Abstract
Melanoma is a predominant cause of skin cancer-related deaths. It was reported that, the methanolic extract of Pouzolzia Indica (P. indica) on chromatography gave five compounds (1-hentriacontanyl palmitate, myricyl alcohol, 6,7-dimethoxycoumarin, trichadonic acid and friedelane), which inhibited the acute promyelocytic leukemia cell lines, NB4, and HT93A. Friedelane was extracted as active compound from methanolic extract of P. indica. In this study, friedelane was tested on murine metastatic B16F10 and B16BL6 melanoma cell lines. To achieve the target, the cell viability using trypan blue exclusion, acridine orange/EtBr staining and cell cytotoxicity were tested using MTT assay. Caspase-3, caspase-9, Cyt-c, BAD and Bax protein were assayed to evidence the apoptosis induction. The compound friedelane shows potent cytotoxic effect against metastatic melanoma mouse cell lines in 10 µg/ml concentration.
Similar content being viewed by others
References
Ahmed A, Rajendaran JD, Singh HP, Mishra A, Chandra D, Yadav IK, Jain DA (2010) Anti-snake venom activity of different extracts of Pouzolzia indica against russel viper venom. Int J ChemTech Res 2:744–751
Akihisa T, Yamamoto K, Tamura T, Kimura Y, Iida T, Nambara T, Chang FC (1992) Triterpenoid ketones from Lingnania chungii McClure: arborinone, friedelin and glutinone. Chem Pharm Bull 40:789–791
Ardilesa AE, González-Rodríguezb A, Núñeza MJ, Peresteloa NR et al (2012) Studies of naturally occurring friedelane triterpenoids as insulin sensitizers in the treatment type 2 diabetes mellitus. Phytochem 84:116–124
Dey SK, Bose D, Hazra A, Naskar S, Nandy A, Munda RN et al (2013) Cytotoxic activity and apoptosis-inducing potential of di-spiropyrrolidino and di-spiropyrrolizidino oxindole andrographolide derivatives. PLoS ONE 8:e58055
Farha AK, Geetha BS, Nair MS, Dhanya SR, Latha PG, Andremani P (2013) Apoptosis mediated cytotoxicity induced by isodeoxyelephantopin on nasopharyngeal carcinoma cells. Asian J Pharm Clin Res 6:51–56
Figueiredo CR, Matsuo AL, Massaoka MH, Girola N, Azevedo RA et al (2014) Antitumor activity of Kielmeyera coriacea leaf constituents in experimental melanoma, tested in vitro and in vivo in syngeneic mice. Adv Pharm Bull 4:429–436
Fridman JS, Lowe SW (2003) Control of apoptosis by p53. Oncogene 22:9030–9040. https://doi.org/10.1038/sj.onc.1207116
Ghani A (2003) Medicinal plants of Bangladesh with chemical constituents and uses, 2nd edn. Asiatic Society of Bangladesh, Dhaka
Ku B, Liang C, Jung JU, Oh BH (2011) Evidence that inhibition of BAX activation by BCL-2 involves its tight and preferential interaction with the BH3 domain of BAX. Cell Res 21:627–641
Kwok AKH, Yeung C-K, Lai TYY, Chan K-P, Pang CP (2004) Effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells. Br J Ophthalmol 88:1590–1594. https://doi.org/10.1136/bjo.2004.044537
Li L, Huang X, Sattler I, Fu H, Grabley S, Lin W (2006) Structure elucidation of a new friedelane triterpene from the mangrove plant Hibiscus tiliaceus. Magn Reson Chem 44:624–628
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with folin phenol reagent. J Biol Chem 193:265–275
Olmedo DA, López-Pérez JL, del Olmo E, Vásquez Y, San Feliciano A, Gupta MP (2008) A new cytotoxic friedelane acid–pluricostatic acid–and other compounds from the leaves of Marila pluricostata. Molecules 13:2915–2924. https://doi.org/10.3390/molecules13112915
Ribble D, Goldstein NB, Norris DA, Shellman YG (2005) A simple technique for quantifying apoptosis in 96-well plates. BMC Biotechnol 5:12
Roongruangchai K, Kummalue T, Sookkua T, Roongruangchai J (2010) Comparison of Pouzolzia indica methanolic extract and Virkon against cysts of Acanthamoeba spp. Southeast Asian J Trop Med Public Health 41:776–784
Sangsuwon C, Jiratchariyakul W, U-pratya Y, Kummalue T (2013) Antiproliferative effect and the isolated compounds of Pouzolzia indica. Evid Based Complement Altern Med 2013:342352. https://doi.org/10.1155/2013/342352
Sil Sarma I, Dinda B (2013) A new friedelane triterpene ester from Pouzolzia indica. Indian J Chem 52B:1527–1530
Solowey E, Lichtenstein M, Sallon S, Paavilainen H, Solowey E, Lorberboum-Galski H (2014) Evaluating medicinal plants for anticancer activity. Sci World J 2014:721402. https://doi.org/10.1155/2014/721402
Srisapoomi T, Jiratchariyakul W, Partkaittikul N, Kummalue T (2008) Effect of two Thai herbal remedies on the sensitivity of chemotherapeutic agents in human cancer cells. Asian J Tradit Med 3:108–111
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76:4350–4354
Trakulsomboon S, Kummalue T, Jiratchariyakul W (2006) Antibacterial activities of four Thai medicinal plants. J Med Assoc Thai 89:1466–1471
U-pratya Y, Jiratchariyakul W, Kummalue T (2008) Anti-proliferative effects of Pouzolzia indica on acute promyelocytic cell lines: NB4 and HT93A. Asian J Tradit Med 3:124–133
Acknowledgements
We are thankful to DBT for funding and State Biotech Hub, Tripura University for providing the instrumental facilities. We are also thankful to ICMR for providing fellowship to AKS to complete this work.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest for this study.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Maiti, D., Singha, A.K., Sarkar, C. et al. Friedelane, isolated from Pouzolzia indica Gaud. exhibits toxic effect against melanoma. Cytotechnology 70, 1111–1120 (2018). https://doi.org/10.1007/s10616-017-0174-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10616-017-0174-4