Skip to main content
SpringerLink
Log in

Domain Organization of Lectin from the Mussel Crenomytilus grayanus

  • Published:
Chemistry of Natural Compounds Aims and scope

The three-dimensional structure is critical to the functioning of many proteins. A combination of several methods (hemagglutination, gel chromatography, solid-phase lectin-based enzyme-linked assay, and SDS electrophoresis) was used to establish the role of the collagen-like domain during self-association of lectin from the sea mussel Crenomytilus grayanus (CGL). Oligomerization of CGL through its collagen-like domain was important for the manifestation of carbohydrate-binding properties.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  1. N. I. Belogortseva, V. I. Molchanova, A. V. Kurika, A. S. Skobun, and V. E. Glazkova, Comp. Biochem. Physiol., Part C: Pharmacol., Toxicol., Endocrinol., 119, 45 (1998).

    CAS  Google Scholar 

  2. S. N. Kovalchuk, I. V. Chikalovets, O. V. Chernikov, V. I. Molchanova, W. Li, V. A. Rasskazov, and P. A. Lukyanov, Fish Shellfish Immunol., 35, 1320 (2013).

    Article  PubMed  CAS  Google Scholar 

  3. A. Broom, A. C. Doxey, Y. D. Lobsanov, L. G. Berthin, D. R. Rose, P. L. Howell, B. J. McConkey, and E. M. Meiering, Structure, 20, 161 (2012).

    Article  PubMed  CAS  Google Scholar 

  4. T. Hatakeyama, M. Furukawa, H. Nagatomo, N. Yamasaki, and T. Mori, J. Biol. Chem., 271, 16915 (1996).

    Article  PubMed  CAS  Google Scholar 

  5. M. Chou and R. D. Cummings, J. Biol. Chem., 270, 5198 (1995).

    Article  Google Scholar 

  6. B. Mehul, S. Bawumia, S. R. Martin, and R. C. Hughes, J. Biol. Chem., 269, 18250 (1994).

    PubMed  CAS  Google Scholar 

  7. B. Friguet, A. F. Chaffotte, L. Djavadi-Ohaniance, and M. E. Goldberg, J. Immunol. Methods, 77, 305 (1985).

    Article  PubMed  CAS  Google Scholar 

  8. S. M. Massa, D. N. W. Cooper, H. Leffler, and S. H. Barondes, Biochemistry, 32, 260 (1993).

    Article  PubMed  CAS  Google Scholar 

  9. M. E. Cromwell, E. Hilario, and F. Jacobson, AAPS J., 8, 572 (2006).

    Article  Google Scholar 

  10. U. Laemmli, Nature, 227, 680 (1970).

    Article  PubMed  CAS  Google Scholar 

  11. D. M. Bollag, M. D. Rozycki, and S. J. Edelstein, Protein Methods, 2nd Ed., Wiley-Liss Inc., New York, 1996, 415 pp.

    Google Scholar 

  12. P. K. Nakane and A. Lavaoi, J. Histochem. Cytochem., 22, 1084 (1974).

    Article  PubMed  CAS  Google Scholar 

  13. I. V. Chikalovets, O. V. Chernikov, E. A. Shekhova, V. I. Molchanova, and P. A. Luk’yanov, Biol. Morya, 36, 70 (2010).

    CAS  Google Scholar 

Download references

Acknowledgment

The work was supported financially by the RFBR (Grant No. 12-04-00862) and the Basic Research Program of the FEB RAS “Far East” P42.

Author information

Authors and Affiliations

  1. G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-East Branch, Russian Academy of Sciences, 690022, Vladivostok, Prosp. 100-Let Vladivostoku, 159, Russia

    I. V. Chikalovets, V. I. Molchanova, O. V. Chernikov & P. A. Luk’yanov

  2. Far-East Federal University, 690950, Vladivostok, Sukhanova, 8, Russia

    I. V. Chikalovets

  3. Pacific State Medical University, 690002, Vladivostok, Ostryakova, 2, Russia

    P. A. Luk’yanov

Authors
  1. I. V. Chikalovets
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. V. I. Molchanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. V. Chernikov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. A. Luk’yanov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to I. V. Chikalovets.

Additional information

Translated from Khimiya Prirodnykh Soedinenii, No. 4, July–August, 2014, pp. 613–616.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Chikalovets, I.V., Molchanova, V.I., Chernikov, O.V. et al. Domain Organization of Lectin from the Mussel Crenomytilus grayanus . Chem Nat Compd 50, 706–709 (2014). https://doi.org/10.1007/s10600-014-1057-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10600-014-1057-y

Keywords

Search

Navigation