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Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development

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Abstract

This study aimed to assess the efficiency and effects of insulin-like growth factor receptor-1 (IGF-IR) siRNA knockdown during bovine preimplantation embryonic development. In oocytes injected with IGF-IR siRNA, the relative IGF-IR mRNA levels compared to controls were 28% and 46% at 6 and 24 h after injection, respectively. With respect to the injection of IGF-IR siRNA in zygotes, 24 h after injection the relative levels of IGF-IR mRNA and protein in the two-cell embryos were 74% and 78% of those in the controls, respectively. IGF-IR siRNA reduced blastocyst formation (23.2%) compared to siRNA controls (33.0%) and uninjected oocytes (35.4%; P < 0.05) and the number of viable cells per IGF-IR siRNA-treated blastocyst (64 ± 3) was significantly reduced, compared to control siRNA and uninjected blastocysts (81 ± 3 and 116 ± 4; P < 0.01). In conclusion, IGF-IR siRNA knockdown reduces the development of bovine embryos, and microinjection in zygotes can decrease blastocyst cell number.

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Acknowledgments

The present study was supported by grants from the Natural Science Foundation of Inner Mongolia of China (No. 200711020401), the National High Technology Research and Development Program (“863”Program) of China (No. 2008AA1010070) and the National Natural Science Foundation of China (NNSF No. 30760168).

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Authors and Affiliations

  1. Key Laboratory of China Education Ministry for Research of Mammal Reproductive Biology and Biotechnology, Inner Mongolia University, Hohhot, 010021, People’s Republic of China

    L. M. Wang, J. X. Wen, J. L. Yuan, Ming Cang & D. J. Liu

  2. Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, 832000, People’s Republic of China

    L. M. Wang

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  1. L. M. Wang
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  2. J. X. Wen
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  3. J. L. Yuan
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  4. Ming Cang
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  5. D. J. Liu
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Correspondence to D. J. Liu.

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L. M. Wang and J. X. Wen contributed equally to this work.

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Wang, L.M., Wen, J.X., Yuan, J.L. et al. Knockdown of IGF-IR by siRNA injection during bovine preimplantation embryonic development. Cytotechnology 64, 165–172 (2012). https://doi.org/10.1007/s10616-011-9402-5

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  • DOI: https://doi.org/10.1007/s10616-011-9402-5

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