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Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis

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Abstract

Eleven polymorphic microsatellite loci were characterized for the endangered conifer Taxus yunnanensis. Eight loci were isolated through SSR-anchored PCR, one locus was developed by cross-species amplification tests, while the last two loci were obtained from cross-species microsatellite sequences available in GenBank. Variability of these markers was tested in 48 individuals collected from its main distribution range in Southwest China. The number of alleles per locus ranged from 2 to 9, and the observed and expected heterozygosity varied from 0.000 to 0.625 and from 0.062 to 0.853, respectively. Ten of these eleven loci were significantly deviated from Hardy–Weinberg expectation, except TS07 which showed a distinct heterozygote excess. The availability of these new polymorphic microsatellite markers will provide an ideal marker system for detailed population genetics studies in T. yunnanensis and potentially also for closely related species.

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References

  • Chagné D, Chaumeil P, Ramboer A et al (2004) Cross-species transferability and mapping of genomic and cDNA SSRs in pines. Theor Appl Genet 109:1204–1214

    Article  PubMed  Google Scholar 

  • Collignon AM, van de Sype H, Favre JM (2002) Geographical variation in random amplified polymorphic DNA and quantitative traits in Norway spruce. Can J Forest Res 32:266–282

    Article  CAS  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    CAS  Google Scholar 

  • Fisher PJ, Gardner RC, Richardson TE (1996) Single locus microsatellites isolated using 5′ anchored PCR. Nucleic Acids Res 24:4369–4371

    Article  PubMed  CAS  Google Scholar 

  • Fu LG, Li N, Robert RM (1999) Taxaceae. Flora of China 4:89–96

    Google Scholar 

  • Huang CC, Chiang TY, Hsu TW (2007) Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA). Conserv Genet. doi:10.1007/s10592-007-9341-z

  • Kingston DG, Newman DJ (2007) Taxoids: cancer-fighting compounds from nature. Curr Opin Drug Discov Devel 10:130–144

    PubMed  CAS  Google Scholar 

  • Li SH, Zhang HJ, Yao P et al (2000) Rearranged taxanes from the bark of Taxus yunnanensis. J Nat Prod 63:1488–1491

    Article  PubMed  CAS  Google Scholar 

  • Pannacciulli FG, Piyapattanakorn S, Bishop JDD et al (2005) Isolation of highly polymorphic microsatellite markers from the intertidal barnacle Chthamalus montagui Southward. Mol Ecol Notes 5:641–643

    Article  CAS  Google Scholar 

  • Raymond M, Rousset F (1995) Genepop (Version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225

    Article  Google Scholar 

  • Shi QW, Oritani T, Sugiyama T et al (1999) Two new taxane diterpenoids from the seeds of the Chinese yew, Taxus yunnanensis J Asian Nat Prod Res 2:71–79

    Article  PubMed  CAS  Google Scholar 

  • Van der Nest MA, Steenkamp ET, Wingfield BD et al (2000) Development of simple sequence repeat (SSR) markers in Eucalyptus from amplified inter-simple sequence repeats (ISSR). Plant Breeding 119:433–436

    Article  Google Scholar 

  • Walter R, Epperson BK (2001) Geographic pattern of genetic variation in Pinus resinosa: area of greatest diversity is not the origin of postglacial populations. Mol Ecol 10:103–111

    Article  PubMed  CAS  Google Scholar 

  • Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeats (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176–183

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work is jointly funded by Ministry Science and Technology of China (2006BAD18B0303) and the Research Institute of Resource Insects in Chinese Academy of Forestry (RIRI200703Z). We thank KN Stölting (University of Zürich, Switzerland), Cheng-Ye Wang and Liang Xie (Kunming Institute of Zoology, CAS) for their help in preparing the manuscript.

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Authors and Affiliations

  1. Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Bailongshi, Kunming, 650224, China

    Ying-Chun Miao, Jian-Rong Su & Zhi-Jun Zhang

  2. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China

    Hui Li & Ya-Ping Zhang

  3. Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming, 650091, China

    Jing Luo & Ya-Ping Zhang

  4. School of Life Science, Yunnan University, Kunming, 650091, China

    Jing Luo

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  1. Ying-Chun Miao
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  2. Jian-Rong Su
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  3. Zhi-Jun Zhang
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  4. Hui Li
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  5. Jing Luo
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  6. Ya-Ping Zhang
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Correspondence to Jian-Rong Su.

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Miao, YC., Su, JR., Zhang, ZJ. et al. Isolation and characterization of microsatellite markers for the endangered Taxus yunnanensis . Conserv Genet 9, 1683–1685 (2008). https://doi.org/10.1007/s10592-008-9532-2

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  • DOI: https://doi.org/10.1007/s10592-008-9532-2

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