Abstract
Yellow grouper (Epinephelus awoara) is a commercially important marine fish species. A dinucleotide-enriched genomic library of E. awoara was constructed using the method of FIASCO. Twelve loci were polymorphic in a test population with alleles per locus ranging from two to eight, and observed and expected heterozygosities per locus from 0.13 to 1.00 and from 0.20 to 0.86, respectively. Five loci significantly deviated from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found between pairs of loci. Cross-species amplification of these polymorphic microsatellite loci was performed in additional two related species. These polymorphic microsatellite loci would be useful for investigating genetic diversity of E. awoara and related species.
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Acknowledgement
This study was supported by State 863 High-technology R&D project of China (2006AA10A403), Taishan Scholar Project of Shandong Province, China and Scientific foundation of YSFRI, CAFS (2007-qn-13).
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L. Zhao and C. Shao have contributed equally to this work.
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Zhao, L., Shao, C., Liao, X. et al. Twelve novel polymorphic microsatellite loci for the Yellow grouper (Epinephelus awoara) and cross-species amplifications. Conserv Genet 10, 743–745 (2009). https://doi.org/10.1007/s10592-008-9635-9
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DOI: https://doi.org/10.1007/s10592-008-9635-9