Abstract
To understand whether direct transmission of H. pylori occurs from infected mouse to noninfected mouse, the system using a mouse model we developed previously was tested. Six nude mice were challenged with H. pylori inocula; one group consisted of one challenged nude mouse 1 week after inoculation raised with four nonchallenged nude mice in a single cage. For the single cage, a polycarbonate cage or a mesh-floor cage was used. Then three groups were kept in a polycarbonate cage and the other three groups kept in a mesh-floor cage to avoid H. pylori transmission through stool. After coraising for 1, 2, or 3 weeks, all mice were sacrificed to determine the existence of H. pylori in the stomach, saliva, and stool by culture or PCR and H. pylori-associated gastritis. RAPD fingerprinting patterns using different primers of isolated strains from challenged and nonchallenged mice were compared to understand the origin of transmitted strains. During 3 weeks after coraising of H. pylori challenged and nonchallenged mice, H. pylori was detected in the stomachs in 3 of 12 nonchallenged mice in the polycarbonate cage and in 2 of 12 nonchallenged mice in the cage with a steel mesh floor. H. pylori was detected from saliva or stool in two nonchallenged, infected mice in the polycarbonate cage. Moreover, RAPD fingerprinting using different primers of the total five strains isolated from five nonchallenged, infected mice in both cages showed the same pattern and concordance with that of the challenged strain and the strains isolated from challenged mice. It is demonstrated that intimate interaction is the cause of H. pylori transmission via saliva and stool.
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Jones DM, Eldridge J, Whorwell PJ: Antibodies to Campylobacter pyloridis in household contacts of infected patients. Br Med J 294:615, 1987
Ferguson DA JR, Li C, Patel NR, Mayberry WR, Chi DS, Thomas E: Isolation Helicobacter pylori from saliva. J Clin Microbiol 31:2802–2804, 1993
Karajden S, Fuksa M, Anderson J, Kempston J, Boccia A, Petrea C, Babida C, Karmali M, Penner JL: Examination of human stomach biopsies, saliva, and dental plaque for Campylobacter pylori. J Clin Microbiol 27:1397–1398, 1989
Fiedorek SC, Malaty HM, Evans DG, Pumphrey CL, Casteel HB, Evans DJ Jr, Graham DY: Factors influencing the epidemiology of Helicobacter pylori infection in children. Pediatrics 88:578–582, 1991
Rothenbacher D, Bode G, Berg G, Knayer U, Gonser T, Adler G, Brenner H: Helicobacter pylori among preschool children and their parents: evidence of parent-child transmission. J Infect Dis 179:398–402, 1999
Goodman KJ, Correa P: Transmission of Helicobacter pyloriamong siblings. Lancet 355:358–362, 2000
Han SR, Zschausch HCE, Meyer HGW, Schneider T, Loos M, Bhankdi S, Maeurer M: Helicobacter pylori: clonal population structure and restricted transmission within families revealed by molecular typing. J Clin Microbiol 38:3646–3651, 2000
Drumm B, Perez-Perez GI, Blaser MJ, Sherman PM: Intrafamilial clustering of Helicobacter pylori infection. N Engl J Med 322:359–363, 1990
Hulten K, Han SW, Enroth H, Klein PD, Opekun AR, Gilman AR, Gilman RH, Evans DG, Engstrand L, Graham DY, El-Zaatari FAK: Helicobacter pylori in the drinking water in Peru. Gastroenterolgy 110:1031–1035, 1996
Asaka M, Kimura T, Kudo M, Takeda H, Mitani S, Miyazaki T, Miki K, Graham DY: Relationship of Helicobacter pylori to serum pepsinogens in an asymptomatic Japanese population. Gastroenterology 102:760–766, 1992
Karita M., Teramukai S, Matsumoto S: The transmission route of Helicobacter pylori infection: The importance of the history of drinking well water-. Helicobacter Res 8:21–29, 2004 (in Japanese)
Karita M, Teramukai S, Matsumoto S: Risk of Helicobacter pylori transmission from drinking well water is higher than that from infected intrafamilial members in Japan. Dis Dis Sci 48:1062–1067, 2003
Karita M, Kouchiyama T, Okita K, Nkazawa T: New small animal model for human gastric Helicobacter pylori infection: success in both nude and euthymic mouse. Am J Gastroenterol 86:1596–1603, 1991
Karita M., Li Q, Okita K: Evaluation of new therapy for eradication of H. pylori infection in nude mouse model. Am J Gastroenterol 88:1366–1372, 1993
Karita M., Li Q, Cantero D, Okita K: Establishment of a small animal model for human gastric Helicobacter pylori infection using germ-free mouse. Am J Gastroenterol 89:208–213, 1994
Karita M: Bacteriology of Helicobacter pylori infection and analysis of the route of its trasmission. Shindan to Chiryou 82:1251–1256, 1994 (in Japanese)
Evans DG, Karjalainen TK, Evans DJ Jr, Graham DY, Lee CH: Cloning, nucleotide sequence, and expression of a gene encoding an adhesin subunit protein of Helicobacter pylori. J Bacteriol 175:674–683, 1993
Thomas JE, Gibson GR, Darboe MK, Dale A, Weaver LT: Isolation of Helicobacter pylori from human faeces. Lancet 340:1194–1195, 1992
Karita M, Tummuru MKR, Wirth HP, Blaser MJ: Effect of growth phase and acid shock on Helicobacter pylori cagA expression. Infect Immun 64:4501–4507, 1996
Akopyanz N, Bukanov NO, Westblom TU, Kresovich S, Berg DE: DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting. Nucleic Acids Res 20:5137–5142, 1992
Yoshimatsu T, Shirai M, Nagata K, Okita K, Nakazawa T: Transmission of Helicobacter pylori from challenged to nonchallenged nude mice kept in a single cage. Dis Dis Sci 45:1747–1753, 2000
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Karita, M., Matsumoto, S., Kamei, T. et al. Direct Transmission of H. pylori from Challenged to Nonchallenged Mice in a Single Cage. Dig Dis Sci 50, 1092–1096 (2005). https://doi.org/10.1007/s10620-005-2710-x
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DOI: https://doi.org/10.1007/s10620-005-2710-x