxman DJ, Waterman MR, Gotoh O, Coon MJ, Estabrook RW, Gunsalus IC, Nebert DW (1996) P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics 6:1–42

    Article  CAS  Google Scholar 

  • Nelson DR, Zeldin DC, Hoffman SM, Maltais LJ, Wain HM, Nebert DW (2004) Comparison of cytochrome P450 (CYP) genes from the mouse and human genomes, including nomenclature recommendations for genes, pseudogenes and alternative-splice variants. Pharmacogenetics 14:1–18

    Article  CAS  Google Scholar 

  • Pfeffer A, Rogers KM, O’Keeffe L, Osborn PJ (1993) Acute phase protein response, food intake, liveweight change and lesions following intrathoracic injection of yeast in sheep. Res Vet Sci 55:360–366

    CAS  Google Scholar 

  • Pham RT, Barber DS, Gallagher EP (2004) GSTA is a major glutathione S-transferase gene responsible for 4-hydroxynonenal conjugation in largemouth bass liver. Mar Environ Res 58:485–488

    Article  CAS  Google Scholar 

  • Prophete C, Carlson EA, Li Y, Duffy J, Steinetz B, Lasano S, Zelikoff JT (2006) Effects of elevated temperature and nickel pollution on the immune status of Japanese medaka. Fish Shellfish Immunol 21:325–334

    Article  CAS  Google Scholar 

  • Rau MA, Whitaker J, Freedman JH, Di Giulio RT (2004) Differential susceptibility of fish and rat liver cells to oxidative stress and cytotoxicity upon exposure to prooxidants. Comp Biochem Physiol C Toxicol Pharmacol 137:335–342

    Article  Google Scholar 

  • Steel DM, Whitehead AS (1994) The major acute phase reactants: C-reactive protein, serum amyloid P component and serum amyloid A protein. Immunol Today 15:81–88

    Article  CAS  Google Scholar 

  • Trute M, Gallis B, Doneanu C, Shaffer S, Goodlett D, Gallagher E (2006) Characterization of hepatic glutathione S-transferases in coho salmon (Oncorhynchus kisutch). Aquat Toxicol (Epub ahead of print)

  • Weibezahn J, Tessarz P, Schlieker C, Zahn R, Maglica Z, Lee S, Zentgraf H, Weber-Ban EU, Dougan DA, Tsai FT, Mogk A, Bukau B (2004) Thermotolerance requires refolding of aggregated proteins by substrate translocation through the central pore of ClpB. Cell 119:653–665

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was funded by 973 Program (2006CB403306) and the Innovation Project of the Chinese Academy of Sciences (No: SCX2-SW-128 to Dai).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jiayin Dai.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, J., Wei, Y., Li, X. et al. Identification of differentially expressed genes from contaminant and thermal exposed goldfish Carassius auratus in Gaobeidian Lake in Beijing, China. Ecotoxicology 16, 525–532 (2007). https://doi.org/10.1007/s10646-007-0159-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10646-007-0159-8

Keywords

Navigation